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Science 4 October 1991:
Vol. 254. no. 5028, pp. 81 - 86
DOI: 10.1126/science.1925563
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Articles

Science, Vol 254, Issue 5028, 81-86
Copyright © 1991 by American Association for the Advancement of Science

articles

Detection of somatic DNA recombination in the transgenic mouse brain

M Matsuoka, F Nagawa, K Okazaki, L Kingsbury, K Yoshida, U Muller, DT Larue, JA Winer, and H Sakano

Division of Immunology, University of California, Berkeley 94720.

A DNA construct containing the bacterial beta-galactosidase gene (lacZ) was used to study somatic DNA recombination in the transgenic mouse brain. Recombination-positive areas of the adult brain were stained blue with X-gal, a substrate of beta-galactosidase. Blue-colored cells appeared soon after birth, and continued to emerge in postnatal tissue. Staining was prominent in sensory as opposed to motor regions of the brain, and was present in more than 70 discrete areas of the nervous system. The possibility of DNA rearrangement is discussed with respect to the development of the central nervous system.


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