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Science 2 August 1991:
Vol. 253. no. 5019, pp. 551 - 555
DOI: 10.1126/science.1857984

Articles

Science, Vol 253, Issue 5019, 551-555
Copyright © 1991 by American Association for the Advancement of Science


articles

A component of calcium-activated potassium channels encoded by the Drosophila slo locus

NS Atkinson, GA Robertson, and B Ganetzky

Laboratory of Genetics, University of Wisconsin, Madison 53706.

Calcium-activated potassium channels mediate many biologically important functions in electrically excitable cells. Despite recent progress in the molecular analysis of voltage-activated K+ channels, Ca(2+)-activated K+ channels have not been similarly characterized. The Drosophila slowpoke (slo) locus, mutations of which specifically abolish a Ca(2+)-activated K+ current in muscles and neurons, provides an opportunity for molecular characterization of these channels. Genomic and complementary DNA clones from the slo locus were isolated and sequenced. The polypeptide predicted by slo is similar to voltage-activated K+ channel polypeptides in discrete domains known to be essential for function. Thus, these results indicate that slo encodes a structural component of Ca(2+)-activated K+ channels.


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Large conductance voltage- and calcium-dependent K+ channel, a distinct member of voltage-dependent ion channels with seven N-terminal transmembrane segments (S0-S6), an extracellular N terminus, and an intracellular (S9-S10) C terminus.
P. Meera, M. Wallner, M. Song, and L. Toro (1997)
PNAS 94, 14066-14071
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Characterization of Gating and Peptide Block of mSlo, a Cloned Calcium-Dependent Potassium Channel.
D. A. Sullivan, M. H. Holmqvist, and I. B. Levitan (1997)
J Neurophysiol 78, 2937-2950
   Abstract »    Full Text »    PDF »
A human intermediate conductance calcium-activated potassium channel.
T. M. Ishii, C. Silvia, B. Hirschberg, C. T. Bond, J. P. Adelman, and J. Maylie (1997)
PNAS 94, 11651-11656
   Abstract »    Full Text »    PDF »
Redox Modulation of hslo Ca2+-Activated K+ Channels.
T. J. DiChiara and P. H. Reinhart (1997)
J. Neurosci. 17, 4942-4955
   Abstract »    Full Text »    PDF »
Presynaptic Recordings from Drosophila: Correlatin of Macroscopic and Single-Channel K+ Currents.
M. Martinez-Padron and A. Ferrus (1997)
J. Neurosci. 17, 3412-3424
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Voltage-controlled gating in a large conductance Ca2+-sensitive K+channel (hslo).
E. Stefani, M. Ottolia, F. Noceti, R. Olcese, M. Wallner, R. Latorre, and L. Toro (1997)
PNAS 94, 5427-5431
   Abstract »    Full Text »    PDF »
A Cysteine-rich Domain Defined by a Novel Exon in a Slo Variant in Rat Adrenal Chromaffin Cells and PC12 Cells.
M. Saito, C. Nelson, L. Salkoff, and C. J. Lingle (1997)
J. Biol. Chem. 272, 11710-11717
   Abstract »    Full Text »    PDF »



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