Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 26 July 1991:
Vol. 253. no. 5018, pp. 407 - 414
DOI: 10.1126/science.1862342
Prev | Table of Contents | Next

Articles

Science, Vol 253, Issue 5018, 407-414
Copyright © 1991 by American Association for the Advancement of Science

articles

Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase

DR Knighton, JH Zheng, LF Ten Eyck, VA Ashford, NH Xuong, SS Taylor, and JM Sowadski

Department of Chemistry, University of California, San Diego, La Jolla 92093-0654.

The crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase complexed with a 20-amino acid substrate analog inhibitor has been solved and partially refined at 2.7 A resolution to an R factor of 0.212. The magnesium adenosine triphosphate (MgATP) binding site was located by difference Fourier synthesis. The enzyme structure is bilobal with a deep cleft between the lobes. The cleft is filled by MgATP and a portion of the inhibitor peptide. The smaller lobe, consisting mostly of amino-terminal sequence, is associated with nucleotide binding, and its largely antiparallel beta sheet architecture constitutes an unusual nucleotide binding motif. The larger lobe is dominated by helical structure with a single beta sheet at the domain interface. This lobe is primarily involved in peptide binding and catalysis. Residues 40 through 280 constitute a conserved catalytic core that is shared by more than 100 protein kinases. Most of the invariant amino acids in this conserved catalytic core are clustered at the sites of nucleotide binding and catalysis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Homo-oligomerization and Activation of AMP-activated Protein Kinase Are Mediated by the Kinase Domain {alpha}G-Helix.
R. Scholz, M. Suter, T. Weimann, C. Polge, P. V. Konarev, R. F. Thali, R. D. Tuerk, B. Viollet, T. Wallimann, U. Schlattner, et al. (2009)
J. Biol. Chem. 284, 27425-27437
   Abstract »    Full Text »    PDF »
Mechanism of Epac Activation: STRUCTURAL AND FUNCTIONAL ANALYSES OF Epac2 HINGE MUTANTS WITH CONSTITUTIVE AND REDUCED ACTIVITIES.
T. Tsalkova, D. K. Blumenthal, F. C. Mei, M. A. White, and X. Cheng (2009)
J. Biol. Chem. 284, 23644-23651
   Abstract »    Full Text »    PDF »
The PKARI{alpha} Subunit of Protein Kinase A Modulates the Activation of p90RSK1 and Its Function.
D. Chaturvedi, M. S. Cohen, J. Taunton, and T. B. Patel (2009)
J. Biol. Chem. 284, 23670-23681
   Abstract »    Full Text »    PDF »
Distal Recognition Sites in Substrates Are Required for Efficient Phosphorylation by the cAMP-Dependent Protein Kinase.
S. J. Deminoff, V. Ramachandran, and P. K. Herman (2009)
Genetics 182, 529-539
   Abstract »    Full Text »    PDF »
Mapping the conformational transition in Src activation by cumulating the information from multiple molecular dynamics trajectories.
S. Yang, N. K. Banavali, and B. Roux (2009)
PNAS 106, 3776-3781
   Abstract »    Full Text »    PDF »
Contribution of Non-catalytic Core Residues to Activity and Regulation in Protein Kinase A.
J. Yang, E. J. Kennedy, J. Wu, M. S. Deal, J. Pennypacker, G. Ghosh, and S. S. Taylor (2009)
J. Biol. Chem. 284, 6241-6248
   Abstract »    Full Text »    PDF »
Protein Kinase D1 Autophosphorylation via Distinct Mechanisms at Ser744/Ser748 and Ser916.
V. O. Rybin, J. Guo, and S. F. Steinberg (2009)
J. Biol. Chem. 284, 2332-2343
   Abstract »    Full Text »    PDF »
Molecular Mechanisms of HipA-Mediated Multidrug Tolerance and Its Neutralization by HipB.
M. A. Schumacher, K. M. Piro, W. Xu, S. Hansen, K. Lewis, and R. G. Brennan (2009)
Science 323, 396-401
   Abstract »    Full Text »    PDF »
Structural Basis of Protein Kinase C Isoform Function.
