Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 February 1991:
Vol. 251. no. 4996, pp. 939 - 942
DOI: 10.1126/science.2000494
Prev | Table of Contents | Next

Articles

Science, Vol 251, Issue 4996, 939-942
Copyright © 1991 by American Association for the Advancement of Science

articles

Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel

G Yellen, ME Jurman, T Abramson, and R MacKinnon

Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

The active site of voltage-activated potassium channels is a transmembrane aqueous pore that permits ions to permeate the cell membrane in a rapid yet highly selective manner. A useful probe for the pore of potassium-selective channels is the organic ion tetraethylammonium (TEA), which binds with millimolar affinity to the intracellular opening of the pore and blocks potassium current. In the potassium channel encoded by the Drosophila Shaker gene, an amino acid residue that specifically affects the affinity for intracellular TEA has now been identified by site-directed mutagenesis. This residue is in the middle of a conserved stretch of 18 amino acids that separates two locations that are both near the external opening of the pore. These findings suggest that this conserved region is intimately involved in the formation of the ion conduction pore of voltage-activated potassium channels. Further, a stretch of only eight amino acid residues must traverse 80 percent of the transmembrane electric potential difference.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mutational and In Silico Analyses for Antidepressant Block of Astroglial Inward-Rectifier Kir4.1 Channel.
K. Furutani, Y. Ohno, A. Inanobe, H. Hibino, and Y. Kurachi (2009)
Mol. Pharmacol. 75, 1287-1295
   Abstract »    Full Text »    PDF »
Blocking Pore-open Mutants of CLC-0 by Amphiphilic Blockers.
X.-D. Zhang, P.-Y. Tseng, W.-P. Yu, and T.-Y. Chen (2008)
J. Gen. Physiol. 133, 43-58
   Abstract »    Full Text »    PDF »
Intracellular Mg2+ is a voltage-dependent pore blocker of HCN channels.
S. Vemana, S. Pandey, and H. P. Larsson (2008)
Am J Physiol Cell Physiol 295, C557-C565
   Abstract »    Full Text »    PDF »
Molecular Characterization of the Inositol 1,4,5-Trisphosphate Receptor Pore-forming Segment.
Z. T. Schug, P. C. A. da Fonseca, C. D. Bhanumathy, L. Wagner II, X. Zhang, B. Bailey, E. P. Morris, D. I. Yule, and S. K. Joseph (2008)
J. Biol. Chem. 283, 2939-2948
   Abstract »    Full Text »    PDF »
Probing the Cavity of the Slow Inactivated Conformation of Shaker Potassium Channels.
G. Panyi and C. Deutsch (2007)
J. Gen. Physiol. 129, 403-418
   Abstract »    Full Text »    PDF »
State-independent Block of BK Channels by an Intracellular Quaternary Ammonium.
C. M. Wilkens and R. W. Aldrich (2006)
J. Gen. Physiol. 128, 347-364
   Abstract »    Full Text »    PDF »
Overview of Molecular Relationships in the Voltage-Gated Ion Channel Superfamily.
F. H. Yu, V. Yarov-Yarovoy, G. A. Gutman, and W. A. Catterall (2005)
Pharmacol. Rev. 57, 387-395
   Full Text »    PDF »
Status of the Intracellular Gate in the Activated-not-open State of Shaker K+ Channels.
D. del Camino, M. Kanevsky, and G. Yellen (2005)
J. Gen. Physiol. 126, 419-428
   Abstract »    Full Text »    PDF »
Separation of P/C- and U-type inactivation pathways in Kv1.5 potassium channels.
H. T Kurata, K. W Doerksen, J. R Eldstrom, S. Rezazadeh, and D. Fedida (2005)
J. Physiol. 568, 31-46
   Abstract »    Full Text »    PDF »
Two Stable, Conducting Conformations of the Selectivity Filter in Shaker K+ Channels.
J. Thompson and T. Begenisich (2005)
J. Gen. Physiol. 125, 619-629
   Abstract »    Full Text »    PDF »
Kv1 K+ Channels Control Purkinje Cell Output to Facilitate Postsynaptic Rebound Discharge in Deep Cerebellar Neurons.
