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Science 19 July 1991:
Vol. 253. no. 5017, pp. 320 - 323
DOI: 10.1126/science.1857969
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Articles

Science, Vol 253, Issue 5017, 320-323
Copyright © 1991 by American Association for the Advancement of Science

articles

Inhibition of entry of HIV-1 in neural cell lines by antibodies against galactosyl ceramide

JM Harouse, S Bhat, SL Spitalnik, M Laughlin, K Stefano, DH Silberberg, and F Gonzalez-Scarano

Department of Neurology, University of Pennsylvania Medical Center, Philadelphia 19104.

Although the CD4 molecule is the principal cellular receptor for the human immunodeficiency virus (HIV), several CD4-negative cell lines are susceptible to infection with one or more HIV strains. These findings indicate that there are alternate modes of viral entry, perhaps involving one or more receptor molecules. Antibodies against galactosyl ceramide (galactocerebroside, or GalC) inhibited viral internalization and infection in two CD4-negative cell lines derived from the nervous system: U373-MG and SK-N-MC. Furthermore, recombinant HIV surface glycoprotein gp120 bound to GalC but not to other glycolipids. These results suggest a role for GalC or a highly related molecule in HIV entry into neural cells.


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