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Science 10 May 1991:
Vol. 252. no. 5007, pp. 842 - 844
DOI: 10.1126/science.1827531
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Articles

Science, Vol 252, Issue 5007, 842-844
Copyright © 1991 by American Association for the Advancement of Science

articles

HBV X protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions

HF Maguire, JP Hoeffler, and A Siddiqui

Department of Microbiology and Immunology, University of Colorado School of Medicine, Denver 80262.

The hepatitis B virus (HBV) X gene product trans-activates viral and cellular genes. The X protein (pX) does not bind independently to nucleic acids. The data presented here demonstrate that pX entered into a protein-protein complex with the cellular transcriptional factors CREB and ATF-2 and altered their DNA binding specificities. Although CREB and ATF-2 alone did not bind to the HBV enhancer element, a pX-CREB or pX-ATF-2 complex did bind to the HBV enhancer. Thus, the ability of pX to interact with cellular factors broadened the DNA binding specificity of these regulatory proteins and provides a mechanism for pX to participate in transcriptional regulation. This strategy of altered binding specificity may modify the repertoire of genes that can be regulated by transcriptional factors during viral infection.


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J. Biol. Chem. 273, 27097-27103
   Abstract »    Full Text »    PDF »
Liver-specific Enhancer II Is the Target for the p53-mediated Inhibition of Hepatitis B Viral Gene Expression.
H. Lee, H. T. Kim, and Y. Yun (1998)
J. Biol. Chem. 273, 19786-19791
   Abstract »    Full Text »    PDF »
The Human T-cell Leukemia Virus-1 Transcriptional Activator Tax Enhances cAMP-responsive Element-binding Protein (CREB) Binding Activity through Interactions with the DNA Minor Groove.
J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. S. Huang, J. P. Richards, R. G. Brennan, and R. H. Goodman (1998)
J. Biol. Chem. 273, 19251-19259
   Abstract »    Full Text »    PDF »
Immune Pathogenesis of Hepatocellular Carcinoma.
Y. Nakamoto, L. G. Guidotti, C. V. Kuhlen, P. Fowler, and F. V. Chisari (1998)
J. Exp. Med. 188, 341-350
   Abstract »    Full Text »    PDF »
pX, the HBV-encoded coactivator, suppresses the phenotypes of TBP and TAFII250 mutants.
I. Haviv, Y. Matza, and Y. Shaul (1998)
Genes & Dev. 12, 1217-1226
   Abstract »    Full Text »
Cloning and Characterization of a Novel Hepatitis B Virus x Binding Protein That Inhibits Viral Replication.
M. Melegari, P. P. Scaglioni, and J. R. Wands (1998)
J. Virol. 72, 1737-1743
   Abstract »    Full Text »    PDF »
Hepatitis B Virus pX Targets TFIIB in Transcription Coactivation.
I. Haviv, M. Shamay, G. Doitsh, and Y. Shaul (1998)
Mol. Cell. Biol. 18, 1562-1569
   Abstract »    Full Text »
Hepatitis B Virus X Protein Interferes with Cellular DNA Repair.
S. A. Becker, T.-H. Lee, J. S. Butel, and B. L. Slagle (1998)
J. Virol. 72, 266-272
   Abstract »    Full Text »    PDF »
Hepatitis B virus X protein and p53 tumor suppressor interactions in the modulation of apoptosis.
L. W. Elmore, A. R. Hancock, S.-F. Chang, X. W. Wang, S. Chang, C. P. Callahan, D. A. Geller, H. Will, and C. C. Harris (1997)
PNAS 94, 14707-14712
   Abstract »    Full Text »    PDF »
The Hepatitis B Virus X Protein Enhances the DNA Binding Potential and Transcription Efficacy of bZip Transcription Factors.
S. Barnabas, T. Hai, and O. M. Andrisani (1997)
J. Biol. Chem. 272, 20684-20690
   Abstract »    Full Text »    PDF »
Hepatitis B virus HBx protein sensitizes cells to apoptotic killing by tumor necrosis factor alpha.
