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Science 21 June 1991:
Vol. 252. no. 5013, pp. 1643 - 1651
DOI: 10.1126/science.2047872

Articles

Science, Vol 252, Issue 5013, 1643-1651
Copyright © 1991 by American Association for the Advancement of Science


articles

Recent advances in the polymerase chain reaction

HA Erlich, D Gelfand, and JJ Sninsky

Department of Human Genetics, Core Technology, Cetus Corporation, Emeryville, CA 94608.

The polymerase chain reaction (PCR) has dramatically altered how molecular studies are conducted as well as what questions can be asked. In addition to simplifying molecular tasks typically carried out with the use of recombinant DNA technology, PCR has allowed a spectrum of advances ranging from the identification of novel genes and pathogens to the quantitation of characterized nucleotide sequences. PCR can provide insights into the intricacies of single cells as well as the evolution of species. Some recent developments in instrumentation, methodology, and applications of the PCR are presented in this review.


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