B. E. McKay, M. L. Molineux, W. H. Mehaffey, and R. W. Turner (2005)
J. Neurosci. 25, 1481-1492
   Abstract »    Full Text »    PDF »
Three Mechanisms Underlie KCNQ2/3 Heteromeric Potassium M-Channel Potentiation.
A. Etxeberria, I. Santana-Castro, M. P. Regalado, P. Aivar, and A. Villarroel (2004)
J. Neurosci. 24, 9146-9152
   Abstract »    Full Text »    PDF »
The VGL-Chanome: A Protein Superfamily Specialized for Electrical Signaling and Ionic Homeostasis.
F. H. Yu and W. A. Catterall (2004)
Sci. STKE 2004, re15
   Abstract »    Full Text »    PDF »
Mechanism of Block of hEag1 K+ Channels by Imipramine and Astemizole.
R. E. Garcia-Ferreiro, D. Kerschensteiner, F. Major, F. Monje, W. Stuhmer, and L. A. Pardo (2004)
J. Gen. Physiol. 124, 301-317
   Abstract »    Full Text »    PDF »
Reversible Pore Block of Connexin Channels by Cyclodextrins.
D. Locke, I. V. Koreen, J. Y. Liu, and A. L. Harris (2004)
J. Biol. Chem. 279, 22883-22892
   Abstract »    Full Text »    PDF »
Outer Pore Architecture of a Ca2+-selective TRP Channel.
T. Voets, A. Janssens, G. Droogmans, and B. Nilius (2004)
J. Biol. Chem. 279, 15223-15230
   Abstract »    Full Text »    PDF »
An Apamin- and Scyllatoxin-Insensitive Isoform of the Human SK3 Channel.
O. H. Wittekindt, V. Visan, H. Tomita, F. Imtiaz, J. J. Gargus, F. Lehmann-Horn, S. Grissmer, and D. J. Morris-Rosendahl (2004)
Mol. Pharmacol. 65, 788-801
   Abstract »    Full Text »    PDF »
Molecular Basis for Kv1.5 Channel Block: CONSERVATION OF DRUG BINDING SITES AMONG VOLTAGE-GATED K+ CHANNELS.
N. Decher, B. Pirard, F. Bundis, S. Peukert, K.-H. Baringhaus, A. E. Busch, K. Steinmeyer, and M. C. Sanguinetti (2004)
J. Biol. Chem. 279, 394-400
   Abstract »    Full Text »    PDF »
Pseudechetoxin Binds to the Pore Turret of Cyclic Nucleotide-gated Ion Channels.
R. L. Brown, L. L. Lynch, T. L. Haley, and R. Arsanjani (2003)
J. Gen. Physiol. 122, 749-760
   Abstract »    Full Text »    PDF »
External TEA Block of Shaker K+ Channels Is Coupled to the Movement of K+ Ions within the Selectivity Filter.
J. Thompson and T. Begenisich (2003)
J. Gen. Physiol. 122, 239-246
   Abstract »    Full Text »    PDF »
Voltage-Gated K Channels.
C. M. Armstrong (2003)
Sci. STKE 2003, re10
   Abstract »    Full Text »    PDF »
C-Type inactivation involves a significant decrease in the intracellular aqueous pore volume of Kv1.4 K+ channels expressed in Xenopus oocytes.
X. Jiang, G. C L Bett, X. Li, V. E Bondarenko, and R. L Rasmusson (2003)
J. Physiol. 549, 683-695
   Abstract »    Full Text »    PDF »
Local Anesthetic Block of Kv Channels: Role of the S6 Helix and the S5-S6 Linker for Bupivacaine Action.
J. Nilsson, M. Madeja, and P. Arhem (2003)
Mol. Pharmacol. 63, 1417-1429
   Abstract »    Full Text »    PDF »
Functional identification of ion binding sites at the internal end of the pore in Shaker K+ channels.
J. Thompson and T. Begenisich (2003)
J. Physiol. 549, 107-120
   Abstract »    Full Text »    PDF »
Molecular Site of Action of the Antiarrhythmic Drug Propafenone at the Voltage-Operated Potassium Channel Kv2.1.
M. Madeja, T. Leicher, P. Friederich, M. A. Punke, W. Haverkamp, U. Musshoff, G. Breithardt, and E.-J. Speckmann (2003)
Mol. Pharmacol. 63, 547-556
   Abstract »    Full Text »    PDF »
Probing an Open CFTR Pore with Organic Anion Blockers.
