Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 May 1991:
Vol. 252. no. 5006, pp. 698 - 702
DOI: 10.1126/science.1902591
Prev | Table of Contents | Next

Articles

Science, Vol 252, Issue 5006, 698-702
Copyright © 1991 by American Association for the Advancement of Science

articles

Three-dimensional structure of recombinant human interferon-gamma

SE Ealick, WJ Cook, S Vijay-Kumar, M Carson, TL Nagabhushan, PP Trotta, and CE Bugg

Department of Pharmacology, University of Alabama, Birmingham 35294.

The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phylogeny and evolution of class-I helical cytokines..
M. O Huising, C. P Kruiswijk, and G. Flik (2006)
J. Endocrinol. 189, 1-25
   Abstract »    Full Text »    PDF »
Identification and Bioactivities of IFN-{gamma} in Rainbow Trout Oncorhynchus mykiss: The First Th1-Type Cytokine Characterized Functionally in Fish.
J. Zou, A. Carrington, B. Collet, J. M. Dijkstra, Y. Yoshiura, N. Bols, and C. Secombes (2005)
J. Immunol. 175, 2484-2494
   Abstract »    Full Text »    PDF »
Mechanistic Diversity of Cytokine Receptor Signaling Across Cell Membranes.
R. M. Stroud and J. A. Wells (2004)
Sci. STKE 2004, re7
   Abstract »    Full Text »    PDF »
Interferon-{gamma}: an overview of signals, mechanisms and functions.
K. Schroder, P. J. Hertzog, T. Ravasi, and D. A. Hume (2004)
J. Leukoc. Biol. 75, 163-189
   Abstract »    Full Text »    PDF »
Glycation and Post-translational Processing of Human Interferon-{gamma} Expressed in Escherichia coli.
R. Mironova, T. Niwa, R. Dimitrova, M. Boyanova, and I. Ivanov (2003)
J. Biol. Chem. 278, 51068-51074
   Abstract »    Full Text »    PDF »
Transfected Plasmodium knowlesi Produces Bioactive Host Gamma Interferon: a New Perspective for Modulating Immune Responses to Malaria Parasites.
H. Ozwara, J. A. M. Langermans, C. H. M. Kocken, A. van der Wel, P. H. van der Meide, R. A. W. Vervenne, J. M. Mwenda, and A. W. Thomas (2003)
Infect. Immun. 71, 4375-4381
   Abstract »    Full Text »    PDF »
Interleukin-10 Therapy--Review of a New Approach.
K. Asadullah, W. Sterry, and H. D. Volk (2003)
Pharmacol. Rev. 55, 241-269
   Abstract »    Full Text »    PDF »
Crystal Structure of Interleukin-19 Defines a New Subfamily of Helical Cytokines.
C. Chang, E. Magracheva, S. Kozlov, S. Fong, G. Tobin, S. Kotenko, A. Wlodawer, and A. Zdanov (2003)
J. Biol. Chem. 278, 3308-3313
   Abstract »    Full Text »    PDF »
Transport of Exogenous Growth Factors and Cytokines to the Cytosol and to the Nucleus.
S. Olsnes, O. Klingenberg, and A. Wiedlocha (2003)
Physiol Rev 83, 163-182
   Abstract »    Full Text »    PDF »
A study of the vaccinia virus interferon-{gamma} receptor and its contribution to virus virulence.
J. A. Symons, D. C. Tscharke, N. Price, and G. L. Smith (2002)
J. Gen. Virol. 83, 1953-1964
   Abstract »    Full Text »    PDF »
The Conserved Helix C Region in the Superfamily of Interferon-gamma /Interleukin-10-related Cytokines Corresponds to a High-affinity Binding Site for the HSP70 Chaperone DnaK.
K. Vandenbroeck, I. Alloza, D. Brehmer, A. Billiau, P. Proost, N. McFerran, S. Rudiger, and B. Walker (2002)
J. Biol. Chem. 277, 25668-25676
   Abstract »    Full Text »    PDF »
Orf virus-encoded interleukin-10 stimulates the proliferation of murine mast cells and inhibits cytokine synthesis in murine peritoneal macrophages.
W. Imlach, C. A. McCaughan, A. A. Mercer, D. Haig, and S. B. Fleming (2002)
J. Gen. Virol. 83, 1049-1058
   Abstract »    Full Text »    PDF »
