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Science 26 April 1991:
Vol. 252. no. 5005, pp. 542 - 546
DOI: 10.1126/science.1708526
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Articles

Science, Vol 252, Issue 5005, 542-546
Copyright © 1991 by American Association for the Advancement of Science

articles

A class III transcription factor composed of RNA

LS Young, HM Dunstan, PR Witte, TP Smith, S Ottonello, and KU Sprague

Institute of Molecular Biology, University of Oregon, Eugene 97403.

It is generally assumed that the machinery that transcribes genes is composed entirely of polypeptides. However, in vitro transcription by silkworm RNA polymerase III requires a transcription factor that is not a polypeptide. This component, TFIIIR, is distinct from the previously identified transcription components: RNA polymerase III, and the accessory factors TFIIIA, TFIIIB, TFIIIC, and TFIIID. The newly discovered TFIIIR is a macromolecule that appears to be composed of RNA. It is resistant to heat, detergent, phenol, protease, and deoxyribonuclease, but it is sensitive to alkali and ribonuclease.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A heteromeric protein that binds to a meiotic homologous recombination hot spot: correlation of binding and hot spot activity..
W P Wahls and G R Smith (1994)
Genes & Dev. 8, 1693-1702
   Abstract »    PDF »
RNA associated with a heterodimeric protein that activates a meiotic homologous recombination hot spot: RL/RT/PCR strategy for cloning any unknown RNA or DNA..
W P Wahls (1994)
Genome Res. 3, 272-277
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)