F. Su and R. J. Schneider (1997)
PNAS 94, 8744-8749
   Abstract »    Full Text »    PDF »
The hepatitis B virus X gene induces p53-mediated programmed cell death.
P. Chirillo, S. Pagano, G. Natoli, P. L. Puri, V. L. Burgio, C. Balsano, and M. Levrero (1997)
PNAS 94, 8162-8167
   Abstract »    Full Text »    PDF »
Activating Transcription Factor 2 (ATF2) Down-regulates Hepatitis B Virus X Promoter Activity by the Competition for the Activating Protein 1 Binding Site and the Formation of the ATF2-Jun Heterodimer.
C. Y. Choi, B. H. Choi, G. T. Park, and H. M. Rho (1997)
J. Biol. Chem. 272, 16934-16939
   Abstract »    Full Text »    PDF »
The Hepatitis B Virus X-associated Protein, XAP3, Is a Protein Kinase C-binding Protein.
Y.-S. Cong, Y.-L. Yao, W.-M. Yang, N. Kuzhandaivelu, and E. Seto (1997)
J. Biol. Chem. 272, 16482-16489
   Abstract »    Full Text »    PDF »
Hepatitis B Virus X Protein Is a Transcriptional Modulator That Communicates with Transcription Factor IIB and the RNA Polymerase II Subunit 5.
Y. Lin, T. Nomura, J. Cheong, D. Dorjsuren, K. Iida, and S. Murakami (1997)
J. Biol. Chem. 272, 7132-7139
   Abstract »    Full Text »    PDF »
Identification of the cAMP Response Element That Controls Transcriptional Activation of the Insulin-like Growth Factor-I Gene by Prostaglandin E2 in Osteoblasts.
M. J. Thomas, Y. Umayahara, H. Shu, M. Centrella, P. Rotwein, and T. L. McCarthy (1996)
J. Biol. Chem. 271, 21835-21841
   Abstract »    Full Text »    PDF »
Purification and Properties of Rat Liver Nuclear Proteins That Interact with the Hepatitis B Virus Enhancer 1.
M. J. Kosovsky, B. Huan, and A. Siddiqui (1996)
J. Biol. Chem. 271, 21859-21869
   Abstract »    Full Text »    PDF »
The Hepatitis B Virus Transactivator Protein, HBx, Interacts with Single-stranded DNA (ssDNA). BIOCHEMICAL CHARACTERIZATIONS OF THE HBx-ssDNA INTERACTIONS.
I. Qadri, M. E. Ferrari, and A. Siddiqui (1996)
J. Biol. Chem. 271, 15443-15450
   Abstract »    Full Text »    PDF »
Intramolecular inhibition of activating transcription factor-2 function by its DNA-binding domain..
X Y Li and M R Green (1996)
Genes & Dev. 10, 517-527
   Abstract »    PDF »
Transcription factor ATF2 regulation by the JNK signal transduction pathway.
S Gupta, D Campbell, B Derijard, and R. Davis (1995)
Science 267, 389-393
   Abstract »    PDF »
Large Isoform of Hepatitis Delta Antigen Activates Serum Response Factor-associated Transcription.
T. Goto, N. Kato, S. K. Ono-Nita, H. Yoshida, M. Otsuka, Y. Shiratori, and M. Omata (2000)
J. Biol. Chem. 275, 37311-37316
   Abstract »    Full Text »    PDF »
The Hepatitis B Virus X Protein Induces HIV-1 Replication and Transcription in Synergy with T-cell Activation Signals. FUNCTIONAL ROLES OF NF-kappa B/NF-AT AND SP1-BINDING SITES IN THE HIV-1 LONG TERMINAL REPEAT PROMOTER.
M. Gomez-Gonzalo, M. Carretero, J. Rullas, E. Lara-Pezzi, J. Aramburu, B. Berkhout, J. Alcami, and M. Lopez-Cabrera (2001)
J. Biol. Chem. 276, 35435-35443
   Abstract »    Full Text »    PDF »
Hepatitis B Virus X Protein Differentially Activates RAS-RAF-MAPK and JNK Pathways in X-transforming Versus Non-transforming AML12 Hepatocytes.
C. Tarn, S. Lee, Y. Hu, C. Ashendel, and O. M. Andrisani (2001)
J. Biol. Chem. 276, 34671-34680
   Abstract »    Full Text »    PDF »
Structural and Functional Characterization of Interaction between Hepatitis B Virus X Protein and the Proteasome Complex.
Z. Zhang, N. Torii, A. Furusaka, N. Malayaman, Z. Hu, and T. J. Liang (2000)
J. Biol. Chem. 275, 15157-15165
   Abstract »    Full Text »    PDF »


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