The vaccinia virus soluble interferon-{gamma} receptor is a homodimer.
A. Alcami and G. L. Smith (2002)
J. Gen. Virol. 83, 545-549
   Abstract »    Full Text »    PDF »
Human cytomegalovirus harbors its own unique IL-10 homolog (cmvIL-10).
S. V. Kotenko, S. Saccani, L. S. Izotova, O. V. Mirochnitchenko, and S. Pestka (2000)
PNAS 97, 1695-1700
   Abstract »    Full Text »    PDF »
CD44, a Cell Surface Chondroitin Sulfate Proteoglycan, Mediates Binding of Interferon-gamma and Some of Its Biological Effects on Human Vascular Smooth Muscle Cells.
E. Hurt-Camejo, B. Rosengren, P. Sartipy, K. Elfsberg, G. Camejo, and L. Svensson (1999)
J. Biol. Chem. 274, 18957-18964
   Abstract »    Full Text »    PDF »
Increased Antimycobacterial Immunity in Interleukin-10-Deficient Mice.
P. J. Murray and R. A. Young (1999)
Infect. Immun. 67, 3087-3095
   Abstract »    Full Text »    PDF »
The Heparan Sulfate Binding Sequence of Interferon-gamma Increased the On Rate of the Interferon-gamma -Interferon-gamma Receptor Complex Formation.
R. Sadir, E. Forest, and H. Lortat-Jacob (1998)
J. Biol. Chem. 273, 10919-10925
   Abstract »    Full Text »    PDF »
Interferon-gamma -Inhibitory Oligodeoxynucleotides Alter the Conformation of Interferon-gamma.
V. Balasubramanian, L. T. Nguyen, S. V. Balasubramanian, and M. Ramanathan (1998)
Mol. Pharmacol. 53, 926-932
   Abstract »    Full Text »
The crystal structure of human interferon beta  at 2.2-A resolution.
M. Karpusas, M. Nolte, C. B. Benton, W. Meier, W. N. Lipscomb, and S. Goelz (1997)
PNAS 94, 11813-11818
   Abstract »    Full Text »    PDF »
Chimeric Erythropoietin-Interferon gamma Receptors Reveal Differences in Functional Architecture of Intracellular Domains for Signal Transduction.
G. Muthukumaran, S. Kotenko, R. Donnelly, J. N. Ihle, and S. Pestka (1997)
J. Biol. Chem. 272, 4993-4999
   Abstract »    Full Text »    PDF »
Interferon Gamma Binds to Extracellular Matrix Chondroitin-Sulfate Proteoglycans, Thus Enhancing Its Cellular Response.
E. H. Camejo, B. Rosengren, G. Camejo, P. Sartipy, G. Fager, and G. Bondjers (1995)
Arterioscler. Thromb. Vasc. Biol. 15, 1456-1465
   Abstract »    Full Text »
Development of a Receptor Peptide Antagonist to Human [IMAGE]-Interferon and Characterization of Its Ligand-bound Conformation Using Transferred Nuclear Overhauser Effect Spectroscopy.
G. F. Seelig, W. W. Prosise, J. C. Hawkins, and M. M. Senior (1995)
J. Biol. Chem. 270, 9241-9249
   Abstract »    Full Text »    PDF »
Interferon [IMAGE] Receptor Extracellular Domain Expressed as IgG Fusion Protein in Chinese Hamster Ovary Cells.
M. Fountoulakis, C. Mesa, G. Schmid, R. Gentz, M. Manneberg, M. Zulauf, Z. Dembic, and G. Garotta (1995)
J. Biol. Chem. 270, 3958-3964
   Abstract »    Full Text »    PDF »
Crystal structure of a synthetic triple-stranded alpha-helical bundle.
B Lovejoy, S Choe, D Cascio, D. McRorie, W. DeGrado, and D Eisenberg (1993)
Science 259, 1288-1293
   Abstract »    PDF »
Ordered self-assembly of polypeptide fragments to form nativelike dimeric trp repressor.
M. Tasayco and J Carey (1992)
Science 255, 594-597
   Abstract »    PDF »
Partial molar volume, surface area, and hydration changes for equilibrium unfolding and formation of aggregation transition state: High-pressure and cosolute studies on recombinant human IFN-gamma.
J. N. Webb, S. D. Webb, J. L. Cleland, J. F. Carpenter, and T. W. Randolph (2001)
PNAS 98, 7259-7264
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)