S. F. Steinberg (2008)
Physiol Rev 88, 1341-1378
   Abstract »    Full Text »    PDF »
Mechanoenzymatics of titin kinase.
E. M. Puchner, A. Alexandrovich, A. L. Kho, U. Hensen, L. V. Schafer, B. Brandmeier, F. Grater, H. Grubmuller, H. E. Gaub, and M. Gautel (2008)
PNAS 105, 13385-13390
   Abstract »    Full Text »    PDF »
Crystal Structure of the Catalytic Domain of the Mitotic Checkpoint Kinase Mps1 in Complex with SP600125.
M. L. H. Chu, L. M. G. Chavas, K. T. Douglas, P. A. Eyers, and L. Tabernero (2008)
J. Biol. Chem. 283, 21495-21500
   Abstract »    Full Text »    PDF »
Congenital disease SNPs target lineage specific structural elements in protein kinases.
A. Torkamani, N. Kannan, S. S. Taylor, and N. J. Schork (2008)
PNAS 105, 9011-9016
   Abstract »    Full Text »    PDF »
Antitumor Activity and Pharmacology of a Selective Focal Adhesion Kinase Inhibitor, PF-562,271.
W. G. Roberts, E. Ung, P. Whalen, B. Cooper, C. Hulford, C. Autry, D. Richter, E. Emerson, J. Lin, J. Kath, et al. (2008)
Cancer Res. 68, 1935-1944
   Abstract »    Full Text »    PDF »
Allosteric cooperativity in protein kinase A.
L. R. Masterson, A. Mascioni, N. J. Traaseth, S. S. Taylor, and G. Veglia (2008)
PNAS 105, 506-511
   Abstract »    Full Text »    PDF »
WNK1 Is a Novel Regulator of Munc18c-Syntaxin 4 Complex Formation in Soluble NSF Attachment Protein Receptor (SNARE)-mediated Vesicle Exocytosis.
E. Oh, C. J. Heise, J. M. English, M. H. Cobb, and D. C. Thurmond (2007)
J. Biol. Chem. 282, 32613-32622
   Abstract »    Full Text »    PDF »
Regulatory interactions between two actin nucleators, Spire and Cappuccino.
M. E. Quinlan, S. Hilgert, A. Bedrossian, R. D. Mullins, and E. Kerkhoff (2007)
J. Cell Biol. 179, 117-128
   Abstract »    Full Text »    PDF »
cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein.
J. D. Roberts Jr., J.-D. Chiche, E. M. Kolpa, D. B. Bloch, and K. D. Bloch (2007)
Am J Physiol Lung Cell Mol Physiol 293, L903-L912
   Abstract »    Full Text »    PDF »
Mechanistic insight into the dominant mode of the Parkinson's disease-associated G2019S LRRK2 mutation.
B. Luzon-Toro, E. R. de la Torre, A. Delgado, J. Perez-Tur, and S. Hilfiker (2007)
Hum. Mol. Genet. 16, 2031-2039
   Abstract »    Full Text »    PDF »
A novel testis ubiquitin-binding protein gene arose by exon shuffling in hominoids.
D. V. Babushok, K. Ohshima, E. M. Ostertag, X. Chen, Y. Wang, P. K. Mandal, N. Okada, C. S. Abrams, and H. H. Kazazian Jr. (2007)
Genome Res. 17, 1129-1138
   Abstract »    Full Text »    PDF »
From the Cover: Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis.
N. Scherr, S. Honnappa, G. Kunz, P. Mueller, R. Jayachandran, F. Winkler, J. Pieters, and M. O. Steinmetz (2007)
PNAS 104, 12151-12156
   Abstract »    Full Text »    PDF »
c-Abl Tyrosine Kinase and Inhibition by the Cancer Drug Imatinib (Gleevec/STI-571).
B. Nagar (2007)
J. Nutr. 137, 1518S-1523S
   Abstract »    Full Text »    PDF »
The hallmark of AGC kinase functional divergence is its C-terminal tail, a cis-acting regulatory module.
N. Kannan, N. Haste, S. S. Taylor, and A. F. Neuwald (2007)
PNAS 104, 1272-1277
   Abstract »    Full Text »    PDF »
Uses for JNK: the Many and Varied Substrates of the c-Jun N-Terminal Kinases.
