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Science 3 May 1991:
Vol. 252. no. 5006, pp. 691 - 693
DOI: 10.1126/science.2024121
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Articles

Science, Vol 252, Issue 5006, 691-693
Copyright © 1991 by American Association for the Advancement of Science

articles

Three-dimensional readout of flash x-ray images of living sperm in water by atomic-force microscopy

T Tomie, H Shimizu, T Majima, M Yamada, T Kanayama, H Kondo, M Yano, and M Ono

Electrotechnical Laboratory, Ibaraki, Japan.

The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond [corrected] x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.


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