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Science 20 September 1991:
Vol. 253. no. 5026, pp. 1415 - 1417
DOI: 10.1126/science.1716786
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Articles

Science, Vol 253, Issue 5026, 1415-1417
Copyright © 1991 by American Association for the Advancement of Science

articles

A mechanosensitive channel in whole cells and in membrane patches of the fungus Uromyces

XL Zhou, MA Stumpf, HC Hoch, and C Kung

Laboratory of Molecular Biology, University of Wisconsin, Madison 53706.

Bean leaf stomata provide a topographical signal that induces germlings of the phytopathogen Uromyces appendiculatus to develop specialized infection structures. Protoplasts from germ tubes of this fungus, when examined with patch-clamp electrodes, displayed the activities of a 600-picosiemen mechanosensitive ion channel. This channel passes a variety of cations, including Ca2+, and is blocked by Gd3+ at 50 micromolar. This channel could transduce the membrane stress induced by the leaf topography into an influx of ions, including Ca2+, that may trigger differentiation.


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Response.
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