M. A. Bogoyevitch and B. Kobe (2006)
Microbiol. Mol. Biol. Rev. 70, 1061-1095
   Abstract »    Full Text »    PDF »
ORF66 Protein Kinase Function Is Required for T-Cell Tropism of Varicella-Zoster Virus In Vivo.
A. Schaap-Nutt, M. Sommer, X. Che, L. Zerboni, and A. M. Arvin (2006)
J. Virol. 80, 11806-11816
   Abstract »    Full Text »    PDF »
Surface comparison of active and inactive protein kinases identifies a conserved activation mechanism.
A. P. Kornev, N. M. Haste, S. S. Taylor, and L. F. Ten Eyck (2006)
PNAS 103, 17783-17788
   Abstract »    Full Text »    PDF »
A Dithio-Coupled Kinase and ATPase Assay.
T. Chiku, P. K. Pullela, and D. S. Sem (2006)
J Biomol Screen 11, 844-853
   Abstract »    PDF »
kinDOCK: a tool for comparative docking of protein kinase ligands..
L. Martin, V. Catherinot, and G. Labesse (2006)
Nucleic Acids Res. 34, W325-W329
   Abstract »    Full Text »    PDF »
The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway.
R. P. Bhattacharyya, A. Remenyi, M. C. Good, C. J. Bashor, A. M. Falick, and W. A. Lim (2006)
Science 311, 822-826
   Abstract »    Full Text »    PDF »
The Conformationally Dynamic C Helix of the RI{alpha} Subunit of Protein Kinase A Mediates Isoform-specific Domain Reorganization upon C Subunit Binding.
D. Vigil, D. K. Blumenthal, S. S. Taylor, and J. Trewhella (2005)
J. Biol. Chem. 280, 35521-35527
   Abstract »    Full Text »    PDF »
Structural Basis for Autoinhibition and Mutational Activation of Eukaryotic Initiation Factor 2{alpha} Protein Kinase GCN2.
A. K. Padyana, H. Qiu, A. Roll-Mecak, A. G. Hinnebusch, and S. K. Burley (2005)
J. Biol. Chem. 280, 29289-29299
   Abstract »    Full Text »    PDF »
Crystal structure of the Jak3 kinase domain in complex with a staurosporine analog.
T. J. Boggon, Y. Li, P. W. Manley, and M. J. Eck (2005)
Blood 106, 996-1002
   Abstract »    Full Text »    PDF »
STRUBBELIG defines a receptor kinase-mediated signaling pathway regulating organ development in Arabidopsis.
D. Chevalier, M. Batoux, L. Fulton, K. Pfister, R. K. Yadav, M. Schellenberg, and K. Schneitz (2005)
PNAS 102, 9074-9079
   Abstract »    Full Text »    PDF »
Role of T-loop Phosphorylation in PDK1 Activation, Stability, and Substrate Binding.
D. Komander, G. Kular, M. Deak, D. R. Alessi, and D. M. F. van Aalten (2005)
J. Biol. Chem. 280, 18797-18802
   Abstract »    Full Text »    PDF »
Crystal Structure of CC3 (TIP30): IMPLICATIONS FOR ITS ROLE AS A TUMOR SUPPRESSOR.
K. El Omari, L. E. Bird, C. E. Nichols, J. Ren, and D. K. Stammers (2005)
J. Biol. Chem. 280, 18229-18236
   Abstract »    Full Text »    PDF »
FLR-4, a Novel Serine/Threonine Protein Kinase, Regulates Defecation Rhythm in Caenorhabditis elegans.
M. Take-uchi, Y. Kobayashi, K. D. Kimura, T. Ishihara, and I. Katsura (2005)
Mol. Biol. Cell 16, 1355-1365
   Abstract »    Full Text »    PDF »
Protein Kinases.
A. Caplan (2005)
Sci. STKE 2005, tr7
   Abstract »    Full Text »    PDF »
Structural Basis of Constitutive Activity and a Unique Nucleotide Binding Mode of Human Pim-1 Kinase.
