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Science 2 August 1991:
Vol. 253. no. 5019, pp. 565 - 568
DOI: 10.1126/science.1857988
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Articles

Science, Vol 253, Issue 5019, 565-568
Copyright © 1991 by American Association for the Advancement of Science

articles

Differentiation of 3T3-L1 fibroblasts to adipocytes induced by transfection of ras oncogenes

M Benito, A Porras, AR Nebreda, and E Santos

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20892.

Mammalian 3T3-L1 cells differentiate into adipocytes after continuous exposure to pharmacological doses of insulin or physiological doses of insulin-like growth factor I (IGF-1). Expression of transfected ras oncogenes led to differentiation of these cells into adipocytes in the absence of externally added insulin or IGF-I. Cells transfected with normal ras genes or the tyrosine kinase trk oncogene did not differentiate. Transfection with a dominant inhibitory ras mutant resulted in inhibition of differentiation. Exposure of untransfected 3T3-L1 cells to insulin stimulated formation of the active Ras.GTP complex. These observations indicate that Ras proteins participate in signal transduction pathways initiated by insulin and IGF-I in these cells.


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