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Science 14 June 1991:
Vol. 252. no. 5012, pp. 1553 - 1556
DOI: 10.1126/science.1904626
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Articles

Science, Vol 252, Issue 5012, 1553-1556
Copyright © 1991 by American Association for the Advancement of Science

articles

Mediation of the attachment or fusion step in vesicular transport by the GTP-binding Ypt1 protein

N Segev

Department of Pharmacological and Physiological Sciences, University of Chicago, IL 60637.

The function of the guanosine triphosphate (GTP)-binding protein Ypt1 in regulating vesicular traffic was studied in a cell-free system that reconstitutes transport from the endoplasmic reticulum to the Golgi. Blocking the Ypt1 protein activity resulted in accumulation of vesicles that act as an intermediate passing between the two compartments. The Ypt1 protein was found on the outer side of these vesicles. The transport process is completed by fusion of these vesicles with the acceptor compartment, and Ypt1 protein activity was needed for this step. Thus, a specific GTP-binding protein is required for either attachment or fusion (or both) of secretory vesicles with the acceptor compartment during protein secretion.


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