Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 26 April 1991:
Vol. 252. no. 5005, pp. 561 - 563
DOI: 10.1126/science.1850551
Prev | Table of Contents | Next

Articles

Science, Vol 252, Issue 5005, 561-563
Copyright © 1991 by American Association for the Advancement of Science

articles

Chimeric NGF-EGF receptors define domains responsible for neuronal differentiation

H Yan, J Schlessinger, and MV Chao

Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.

To determine the domains of the low-affinity nerve growth factor (NGF) receptor required for appropriate signal transduction, a series of hybrid receptors were constructed that consisted of the extracellular ligand-binding domain of the human epidermal growth factor (EGF) receptor (EGFR) fused to the transmembrane and cytoplasmic domains of the human low-affinity NGF receptor (NGFR). Transfection of these chimeric receptors into rat pheochromocytoma PC12 cells resulted in appropriate cell surface expression. Biological activity mediated by the EGF-NGF chimeric receptor was assayed by the induction of neurite outgrowth in response to EGF in stably transfected cells. Furthermore, the chimeric receptor mediated nuclear signaling, as evidenced by the specific induction of transin messenger RNA, an NGF-responsive gene. Neurite outgrowth was not observed with chimeric receptors that contained the transmembrane domain from the EGFR, suggesting that the membrane-spanning region and cytoplasmic domain of the low-affinity NGFR are necessary for signal transduction.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Ligand-inducible Epidermal Growth Factor Receptor/Anaplastic Lymphoma Kinase Chimera Promotes Mitogenesis and Transforming Properties in 3T3 Cells.
G. Piccinini, R. Bacchiocchi, M. Serresi, C. Vivani, S. Rossetti, C. Gennaretti, D. Carbonari, and F. Fazioli (2002)
J. Biol. Chem. 277, 22231-22239
   Abstract »    Full Text »    PDF »
Protein 4.1N Binding to Nuclear Mitotic Apparatus Protein in PC12 Cells Mediates the Antiproliferative Actions of Nerve Growth Factor.
K. Ye, D. A. Compton, M. M. Lai, L. D. Walensky, and S. H. Snyder (1999)
J. Neurosci. 19, 10747-10756
   Abstract »    Full Text »    PDF »
EGFR signaling is required for the differentiation and maintenance of neural progenitors along the dorsal midline of the Drosophila embryonic head.
K Dumstrei, C Nassif, G Abboud, A Aryai, A Aryai, and V Hartenstein (1998)
Development 125, 3417-3426
   Abstract »    PDF »
A Dominant Role of the Juxtamembrane Region of the TrkA Nerve Growth Factor Receptor during Neuronal Cell Differentiation.
S. O. Yoon, S. P. Soltoff, and M. V. Chao (1997)
J. Biol. Chem. 272, 23231-23238
   Abstract »    Full Text »    PDF »
CSF-1 Receptor/Insulin Receptor Chimera Permits CSF-1-dependent Differentiation of 3T3-L1 Preadipocytes.
O. V. Chaika, N. Chaika, D. J. Volle, P. A. Wilden, S. J. Pirrucello, and R. E. Lewis (1997)
J. Biol. Chem. 272, 11968-11974
   Abstract »    Full Text »    PDF »
Agonist-stimulated Ligand Binding by the Platelet Integrin alphaIIbbeta3 in a Lymphocyte Expression System.
E. Loh, K. Beaverson, G. Vilaire, W. Qi, M. Poncz, and J. S. Bennett (1995)
J. Biol. Chem. 270, 18631-18636
   Abstract »    Full Text »    PDF »
The role of yan in mediating the choice between cell division and differentiation.
R Rogge, P. Green, J Urano, S Horn-Saban, M Mlodzik, B. Shilo, V Hartenstein, and U Banerjee (1995)
Development 121, 3947-3958
   Abstract »    PDF »
Activation of the sphingomyelin cycle through the low-affinity neurotrophin receptor.
R. Dobrowsky, M. Werner, A. Castellino, M. Chao, and Y. Hannun (1994)
Science 265, 1596-1599
   Abstract »    PDF »
Regulation of expression of mRNAs encoding the nerve growth factor receptors p75 and trkA in developing sensory neurons.
S Wyatt and A. Davies (1993)
Development 119, 635-648
   Abstract »    PDF »
The Extracellular Domain of p75NTR Is Necessary to Inhibit Neurotrophin-3 Signaling through TrkA.
P. S. Mischel, S. G. Smith, E. R. Vining, J. S. Valletta, W. C. Mobley, and L. F. Reichardt (2001)
J. Biol. Chem. 276, 11294-11301
   Abstract »    Full Text »    PDF »
The Cytoplasmic and Transmembrane Domains of the p75 and Trk A Receptors Regulate High Affinity Binding to Nerve Growth Factor.
D. Esposito, P. Patel, R. M. Stephens, P. Perez, M. V. Chao, D. R. Kaplan, and B. L. Hempstead (2001)
J. Biol. Chem. 276, 32687-32695
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)