K. C. Qian, L. Wang, E. R. Hickey, J. Studts, K. Barringer, C. Peng, A. Kronkaitis, J. Li, A. White, S. Mische, et al. (2005)
J. Biol. Chem. 280, 6130-6137
   Abstract »    Full Text »    PDF »
Crystal Structure of a Complex Between the Catalytic and Regulatory (RI{alpha}) Subunits of PKA.
C. Kim, N.-H. Xuong, and S. S. Taylor (2005)
Science 307, 690-696
   Abstract »    Full Text »    PDF »
Catalytic Domain Crystal Structure of Protein Kinase C-{theta} (PKC{theta}).
Z.-B. Xu, D. Chaudhary, S. Olland, S. Wolfrom, R. Czerwinski, K. Malakian, L. Lin, M. L. Stahl, D. Joseph-McCarthy, C. Benander, et al. (2004)
J. Biol. Chem. 279, 50401-50409
   Abstract »    Full Text »    PDF »
Crystal Structures of the Phosphorylated and Unphosphorylated Kinase Domains of the Cdc42-associated Tyrosine Kinase ACK1.
J. C. Lougheed, R.-H. Chen, P. Mak, and T. J. Stout (2004)
J. Biol. Chem. 279, 44039-44045
   Abstract »    Full Text »    PDF »
Ligand Regulates Epidermal Growth Factor Receptor Kinase Specificity: ACTIVATION INCREASES PREFERENCE FOR GAB1 AND SHC VERSUS AUTOPHOSPHORYLATION SITES.
Y.-X. Fan, L. Wong, T. B. Deb, and G. R. Johnson (2004)
J. Biol. Chem. 279, 38143-38150
   Abstract »    Full Text »    PDF »
The Cyclic AMP-Dependent Protein Kinase A Network Regulates Development and Virulence in Aspergillus fumigatus.
B. Liebmann, M. Muller, A. Braun, and A. A. Brakhage (2004)
Infect. Immun. 72, 5193-5203
   Abstract »    Full Text »    PDF »
Characterization of a Eukaryotic-Like Tyrosine Protein Kinase Expressed by the Shiga Toxin-Encoding Bacteriophage 933W.
J. S. Tyler and D. I. Friedman (2004)
J. Bacteriol. 186, 3472-3479
   Abstract »    Full Text »    PDF »
C Subunits Binding to the Protein Kinase A RI{alpha} Dimer Induce a Large Conformational Change.
W. T. Heller, D. Vigil, S. Brown, D. K. Blumenthal, S. S. Taylor, and J. Trewhella (2004)
J. Biol. Chem. 279, 19084-19090
   Abstract »    Full Text »    PDF »
Identification of Critical Residues of Choline Kinase A2 from Caenorhabditis elegans.
C. Yuan and C. Kent (2004)
J. Biol. Chem. 279, 17801-17809
   Abstract »    Full Text »    PDF »
Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met and its complex with the microbial alkaloid K-252a.
N. Schiering, S. Knapp, M. Marconi, M. M. Flocco, J. Cui, R. Perego, L. Rusconi, and C. Cristiani (2003)
PNAS 100, 12654-12659
   Abstract »    Full Text »    PDF »
Communication between the Regulatory and the Catalytic Region of the cAMP-responsive Guanine Nucleotide Exchange Factor Epac.
H. Rehmann, A. Rueppel, J. L. Bos, and A. Wittinghofer (2003)
J. Biol. Chem. 278, 23508-23514
   Abstract »    Full Text »    PDF »
A Single Amino Acid Difference between alpha and beta Ca2+/Calmodulin-dependent Protein Kinase Kinase Dictates Sensitivity to the Specific Inhibitor, STO-609.
H. Tokumitsu, H. Inuzuka, Y. Ishikawa, and R. Kobayashi (2003)
J. Biol. Chem. 278, 10908-10913
   Abstract »    Full Text »    PDF »
Structural Basis for Peptide Binding in Protein Kinase A. ROLE OF GLUTAMIC ACID 203 AND TYROSINE 204 IN THE PEPTIDE-POSITIONING LOOP.
M. J. Moore, J. A. Adams, and S. S. Taylor (2003)
J. Biol. Chem. 278, 10613-10618
   Abstract »    Full Text »    PDF »
Mechanisms associated with cGMP binding and activation of cGMP-dependent protein kinase.
