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Science 5 July 1991:
Vol. 253. no. 5015, pp. 74 - 76
DOI: 10.1126/science.1829549
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Articles

Science, Vol 253, Issue 5015, 74-76
Copyright © 1991 by American Association for the Advancement of Science

articles

The ras oncoprotein and M-phase activity

I Daar, AR Nebreda, N Yew, P Sass, R Paules, E Santos, M Wigler, and GF Vande Woude

ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21701.

The endogenous mos proto-oncogene product (Mos) is required for meiotic maturation. In Xenopus oocytes, the ras oncogene product (Ras) can induce meiotic maturation and high levels of M-phase--promoting factor (MPF) independent of endogenous Mos, indicating that a parallel pathway to metaphase exists. In addition, Ras, like Mos and cytostatic factor, can arrest Xenopus embryonic cell cleavage in mitosis and maintain high levels of MPF. Thus, in the Xenopus oocyte and embryo systems Ras functions in the M phase of the cell cycle. The embryonic cleavage arrest assay is a rapid and sensitive test for Ras function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mechanisms Regulating Oocyte Meiotic Resumption: Roles of Mitogen-Activated Protein Kinase.
C.-G. Liang, Y.-Q. Su, H.-Y. Fan, H. Schatten, and Q.-Y. Sun (2007)
Mol. Endocrinol. 21, 2037-2055
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Activation of the p42 Mitogen-activated Protein Kinase Pathway Inhibits Cdc2 Activation and Entry into M-Phase in Cycling Xenopus Egg Extracts.
J. C. Bitangcol, A. S.-S. Chau, E. Stadnick, M. J. Lohka, B. Dicken, and E. K. Shibuya (1998)
Mol. Biol. Cell 9, 451-467
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Cleavage Arrest of Early Frog Embryos by the G Protein-activated Protein Kinase PAK I.
R. D. Rooney, P. T. Tuazon, W. E. Meek, E. J. Carroll Jr., J. J. Hagen, E. L. Gump, C. A. Monnig, T. Lugo, and J. A. Traugh (1996)
J. Biol. Chem. 271, 21498-21504
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Requirement of Saccharomyces cerevisiae Ras for Completion of Mitosis.
T. Morishita, H. Mitsuzawa, M. Nakafuku, S. Nakamura, S. Hattori, and Y. Anraku (1995)
Science 270, 1213-1215
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Raf-1 Is Activated during Mitosis.
A. D. Laird, S. J. Taylor, M. Oberst, and D. Shalloway (1995)
J. Biol. Chem. 270, 26742-26745
   Abstract »    Full Text »    PDF »
Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase.
O Haccard, B Sarcevic, A Lewellyn, R Hartley, L Roy, T Izumi, E Erikson, and J. Maller (1993)
Science 262, 1262-1265
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