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Science 4 October 1991:
Vol. 254. no. 5028, pp. 59 - 67
DOI: 10.1126/science.1925562
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Articles

Science, Vol 254, Issue 5028, 59-67
Copyright © 1991 by American Association for the Advancement of Science

articles

Large-scale and automated DNA sequence determination

T Hunkapiller, RJ Kaiser, BF Koop, and L Hood

NSF Science and Technology Center for Molecular Biotechnology, California Institute of Technology, Pasadena 91125.

DNA sequence analysis is a multistage process that includes the preparation of DNA, its fragmentation and base analysis, and the interpretation of the resulting sequence information. New technological advances have led to the automation of certain steps in this process and have raised the possibility of large-scale DNA sequencing efforts in the near future [for example, 1 million base pairs (Mb) per year]. New sequencing methodologies, fully automated instrumentation, and improvements in sequencing-related computational resources may render genome-size sequencing projects (100 Mb or larger) feasible during the next 5 to 10 years.


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