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Science 12 July 1991:
Vol. 253. no. 5016, pp. 202 - 205
DOI: 10.1126/science.1712984
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Articles

Science, Vol 253, Issue 5016, 202-205
Copyright © 1991 by American Association for the Advancement of Science

articles

Demonstration that CFTR is a chloride channel by alteration of its anion selectivity

MP Anderson, RJ Gregory, S Thompson, DW Souza, S Paul, RC Mulligan, AE Smith, and MJ Welsh

Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.

Expression of the cystic fibrosis transmembrane conductance regulator (CFTR) generates adenosine 3',5'-monophosphate (cAMP)-regulated chloride channels, indicating that CFTR is either a chloride channel or a chloride channel regulator. To distinguish between these possibilities, basic amino acids in the putative transmembrane domains were mutated. The sequence of anion selectivity of cAMP-regulated channels in cells containing either endogenous or recombinant CFTR was bromide greater than chloride greater than iodide greater than fluoride. Mutation of the lysines at positions 95 or 335 to acidic amino acids converted the selectivity sequence to iodide greater than bromide greater than chloride greater than fluoride. These data indicate that CFTR is a cAMP-regulated chloride channel and that lysines 95 and 335 determine anion selectivity.


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Am J Physiol Lung Cell Mol Physiol 275, L1095-L1099
   Abstract »    Full Text »    PDF »
Covalent Modification of the Nucleotide Binding Domains of Cystic Fibrosis Transmembrane Conductance Regulator.
J. F. Cotten and M. J. Welsh (1998)
J. Biol. Chem. 273, 31873-31879
   Abstract »    Full Text »    PDF »
The Second Half of the Cystic Fibrosis Transmembrane Conductance Regulator Forms a Functional Chloride Channel.
S. Devidas, H. Yue, and W. B. Guggino (1998)
J. Biol. Chem. 273, 29373-29380
   Abstract »    Full Text »    PDF »
Cystic Fibrosis Transmembrane Conductance Regulator-associated ATP Release Is Controlled by a Chloride Sensor.
Q. Jiang, D. Mak, S. Devidas, E. M. Schwiebert, A. Bragin, Y. Zhang, W. R. Skach, W. B. Guggino, J. K. Foskett, and J. F. Engelhardt (1998)
J. Cell Biol. 143, 645-657
   Abstract »    Full Text »    PDF »
Non-pore lining amino acid side chains influence anion selectivity of the human CFTR Cl- channel expressed in mammalian cell lines.
P. Linsdell, S.-X. Zheng, and J. W Hanrahan (1998)
J. Physiol. 512, 1-16
   Abstract »    Full Text »    PDF »
Focus on "Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial cells".
W. B. Guggino (1998)
Am J Physiol Cell Physiol 275, C911-C912
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Characterization of an Allele-Nonspecific Intragenic Suppressor in the Yeast Plasma Membrane H+-ATPase Gene (PMA1).
A. M. Maldonado, N. de la Fuente, and F. Portillo (1998)
Genetics 150, 11-19
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Membrane Trafficking of the Cystic Fibrosis Gene Product, Cystic Fibrosis Transmembrane Conductance Regulator, Tagged with Green Fluorescent Protein in Madin-Darby Canine Kidney Cells.
B. D. Moyer, J. Loffing, E. M. Schwiebert, D. Loffing-Cueni, P. A. Halpin, K. H. Karlson, I. I. Ismailov, W. B. Guggino, G. M. Langford, and B. A. Stanton (1998)
J. Biol. Chem. 273, 21759-21768
   Abstract »    Full Text »    PDF »
Partial restoration of cAMP-stimulated CFTR chloride channel activity in Delta F508 cells by deoxyspergualin.
C. Jiang, S. L. Fang, Y.-F. Xiao, S. P. O'Connor, S. G. Nadler, D. W. Lee, D. M. Jefferson, J. M. Kaplan, A. E. Smith, and S. H. Cheng (1998)
Am J Physiol Cell Physiol 275, C171-C178
   Abstract »    Full Text »    PDF »
Expression of a hypotonic swelling-activated Cl conductance during ontogeny of collecting duct epithelium.
S. M. Huber and M. F. Horster (1998)
Am J Physiol Renal Physiol 275, F25-F32
   Abstract »    Full Text »    PDF »
Cl- transport by cystic fibrosis transmembrane conductance regulator (CFTR) contributes to the inhibition of epithelial Na+ channels (ENaCs) in Xenopus oocytes co-expressing CFTR and ENaC.
M Briel, R Greger, and K Kunzelmann (1998)
J. Physiol. 508, 825-836
   Abstract »    Full Text »    PDF »
Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E2.
C Deachapunya and S M O'Grady (1998)
J. Physiol. 508, 31-47
   Abstract »    Full Text »    PDF »
Sertoli cell expression of the cystic fibrosis transmembrane conductance regulator.
F. R. Boockfor, R. A. Morris, D. C. DeSimone, D. M. Hunt, and K. B. Walsh (1998)
Am J Physiol Cell Physiol 274, C922-C930
   Abstract »    Full Text »    PDF »
Arylaminobenzoate Block of the Cardiac Cyclic AMP-Dependent Chloride Current.
K. B. Walsh and C. Wang (1998)
Mol. Pharmacol. 53, 539-546
   Abstract »    Full Text »


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