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Science 2 August 1991:
Vol. 253. no. 5019, pp. 557 - 560
DOI: 10.1126/science.1857985
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Articles

Science, Vol 253, Issue 5019, 557-560
Copyright © 1991 by American Association for the Advancement of Science

articles

Identification of profilin as a novel pollen allergen; IgE autoreactivity in sensitized individuals

R Valenta, M Duchene, K Pettenburger, C Sillaber, P Valent, P Bettelheim, M Breitenbach, H Rumpold, D Kraft, and O Scheiner

Institute of General and Experimental Pathology, University of Vienna, Austria.

A complementary DNA encoding a pollen allergen from white birch (Betula verrucosa) that was isolated from a pollen complementary DNA library with serum immunoglobulin E from a birch pollen-allergic individual revealed significant sequence homology to profilins. The recombinant protein showed high affinity to poly-L-proline. Immunoglobulin E antibodies from allergic individuals bound to natural and recombinant birch profilin and also to human profilin. In addition, birch and human profilin induced histamine release from blood basophils of profilin-allergic individuals, but not of individuals sensitized to other plant allergens. The structural similarity of conserved proteins might therefore be responsible for maintaining immunoglobulin E antibody titers in type I allergy.


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