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Science 4 October 1991:
Vol. 254. no. 5028, pp. 94 - 97
DOI: 10.1126/science.1840705
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Articles

Science, Vol 254, Issue 5028, 94-97
Copyright © 1991 by American Association for the Advancement of Science

articles

A dominant negative form of transcription activator mTFE3 created by differential splicing

C Roman, L Cohn, and K Calame

Department of Biological Chemistry, University of California, Los Angeles 90024.

Transcription factor E3 (mTFE3) is a murine transcription activator that binds to the intronic enhancer of the immunoglobulin heavy chain gene. A naturally occurring splice product of mTFE3 messenger RNA (mRNA) lacked 105 nucleotides that encode an activation domain; both absolute and relative amounts of long and truncated mRNAs varied in different tissues. Cells were cotransfected with complementary DNAs that encoded the two mRNA forms in amounts that corresponded to the amounts of each mRNA found in different cells. Small changes in substoichiometric amounts of the truncated form of mRNA effected trans-dominant negative modulation of mTFE3 activity. These findings identify a function for differential splicing in the regulation of transcription factor activity.


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