Z. Zhou, S. Hu, and T.-C. Hwang (2002)
J. Gen. Physiol. 120, 647-662
   Abstract »    Full Text »    PDF »
Putative binding sites for benzocaine on a human cardiac cloned channel (Kv1.5).
R. Caballero, I. Moreno, T. Gonzalez, C. Valenzuela, J. Tamargo, and E. Delpon (2002)
Cardiovasc Res 56, 104-117
   Abstract »    Full Text »    PDF »
Site-directed Glycosylation Tagging of Functional Kir2.1 Reveals That the Putative Pore-forming Segment Is Extracellular.
R. A. Schwalbe, A. Rudin, S.-L. Xia, and C. S. Wingo (2002)
J. Biol. Chem. 277, 24382-24389
   Abstract »    Full Text »    PDF »
The Binding Site for Channel Blockers That Rescue Misprocessed Human Long QT Syndrome Type 2 ether-a-gogo-related Gene (HERG) Mutations.
E. Ficker, C. A. Obejero-Paz, S. Zhao, and A. M. Brown (2002)
J. Biol. Chem. 277, 4989-4998
   Abstract »    Full Text »    PDF »
Gating and Conductance Properties of Bk Channels Are Modulated by the S9-S10 Tail Domain of the {alpha} Subunit: A Study of Mslo1 and Mslo3 Wild-Type and Chimeric Channels.
B. L. Moss and K. L. Magleby (2001)
J. Gen. Physiol. 118, 711-734
   Abstract »    Full Text »    PDF »
Functional Analysis of Capsaicin Receptor (Vanilloid Receptor Subtype 1) Multimerization and Agonist Responsiveness Using a Dominant Negative Mutation.
E. V. Kuzhikandathil, H. Wang, T. Szabo, N. Morozova, P. M. Blumberg, and G. S. Oxford (2001)
J. Neurosci. 21, 8697-8706
   Abstract »    Full Text »    PDF »
Hidden Markov Model Analysis of Intermediate Gating Steps Associated with the Pore Gate of Shaker Potassium Channels.
J. Zheng, L. Vankataramanan, and F. J. Sigworth (2001)
J. Gen. Physiol. 118, 547-564
   Abstract »    Full Text »    PDF »
Extracellular Blockade of K+ Channels by Tea: Results from Molecular Dynamics Simulations of the Kcsa Channel.
S. Crouzy, S. Berneche, and B. Roux (2001)
J. Gen. Physiol. 118, 207-218
   Abstract »    Full Text »    PDF »
Molecular Architecture of the Voltage-Dependent Na Channel: Functional Evidence for {alpha} Helices in the Pore.
T. Yamagishi, R. A. Li, K. Hsu, E. Marban, and G. F. Tomaselli (2001)
J. Gen. Physiol. 118, 171-182
   Abstract »    Full Text »    PDF »
Selective Open-Channel Block of Shaker (Kv1) Potassium Channels by S-Nitrosodithiothreitol (Sndtt).
M. W. Brock, C. Mathes, and W. F. Gilly (2001)
J. Gen. Physiol. 118, 113-134
   Abstract »    Full Text »    PDF »
Affinity and Location of an Internal K+ Ion Binding Site in Shaker K Channels.
J. Thompson and T. Begenisich (2001)
J. Gen. Physiol. 117, 373-384
   Abstract »    Full Text »    PDF »
Oxidative Regulation of Large Conductance Calcium-Activated Potassium Channels.
X. D. Tang, H. Daggett, M. Hanner, M. L. Garcia, O. B. McManus, N. Brot, H. Weissbach, S. H. Heinemann, and T. Hoshi (2001)
J. Gen. Physiol. 117, 253-274
   Abstract »    Full Text »    PDF »
Cardiac sodium channel and inherited arrhythmia syndromes.
C. R Bezzina, M. B Rook, and A. A.M Wilde (2001)
Cardiovasc Res 49, 257-271
   Full Text »    PDF »
Potassium Transport in the Mammalian Collecting Duct.
S. Muto (2001)
Physiol Rev 81, 85-116
   Abstract »    Full Text »    PDF »
Tea+-Sensitive Kcnq1 Constructs Reveal Pore-Independent Access to Kcne1 in Assembled IKs Channels.
