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Science 20 September 1991:
Vol. 253. no. 5026, pp. 1405 - 1408
DOI: 10.1126/science.1910206
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Articles
Science, Vol 253, Issue 5026, 1405-1408
Copyright © 1991 by American Association for the Advancement of Science
Atomic force microscopy and dissection of gap junctions
JH Hoh,
R Lal,
SA John,
JP Revel,
and
MF Arnsdorf
Division of Biology, California Institute of Technology, Pasedena 91125.
An atomic force microscope (AFM) was used to study the structure of isolated hepatic gap junctions in phosphate-buffered saline (PBS). The thickness of these gap junctions appears to be 14.4 nanometers, close to the dimensions reported by electron microscopy (EM). When an increasing force is applied to the microscope tip, the top membrane of the gap junction can be "dissected" away, leaving the extracellular domains of the bottom membrane exposed. When such "force dissection" is performed on samples both trypsinized and fixed with glutaraldehyde, the hexagonal array of gap junction hemichannels is revealed, with a center-to-center spacing of 9.1 nanometers.
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