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Science 16 August 1991:
Vol. 253. no. 5021, pp. 762 - 768
DOI: 10.1126/science.1876833

Articles

Science, Vol 253, Issue 5021, 762-768
Copyright © 1991 by American Association for the Advancement of Science


articles

Convergence of Ets- and notch-related structural motifs in a heteromeric DNA binding complex

CC Thompson, TA Brown, and SL McKnight

Howard Hughes Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210.

Analysis of the heteromeric DNA binding protein GABP has revealed the interaction of two distinct peptide sequence motifs normally associated with proteins located in different cellular compartments. The alpha subunit of GABP contains an 85-amino acid segment related to the Ets family of DNA binding proteins. The ETS domain of GABP alpha facilitates weak binding to DNA and, together with an adjacent segment of 37 amino acids, mediates stable interaction with GABP beta. The beta subunit of GABP contains four imperfect repeats of a sequence present in several transmembrane proteins including the product of the Notch gene of Drosophila melanogaster. These amino-terminal repeats of GABP beta mediate stable interaction with GABP alpha and, when complexed with GABP alpha, directly contact DNA. These observations provide evidence for a distinct biochemical role for the 33-amino acid repeats, and suggest that they may serve as a module for the generation of specific dimerization interfaces.


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Identification and Characterization of a Novel Ets-2-related Nuclear Complex Implicated in the Activation of the Human Interleukin-12 p40 Gene Promoter.
X. Ma, M. Neurath, G. Gri, and G. Trinchieri (1997)
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A Novel Cytosolic Calcium-independent Phospholipase A2 Contains Eight Ankyrin Motifs.
J. Tang, R. W. Kriz, N. Wolfman, M. Shaffer, J. Seehra, and S. S. Jones (1997)
J. Biol. Chem. 272, 8567-8575
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B. C. Villafuerte, W. Zhao, A. C. Herington, R. Saffery, and L. S. Phillips (1997)
J. Biol. Chem. 272, 5024-5030
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Redox Regulation of GA-binding Protein-alpha DNA Binding Activity.
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J. Biol. Chem. 271, 25617-25623
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D Fitzsimmons, W Hodsdon, W Wheat, S M Maira, B Wasylyk, and J Hagman (1996)
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GABP Mediates Insulin-increased Prolactin Gene Transcription.
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J. Biol. Chem. 271, 10425-10428
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I. E. Dulubova, V. G. Krasnoperov, M. V. Khvotchev, K. A. Pluzhnikov, T. M. Volkova, E. V. Grishin, H. Vais, D. R. Bell, and P. N. R. Usherwood (1996)
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GABP and PU.1 Compete for Binding, yet Cooperate to Increase CD18 (beta(2) Leukocyte Integrin) Transcription.
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Identification and Characterization of a Novel Cytokine-inducible Nuclear Protein from Human Endothelial Cells.
W. Chu, D. K. Burns, R. A. Swerlick, and D. H. Presky (1995)
J. Biol. Chem. 270, 10236-10245
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D S McNabb, Y Xing, and L Guarente (1995)
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Molecular and genetic characterization of GABP beta..
F C de la Brousse, E H Birkenmeier, D S King, L B Rowe, and S L McKnight (1994)
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Notch1 is essential for postimplantation development in mice..
P J Swiatek, C E Lindsell, F F del Amo, G Weinmaster, and T Gridley (1994)
Genes & Dev. 8, 707-719
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Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element..
M K Shin and M E Koshland (1993)
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Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ETS-domain proteins.
B Nelsen, G Tian, B Erman, J Gregoire, R Maki, B Graves, and R Sen (1993)
Science 261, 82-86
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Identity of GABP with NRF-2, a multisubunit activator of cytochrome oxidase expression, reveals a cellular role for an ETS domain activator of viral promoters..
J V Virbasius, C A Virbasius, and R C Scarpulla (1993)
Genes & Dev. 7, 380-392
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Altered epidermal growth factor-like sequences provide evidence for a role of Notch as a receptor in cell fate decisions.
P Heitzler and P Simpson (1993)
Development 117, 1113-1123
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Tissue-specific RNA splicing generates an ankyrin-like domain that affects the dimerization and DNA-binding properties of a bHLH protein..
E S Klein, D M Simmons, L W Swanson, and M G Rosenfeld (1993)
Genes & Dev. 7, 55-71
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Notch2: a second mammalian Notch gene.
G. Weinmaster, V. J. Roberts, and G. Lemke (1992)
Development 116, 931-941
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The proto-oncogene bcl-3 encodes an I kappa B protein..
L D Kerr, C S Duckett, P Wamsley, Q Zhang, P Chiao, G Nabel, T W McKeithan, P A Baeuerle, and I M Verma (1992)
Genes & Dev. 6, 2352-2363
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Specificities of protein-protein and protein-DNA interaction of GABP alpha and two newly defined ets-related proteins..
T A Brown and S L McKnight (1992)
Genes & Dev. 6, 2502-2512
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I kappa B interacts with the nuclear localization sequences of the subunits of NF-kappa B: a mechanism for cytoplasmic retention..
A A Beg, S M Ruben, R I Scheinman, S Haskill, C A Rosen, and A S Baldwin (1992)
Genes & Dev. 6, 1899-1913
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Interaction of murine ets-1 with GGA-binding sites establishes the ETS domain as a new DNA-binding motif..
J A Nye, J M Petersen, C V Gunther, M D Jonsen, and B J Graves (1992)
Genes & Dev. 6, 975-990
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I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity..
S M Ruben, J F Klement, T A Coleman, M Maher, C H Chen, and C A Rosen (1992)
Genes & Dev. 6, 745-760
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Cloning and expression in yeast of a plant potassium ion transport system.
H Sentenac, N Bonneaud, M Minet, F Lacroute, J. Salmon, F Gaymard, and C Grignon (1992)
Science 256, 663-665
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Expression of an activated Notch-related int-3 transgene interferes with cell differentiation and induces neoplastic transformation in mammary and salivary glands..
C Jhappan, D Gallahan, C Stahle, E Chu, G H Smith, G Merlino, and R Callahan (1992)
Genes & Dev. 6, 345-355
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Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells..
J H Xin, A Cowie, P Lachance, and J A Hassell (1992)
Genes & Dev. 6, 481-496
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T Xu, L. Caron, R. Fehon, and S Artavanis-Tsakonas (1992)
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   Abstract »    Full Text »    PDF »
Identification of Amino Acid Residues in the ETS Transcription Factor Erg That Mediate Erg-Jun/Fos-DNA Ternary Complex Formation.
A. Verger, E. Buisine, S. Carrere, R. Wintjens, A. Flourens, J. Coll, D. Stehelin, and M. Duterque-Coquillaud (2001)
J. Biol. Chem. 276, 17181-17189
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PAS kinase: An evolutionarily conserved PAS domain-regulated serine/threonine kinase.
J. Rutter, C. H. Michnoff, S. M. Harper, K. H. Gardner, and S. L. McKnight (2001)
PNAS 98, 8991-8996
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