M. E. Wall, S. H. Francis, J. D. Corbin, K. Grimes, R. Richie-Jannetta, J. Kotera, B. A. Macdonald, R. R. Gibson, and J. Trewhella (2003)
PNAS 100, 2380-2385
   Abstract »    Full Text »    PDF »
Targeting Aurora2 Kinase in Oncogenesis: A Structural Bioinformatics Approach to Target Validation and Rational Drug Design.
H. Vankayalapati, D. J. Bearss, J. W. Saldanha, R. M. Munoz, S. Rojanala, D. D. Von Hoff, and D. Mahadevan (2003)
Mol. Cancer Ther. 2, 283-294
   Abstract »    Full Text »    PDF »
Mechanism of Ca2+-mediated Regulation of NDR Protein Kinase through Autophosphorylation and Phosphorylation by an Upstream Kinase.
R. Tamaskovic, S. J. Bichsel, H. Rogniaux, M. R. Stegert, and B. A. Hemmings (2003)
J. Biol. Chem. 278, 6710-6718
   Abstract »    Full Text »    PDF »
From the Cover: Structural basis and prediction of substrate specificity in protein serine/threonine kinases.
R. I. Brinkworth, R. A. Breinl, and B. Kobe (2003)
PNAS 100, 74-79
   Abstract »    Full Text »    PDF »
Regulation of WNK1 by an Autoinhibitory Domain and Autophosphorylation.
B.-e Xu, X. Min, S. Stippec, B.-H. Lee, E. J. Goldsmith, and M. H. Cobb (2002)
J. Biol. Chem. 277, 48456-48462
   Abstract »    Full Text »    PDF »
Phosphorylation of the Catalytic Subunit of Protein Kinase A. AUTOPHOSPHORYLATION VERSUS PHOSPHORYLATION BY PHOSPHOINOSITIDE-DEPENDENT KINASE-1.
M. J. Moore, J. R. Kanter, K.C. Jones, and S. S. Taylor (2002)
J. Biol. Chem. 277, 47878-47884
   Abstract »    Full Text »    PDF »
Biochemical Characterization of the Arabidopsis Protein Kinase SOS2 That Functions in Salt Tolerance.
D. Gong, Y. Guo, A. T. Jagendorf, and J.-K. Zhu (2002)
Plant Physiology 130, 256-264
   Abstract »    Full Text »    PDF »
The Turn Motif Is a Phosphorylation Switch That Regulates the Binding of Hsp70 to Protein Kinase C.
T. Gao and A. C. Newton (2002)
J. Biol. Chem. 277, 31585-31592
   Abstract »    Full Text »    PDF »
Crystal Structures of the Kinase Domain of c-Abl in Complex with the Small Molecule Inhibitors PD173955 and Imatinib (STI-571).
B. Nagar, W. G. Bornmann, P. Pellicena, T. Schindler, D. R. Veach, W. T. Miller, B. Clarkson, and J. Kuriyan (2002)
Cancer Res. 62, 4236-4243
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4-phosphate 5-kinase activity is stimulated during temperature-induced morphogenesis in Candida albicans.
M. L. Hairfield, C. Westwater, and J. W. Dolan (2002)
Microbiology 148, 1737-1746
   Abstract »    Full Text »    PDF »
Toxoplasma gondii Cyclic GMP-Dependent Kinase: Chemotherapeutic Targeting of an Essential Parasite Protein Kinase.
R. G. K. Donald, J. Allocco, S. B. Singh, B. Nare, S. P. Salowe, J. Wiltsie, and P. A. Liberator (2002)
Eukaryot. Cell 1, 317-328
   Abstract »    Full Text »    PDF »
Mutations that bypass tRNA binding activate the intrinsically defective kinase domain in GCN2.
H. Qiu, C. Hu, J. Dong, and A. G. Hinnebusch (2002)
Genes & Dev. 16, 1271-1280
   Abstract »    Full Text »    PDF »
A Structural Model of the Catalytic Subunit-regulatory Subunit Dimeric Complex of the cAMP-dependent Protein Kinase.
C.-S. Tung, D. A. Walsh, and J. Trewhella (2002)
J. Biol. Chem. 277, 12423-12431
   Abstract »    Full Text »    PDF »
Immune System Dysfunction and Autoimmune Disease in Mice Lacking Emk (Par-1) Protein Kinase.
