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Science 6 September 1991:
Vol. 253. no. 5024, pp. 1140 - 1143
DOI: 10.1126/science.1887237
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Articles

Science, Vol 253, Issue 5024, 1140-1143
Copyright © 1991 by American Association for the Advancement of Science

articles

Specific DNA binding by c-Myb: evidence for a double helix-turn-helix-related motif

OS Gabrielsen, A Sentenac, and P Fromageot

Laboratoire d'Ingenierie des Proteines, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.

The c-Myb protein is a sequence-specific DNA binding protein that activates transcription in hematopoietic cells. Three imperfect repeats (R1, R2, and R3) that contain regularly spaced tryptophan residues form the DNA binding domain of c-Myb. A fragment of c-Myb that contained the R2 and R3 regions bound specifically to a DNA sequence recognized by c-Myb plus ten additional base pairs at the 3' end of the element. The R2R3 fragment was predicted to contain two consecutive helix-turn-helix (HTH) motifs with unconventional turns. Mutagenesis of amino acids in R2R3 at positions that correspond to DNA-contacting amino acids in other HTH-containing proteins abolished specific DNA binding without affecting nonspecific DNA interactions.


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