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Science 14 June 1991:
Vol. 252. no. 5012, pp. 1544 - 1546
DOI: 10.1126/science.2047860
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Articles

Science, Vol 252, Issue 5012, 1544-1546
Copyright © 1991 by American Association for the Advancement of Science

articles

Monolayer crystallization of flagellar L-P rings by sequential addition and depletion of lipid

T Akiba, H Yoshimura, and K Namba

ERATO, Molecular Dynamic Assembly Project, Tsukuba, Japan.

The L-P ring complex is thought to be a molecular bushing that supports flagellar motor rotation at about 10,000 revolutions per minute with presumably very little friction. Structural studies of this complex have been limited because only very small amount of samples are available. Therefore devising an efficient method of crystallization was essential. The addition of a phospholipid and its subsequent slow depletion by phospholipase A2 have been used to successfully grow well-ordered monolayer crystals that extend up to about 10 micrometers. The interaction of the L-P ring complex with lipid membranes was also visualized during this process.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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