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Science 29 March 1991:
Vol. 251. no. 5001, pp. 1597 - 1600
DOI: 10.1126/science.2011739
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Articles

Science, Vol 251, Issue 5001, 1597-1600
Copyright © 1991 by American Association for the Advancement of Science

articles

Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR

A Gatignol, A Buckler-White, B Berkhout, and KT Jeang

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.


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