J. B. Hurov, T. S. Stappenbeck, C. M. Zmasek, L. S. White, S. H. Ranganath, J. H. Russell, A. C. Chan, K. M. Murphy, and H. Piwnica-Worms (2001)
Mol. Cell. Biol. 21, 3206-3219
   Abstract »    Full Text »
S and G2 Phase Roles for Cdk2 Revealed by Inducible Expression of a Dominant-Negative Mutant in Human Cells.
B. Hu, J. Mitra, S. van den Heuvel, and G. H. Enders (2001)
Mol. Cell. Biol. 21, 2755-2766
   Abstract »    Full Text »
Cloning and characterization of human haspin gene encoding haploid germ cell-specific nuclear protein kinase.
H. Tanaka, N. Iguchi, Y. Nakamura, J. Kohroki, C. E. de Carvalho, and Y. Nishimune (2001)
Mol. Hum. Reprod. 7, 211-218
   Abstract »    Full Text »    PDF »
Two Distinct Vps34 Phosphatidylinositol 3-Kinase Complexes Function in Autophagy and Carboxypeptidase Y Sorting inSaccharomyces cerevisiae.
A. Kihara, T. Noda, N. Ishihara, and Y. Ohsumi (2001)
J. Cell Biol. 152, 519-530
   Abstract »    Full Text »    PDF »
Simultaneous measurements of intracellular cAMP and L-type Ca2+ current in single frog ventricular myocytes.
J.-M. Goaillard, P. Vincent, and R. Fischmeister (2001)
J. Physiol. 530, 79-91
   Abstract »    Full Text »    PDF »
Guanylyl Cyclases and Signaling by Cyclic GMP.
K. A. Lucas, G. M. Pitari, S. Kazerounian, I. Ruiz-Stewart, J. Park, S. Schulz, K. P. Chepenik, and S. A. Waldman (2000)
Pharmacol. Rev. 52, 375-414
   Abstract »    Full Text »    PDF »
The Unique Catalytic Subunit of Sperm cAMP-dependent Protein Kinase Is the Product of an Alternative Calpha mRNA Expressed Specifically in Spermatogenic Cells.
J. T. S. Agustin, C. G. Wilkerson, and G. B. Witman (2000)
Mol. Biol. Cell 11, 3031-3044
   Abstract »    Full Text »
Characterization of a CREB Gain-of-Function Mutant with Constitutive Transcriptional Activity In Vivo.
K. Du, H. Asahara, U. S. Jhala, B. L. Wagner, and M. Montminy (2000)
Mol. Cell. Biol. 20, 4320-4327
   Abstract »    Full Text »
WNK1, a Novel Mammalian Serine/Threonine Protein Kinase Lacking the Catalytic Lysine in Subdomain II.
B.-e Xu, J. M. English, J. L. Wilsbacher, S. Stippec, E. J. Goldsmith, and M. H. Cobb (2000)
J. Biol. Chem. 275, 16795-16801
   Abstract »    Full Text »    PDF »
Intracellular Distribution of Mammalian Protein Kinase a Catalytic Subunit Altered by Conserved Asn2 Deamidation.
R. Pepperkok, A. Hotz-Wagenblatt, N. Konig, A. Girod, D. Bossemeyer, and V. Kinzel (2000)
J. Cell Biol. 148, 715-726
   Abstract »    Full Text »    PDF »
The C. elegans par-4 gene encodes a putative serine-threonine kinase required for establishing embryonic asymmetry.
J. Watts, D. Morton, J Bestman, and K. Kemphues (2000)
Development 127, 1467-1475
   Abstract »    PDF »
The COOH Terminus of Aminoglycoside Phosphotransferase (3')-IIIa Is Critical for Antibiotic Recognition and Resistance.
P. R. Thompson, J. Schwartzenhauer, D. W. Hughes, A. M. Berghuis, and G. D. Wright (1999)
J. Biol. Chem. 274, 30697-30706
   Abstract »    Full Text »    PDF »
Structural Examination of Autoregulation of Multifunctional Calcium/Calmodulin-dependent Protein Kinase II.
