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Science 20 September 1991:
Vol. 253. no. 5026, pp. 1423 - 1425
DOI: 10.1126/science.1654595
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Articles

Science, Vol 253, Issue 5026, 1423-1425
Copyright © 1991 by American Association for the Advancement of Science

articles

Negative regulation of CD45 protein tyrosine phosphatase activity by ionomycin in T cells

HL Ostergaard and IS Trowbridge

Department of Cancer Biology, Salk Institute, San Diego, CA 92186.

CD45 is a leukocyte-specific, transmembrane protein tyrosine phosphatase (PTPase) required for T cell responsiveness. How the activity of PTPases is regulated in vivo is unclear. Treatment of murine thymocytes and a variety of murine T cell lines with the calcium ionophore ionomycin decreased CD45 PTPase activity. Ionomycin treatment also led to a decreased phosphorylation of serine residues in CD45. These results indicate that increased intracellular calcium modulates CD45 PTPase activity, demonstrating regulation of CD45 PTPase activity in vivo, and also implicate serine dephosphorylation as a possible mechanism.


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