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Science 22 March 1991:
Vol. 251. no. 5000, pp. 1490 - 1493
DOI: 10.1126/science.2006423
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Articles

Science, Vol 251, Issue 5000, 1490-1493
Copyright © 1991 by American Association for the Advancement of Science

articles

Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B

SM Ruben, PJ Dillon, R Schreck, T Henkel, CH Chen, M Maher, PA Baeuerle, and CA Rosen

Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110-1199.

A DNA probe that spanned a domain conserved among the proto-oncogene c-rel, the Drosophila morphogen dorsal, and the p50 DNA binding subunit of NF-kappa B was generated from Jurkat T cell complementary DNA with the polymerase chain reaction (PCR) and degenerate oligonucleotides. This probe was used to identify a rel-related complementary DNA that hybridized to a 2.6-kilobase messenger RNA present in human T and B lymphocytes. In vitro transcription and translation of the complementary DNA resulted in the synthesis of a protein with an apparent molecular size of 65 kilodaltons (kD). The translated protein showed weak DNA binding with a specificity for the kappa B binding motif. This protein-DNA complex comigrated with the complex obtained with the purified human p65 NF-kappa B subunit and binding was inhibited by I kappa B-alpha and -beta proteins. In addition, the 65-kD protein associated with the p50 subunit of NF-kappa B and the kappa B probe to form a complex with the same electrophoretic mobility as the NF-kappa B-DNA complex. Therefore the rel-related 65-kD protein may represent the p65 subunit of the active NF-kappa B transcription factor complex.


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Differential Transcriptional Activation in Vitro by NF-kappaB/Rel Proteins.
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   Abstract »    Full Text »    PDF »
NF-kappa B activation by ultraviolet light not dependent on a nuclear signal.
Y Devary, C Rosette, J. DiDonato, and M Karin (1993)
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   Abstract »    PDF »
The p65 subunit of NF-kappa B regulates I kappa B by two distinct mechanisms..
M L Scott, T Fujita, H C Liou, G P Nolan, and D Baltimore (1993)
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p105 and p98 precursor proteins play an active role in NF-kappa B-mediated signal transduction..
F Mercurio, J A DiDonato, C Rosette, and M Karin (1993)
Genes & Dev. 7, 705-718
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NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway.
S. Sun, P. Ganchi, D. Ballard, and W. Greene (1993)
Science 259, 1912-1915
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Ablation of transplanted HTLV-I tax-transformed tumors in mice by antisense inhibition of NF-kappa B.
I Kitajima, T Shinohara, J Bilakovics, D. Brown, X Xu, and M Nerenberg (1993)
Science 259, 1523
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Expression of relB transcripts during lymphoid organ development: specific expression in dendritic antigen-presenting cells.
D Carrasco, R. Ryseck, and R Bravo (1993)
Development 118, 1221-1231
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Homeostatic balance between dorsal and cactus proteins in the Drosophila embryo.
S. Govind, L. Brennan, and R. Steward (1993)
Development 117, 135-148
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I kappa B interacts with the nuclear localization sequences of the subunits of NF-kappa B: a mechanism for cytoplasmic retention..
A A Beg, S M Ruben, R I Scheinman, S Haskill, C A Rosen, and A S Baldwin (1992)
Genes & Dev. 6, 1899-1913
   Abstract »    PDF »
NF-kappa B subunit regulation in nontransformed CD4+ T lymphocytes.
S. Kang, A. Tran, M Grilli, and M. Lenardo (1992)
Science 256, 1452-1456
   Abstract »    PDF »
I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity..
S M Ruben, J F Klement, T A Coleman, M Maher, C H Chen, and C A Rosen (1992)
Genes & Dev. 6, 745-760
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Transcriptional regulation of the HIV-1 promoter by NF-kappa B in vitro..
M Kretzschmar, M Meisterernst, C Scheidereit, G Li, and R G Roeder (1992)
Genes & Dev. 6, 761-774
   Abstract »    PDF »
Independent modes of transcriptional activation by the p50 and p65 subunits of NF-kappa B..
T Fujita, G P Nolan, S Ghosh, and D Baltimore (1992)
Genes & Dev. 6, 775-787
   Abstract »    PDF »
Identification of a Naturally Occurring Transforming Variant of the p65 Subunit of NF-KB.
R. Narayanan, J. F. Klement, S. M. Ruben, K. A. Higgins, and C. A. Rosen (1992)
Science 256, 367-370
   Abstract »    PDF »
The functional domains of the Drosophila morphogen dorsal: evidence from the analysis of mutants..
K Isoda, S Roth, and C Nusslein-Volhard (1992)
Genes & Dev. 6, 619-630
   Abstract »    PDF »
Rel-associated pp40: an inhibitor of the rel family of transcription factors.
N Davis, S Ghosh, D. Simmons, P Tempst, H. Liou, D Baltimore, and H. Bose Jr (1991)
Science 253, 1268-1271
   Abstract »    PDF »
The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation..
L D Kerr, J Inoue, N Davis, E Link, P A Baeuerle, H R Bose, and I M Verma (1991)
Genes & Dev. 5, 1464-1476
   Abstract »    PDF »
Dynamics of NF kappa B and Ikappa Balpha Studied with Green Fluorescent Protein (GFP) Fusion Proteins. INVESTIGATION OF GFP-p65 BINDING TO DNA BY FLUORESCENCE RESONANCE ENERGY TRANSFER.
J. A. Schmid, A. Birbach, R. Hofer-Warbinek, M. Pengg, U. Burner, P. G. Furtmuller, B. R. Binder, and R. de Martin (2000)
J. Biol. Chem. 275, 17035-17042
   Abstract »    Full Text »    PDF »
Ikappa Balpha and Ikappa Balpha /NF-kappa B Complexes Are Retained in the Cytoplasm through Interaction with a Novel Partner, RasGAP SH3-binding Protein 2.
M. Prigent, I. Barlat, H. Langen, and C. Dargemont (2000)
J. Biol. Chem. 275, 36441-36449
   Abstract »    Full Text »    PDF »
Regulation of an Endogenous Locus Using a Panel of Designed Zinc Finger Proteins Targeted to Accessible Chromatin Regions. ACTIVATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR A.
P.-Q. Liu, E. J. Rebar, L. Zhang, Q. Liu, A. C. Jamieson, Y. Liang, H. Qi, P.-X. Li, B. Chen, M. C. Mendel, et al. (2001)
J. Biol. Chem. 276, 11323-11334
   Abstract »    Full Text »    PDF »


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