E. Yang and H. Schulman (1999)
J. Biol. Chem. 274, 26199-26208
   Abstract »    Full Text »    PDF »
Regulation of beta -Adrenoceptor Signaling in Cardiac Function and Disease.
N. Dzimiri (1999)
Pharmacol. Rev. 51, 465-502
   Abstract »    Full Text »    PDF »
Peptide Specificity Determinants at P-7 and P-6 Enhance the Catalytic Efficiency of Ca2+/Calmodulin-dependent Protein Kinase I in the Absence of Activation Loop Phosphorylation.
S. S. Hook, B. E. Kemp, and A. R. Means (1999)
J. Biol. Chem. 274, 20215-20222
   Abstract »    Full Text »    PDF »
Phosphorylation of MAP Kinases by MAP/ERK Involves Multiple Regions of MAP Kinases.
J. L. Wilsbacher, E. J. Goldsmith, and M. H. Cobb (1999)
J. Biol. Chem. 274, 16988-16994
   Abstract »    Full Text »    PDF »
Identification and Characterization of a Haploid Germ Cell-specific Nuclear Protein Kinase (Haspin) in Spermatid Nuclei and Its Effects on Somatic Cells.
H. Tanaka, Y. Yoshimura, M. Nozaki, K. Yomogida, J. Tsuchida, Y. Tosaka, T. Habu, T. Nakanishi, M. Okada, H. Nojima, et al. (1999)
J. Biol. Chem. 274, 17049-17057
   Abstract »    Full Text »    PDF »
Substrate Recognition by Ca2+/Calmodulin-dependent Protein Kinase Kinase. ROLE OF THE ARG-PRO-RICH INSERT DOMAIN.
H. Tokumitsu, N. Takahashi, K. Eto, S. Yano, T. R. Soderling, and M.-a. Muramatsu (1999)
J. Biol. Chem. 274, 15803-15810
   Abstract »    Full Text »    PDF »
Dual Specificity Protein Kinase Activity of Testis-specific Protein Kinase 1 and Its Regulation by Autophosphorylation of Serine-215 within the Activation Loop.
J. Toshima, T. Tanaka, and K. Mizuno (1999)
J. Biol. Chem. 274, 12171-12176
   Abstract »    Full Text »    PDF »
Phosphatidylinositol Phosphate Kinases, a Multifaceted Family of Signaling Enzymes.
R. A. Anderson, I. V. Boronenkov, S. D. Doughman, J. Kunz, and J. C. Loijens (1999)
J. Biol. Chem. 274, 9907-9910
   Full Text »    PDF »
Inhibition of the Ca2+/Calmodulin-dependent Protein Kinase I Cascade by cAMP-dependent Protein Kinase.
M. Matsushita and A. C. Nairn (1999)
J. Biol. Chem. 274, 10086-10093
   Abstract »    Full Text »    PDF »
Identification of Substrate Binding Site of Cyclin-dependent Kinase 5.
P. Sharma, P. J. Steinbach, M. Sharma, N. D. Amin, J. J. Barchi Jr., and H. C. Pant (1999)
J. Biol. Chem. 274, 9600-9606
   Abstract »    Full Text »    PDF »
Mitogen-Activated Protein Kinases: Specific Messages from Ubiquitous Messengers.
H. J. Schaeffer and M. J. Weber (1999)
Mol. Cell. Biol. 19, 2435-2444
   Full Text »    PDF »
Effects of Phosphorylation of Threonine 160 on Cyclin-dependent Kinase 2 Structure and Activity.
N. R. Brown, M. E. M. Noble, A. M. Lawrie, M. C. Morris, P. Tunnah, G. Divita, L. N. Johnson, and J. A. Endicott (1999)
J. Biol. Chem. 274, 8746-8756
   Abstract »    Full Text »    PDF »
Carboxyl-terminal Phosphorylation Regulates the Function and Subcellular Localization of Protein Kinase C beta II.
A. S. Edwards, M. C. Faux, J. D. Scott, and A. C. Newton (1999)
J. Biol. Chem. 274, 6461-6468
   Abstract »    Full Text »    PDF »
Structural interpretation of site-directed mutagenesis and specificity of the catalytic subunit of protein kinase CK2 using comparative modelling.