J. Kurokawa, H.K. Motoike, and R.S. Kass (2001)
J. Gen. Physiol. 117, 43-52
   Abstract »    Full Text »    PDF »
GYGD pore motifs in neighbouring potassium channel subunits interact to determine ion selectivity.
M. L Chapman, H. S Krovetz, and A. M J VanDongen (2001)
J. Physiol. 530, 21-33
   Abstract »    Full Text »    PDF »
Potassium Channels: Molecular Defects, Diseases, and Therapeutic Opportunities.
C.-C. Shieh, M. Coghlan, J. P. Sullivan, and M. Gopalakrishnan (2000)
Pharmacol. Rev. 52, 557-594
   Abstract »    Full Text »    PDF »
Interaction between Quaternary Ammonium Ions in the Pore of Potassium Channels: Evidence against an Electrostatic Repulsion Mechanism.
J. Thompson and T. Begenisich (2000)
J. Gen. Physiol. 115, 769-782
   Abstract »    Full Text »    PDF »
Tertiapin Potently and Selectively Blocks Muscarinic K+ Channels in Rabbit Cardiac Myocytes.
H. Kitamura, M. Yokoyama, H. Akita, K. Matsushita, Y. Kurachi, and M. Yamada (2000)
J. Pharmacol. Exp. Ther. 293, 196-205
   Abstract »    Full Text »
Permeation of Large Tetra-Alkylammonium Cations through Mutant and Wild-Type Voltage-Gated Sodium Channels as Revealed by Relief of Block at High Voltage.
C.-J. Huang, I. Favre, and E. Moczydlowski (2000)
J. Gen. Physiol. 115, 435-454
   Abstract »    Full Text »    PDF »
The Accessibility of a Novel Reentrant Loop of the Glutamate Transporter GLT-1 Is Restricted by Its Substrate.
M. Grunewald and B. I. Kanner (2000)
J. Biol. Chem. 275, 9684-9689
   Abstract »    Full Text »    PDF »
Characterization of the Selectivity Filter of the Epithelial Sodium Channel.
S. Sheng, J. Li, K. A. McNulty, D. Avery, and T. R. Kleyman (2000)
J. Biol. Chem. 275, 8572-8581
   Abstract »    Full Text »    PDF »
Charged residues in the M2 region of alpha -hENaC play a role in channel conductance.
A. L. B. Langloh, B. Berdiev, H.-L. Ji, K. Keyser, B. A. Stanton, and D. J. Benos (2000)
Am J Physiol Cell Physiol 278, C277-C291
   Abstract »    Full Text »    PDF »
Ultrafast Inactivation Causes Inward Rectification in a Voltage-Gated K+ Channel from Caenorhabditis elegans.
R. Fleischhauer, M. W. Davis, I. Dzhura, A. Neely, L. Avery, and R. H. Joho (2000)
J. Neurosci. 20, 511-520
   Abstract »    Full Text »    PDF »
Permeation through the CFTR chloride channel.
N. McCarty (2000)
J. Exp. Biol. 203, 1947-1962
   Abstract »    PDF »
{alpha}-Helical Structural Elements within the Voltage-Sensing Domains of a K+ Channel.
Y. Li-Smerin, D. H. Hackos, and K. J. Swartz (2000)
J. Gen. Physiol. 115, 33-50
   Abstract »    Full Text »    PDF »
A Pore Segment in DEG/ENaC Na+ Channels.
P. M. Snyder, D. R. Olson, and D. B. Bucher (1999)
J. Biol. Chem. 274, 28484-28490
   Abstract »    Full Text »    PDF »
Single Streptomyces lividans K+ Channels: Functional Asymmetries and Sidedness of Proton Activation.
L. Heginbotham, M. LeMasurier, L. Kolmakova-Partensky, and C. Miller (1999)
J. Gen. Physiol. 114, 551-560
   Abstract »    Full Text »    PDF »
Voltage-Gated Ion Channels and Hereditary Disease.
F. Lehmann-Horn and K. Jurkat-Rott (1999)
Physiol Rev 79, 1317-1372
   Abstract »    Full Text »    PDF »
Tuning the Voltage Dependence of Tetraethylammonium Block with Permeant Ions in an Inward-Rectifier K+ Channel.
