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Science 19 April 1991:
Vol. 252. no. 5004, pp. 434 - 437
DOI: 10.1126/science.2017681
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Articles

Science, Vol 252, Issue 5004, 434-437
Copyright © 1991 by American Association for the Advancement of Science

articles

Group I intron self-splicing with adenosine: evidence for a single nucleoside-binding site

MD Been and AT Perrotta

Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.

For self-splicing of Tetrahymena ribosomal RNA precursor, guanosine binding is required for 5' splice-site cleavage and exon ligation. Whether these two reactions use the same or different guanosine-binding sites has been debated. A double mutation in a previously identified guanosine-binding site within the intron resulted in preference for adenosine (or adenosine triphosphate) as the substrate for cleavage at the 5' splice site. However, splicing was blocked in the exon ligation step. Blockage was reversed by a change from guanine to adenine at the 3' splice site. These results indicate that a single determinant specifies nucleoside binding for both steps of splicing. Furthermore, it suggests that RNA could form an active site specific for adenosine triphosphate.


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Activation of the catalytic core of a group I intron by a remote 3' splice junction..
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