N. Srinivasan, M. Antonelli, G. Jacob, I. Korn, F. Romero, A. Jedlicki, V. Dhanaraj, M. F.-R. Sayed, T. L. Blundell, C. C. Allende, et al. (1999)
Protein Eng. Des. Sel. 12, 119-127
   Abstract »    Full Text »    PDF »
Mutation of a highly conserved aspartate residue in subdomain IX abolishes Fer protein-tyrosine kinase activity.
L.A. Cole, R. Zirngibl, A.W.B. Craig, Z. Jia, and P. Greer (1999)
Protein Eng. Des. Sel. 12, 155-162
   Abstract »    Full Text »    PDF »
The CDK-activating Kinase (Cak1p) from Budding Yeast Has an Unusual ATP-binding Pocket.
D. A. Enke, P. Kaldis, J. K. Holmes, and M. J. Solomon (1999)
J. Biol. Chem. 274, 1949-1956
   Abstract »    Full Text »    PDF »
The Caenorhabditis elegans gene ncc-1 encodes a cdc2-related kinase required for M phase in meiotic and mitotic cell divisions, but not for S phase.
M Boxem, D. Srinivasan, and S van den Heuvel (1999)
Development 126, 2227-2239
   Abstract »    PDF »
Deletion of 11 Amino Acids in p90rsk-mo-1 Abolishes Kinase Activity.
D. J. Spring and E. G. Krebs (1999)
Mol. Cell. Biol. 19, 317-320
   Abstract »    Full Text »    PDF »
Mitogen-Activated Protein Kinase: Conservation of a Three-Kinase Module From Yeast to Human.
C. WIDMANN, S. GIBSON, M. B. JARPE, and G. L. JOHNSON (1999)
Physiol Rev 79, 143-180
   Abstract »    Full Text »    PDF »
Binding of 9-Anthroylcholine Monitors the Interactions of Adenosine Cyclic 3',5'-Phosphate-dependent Protein Kinase with MgATP, Substrates, and Regulatory Subunits.
D. A. Malencik and S. R. Anderson (1998)
J. Biol. Chem. 273, 34049-34056
   Abstract »    Full Text »    PDF »
Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae.
M. D. Mendenhall and A. E. Hodge (1998)
Microbiol. Mol. Biol. Rev. 62, 1191-1243
   Abstract »    Full Text »    PDF »
Intramolecular Regulatory Interactions in the Src Family Kinase Hck Probed by Mutagenesis of a Conserved Tryptophan Residue.
M. LaFevre-Bernt, F. Sicheri, A. Pico, M. Porter, J. Kuriyan, and W. T. Miller (1998)
J. Biol. Chem. 273, 32129-32134
   Abstract »    Full Text »    PDF »
Quaternary Structures of a Catalytic Subunit-Regulatory Subunit Dimeric Complex and the Holoenzyme of the cAMP-dependent Protein Kinase by Neutron Contrast Variation.
J. Zhao, E. Hoye, S. Boylan, D. A. Walsh, and J. Trewhella (1998)
J. Biol. Chem. 273, 30448-30459
   Abstract »    Full Text »    PDF »
Characterization of Functional Regions in the Schizosaccharomyces pombe mei3 Developmental Activator.
W. Wang, P. Li, A. Schettino, Z. Peng, and M. McLeod (1998)
Genetics 150, 1007-1018
   Abstract »    Full Text »
Cleavage and Activation of p21-activated Protein Kinase gamma -PAK by CPP32 (Caspase 3). EFFECTS OF AUTOPHOSPHORYLATION ON ACTIVITY.
B. N. Walter, Z. Huang, R. Jakobi, P. T. Tuazon, E. S. Alnemri, G. Litwack, and J. A. Traugh (1998)
J. Biol. Chem. 273, 28733-28739
   Abstract »    Full Text »    PDF »
Dissecting cAMP Binding Domain A in the RIalpha Subunit of cAMP-dependent Protein Kinase. DISTINCT SUBSITES FOR RECOGNITION OF cAMP AND THE CATALYTIC SUBUNIT.
L. J.-s. Huang and S. S. Taylor (1998)
J. Biol. Chem. 273, 26739-26746
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)