M. Spassova and Z. Lu (1999)
J. Gen. Physiol. 114, 415-426
   Abstract »    Full Text »    PDF »
Local Anesthetic Anchoring to Cardiac Sodium Channels : Implications Into Tissue-Selective Drug Targeting.
R. A. Li, R. G. Tsushima, K. Himmeldirk, D. S. Dime, and P. H. Backx (1999)
Circ. Res. 85, 88-98
   Abstract »    Full Text »    PDF »
Structure and function of the cardiac sodium channels.
J. R. Balser (1999)
Cardiovasc Res 42, 327-328
   Abstract »    Full Text »    PDF »
Identification by Differential Display of a Hypertonicity-inducible Inward Rectifier Potassium Channel Highly Expressed in Chloride Cells.
Y. Suzuki, M. Itakura, M. Kashiwagi, N. Nakamura, T. Matsuki, H. Sakuta, N. Naito, K. Takano, T. Fujita, and S. Hirose (1999)
J. Biol. Chem. 274, 11376-11382
   Abstract »    Full Text »    PDF »
Helical Structure and Packing Orientation of the S2 Segment in the Shaker K+ Channel.
S. A. Monks, D. J. Needleman, and C. Miller (1999)
J. Gen. Physiol. 113, 415-423
   Abstract »    Full Text »    PDF »
The Epithelial Inward Rectifier Channel Kir7.1 Displays Unusual K+ Permeation Properties.
F. Doring, C. Derst, E. Wischmeyer, C. Karschin, R. Schneggenburger, J. Daut, and A. Karschin (1998)
J. Neurosci. 18, 8625-8636
   Abstract »    Full Text »    PDF »
Subunit Folding and Assembly Steps Are Interspersed during Shaker Potassium Channel Biogenesis.
C. T. Schulteis, N. Nagaya, and D. M. Papazian (1998)
J. Biol. Chem. 273, 26210-26217
   Abstract »    Full Text »    PDF »
Antagonism by Class I Antiarrhythmic Drugs of Levcromakalim-Induced Relaxation in Isolated Rat Aorta.
A. L. Cogolludo, F. Perez-Vizcaino, and J. Tamargo (1998)
J. Pharmacol. Exp. Ther. 287, 81-86
   Abstract »    Full Text »
Transfer of rapid inactivation and sensitivity to the class III antiarrhythmic drug E-4031 from HERG to M-eag channels.
I. M Herzberg, M. C Trudeau, and G. A Robertson (1998)
J. Physiol. 511, 3-14
   Abstract »    Full Text »    PDF »
The selectivity filter of a potassium channel, murine Kir2.1, investigated using scanning cysteine mutagenesis.
C Dart, M L Leyland, P J Spencer, P R Stanfield, and M J Sutcliffe (1998)
J. Physiol. 511, 25-32
   Abstract »    Full Text »    PDF »
Coupled Ion Movement Underlies Rectification in an Inward-Rectifier K+ Channel.
M. Spassova and Z. Lu (1998)
J. Gen. Physiol. 112, 211-221
   Abstract »    Full Text »    PDF »
Structural Determinants of Potency and Stereoselective Block of hKv1.5 Channels Induced by Local Anesthetics.
M. Longobardo, E. Delpón, R. Caballero, J. Tamargo, and C. Valenzuela (1998)
Mol. Pharmacol. 54, 162-169
   Abstract »    Full Text »
Structure and function of voltage-gated sodium channels.
E. Marban, T. Yamagishi, and G. F Tomaselli (1998)
J. Physiol. 508, 647-657
   Abstract »    Full Text »    PDF »
The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity.
D. A. Doyle, J. M. Cabral, R. A. Pfuetzner, A. Kuo, J. M. Gulbis, S. L. Cohen, B. T. Chait, and R. MacKinnon (1998)
Science 280, 69-77
   Abstract »    Full Text »    PDF »
Molecular Determinants of Stereoselective Bupivacaine Block of hKv1.5 Channels.
L. Franqueza, M. Longobardo, J. Vicente, E. Delpon, M. M. Tamkun, J. Tamargo, D. J. Snyders, and C. Valenzuela (1997)
Circ. Res. 81, 1053-1064
   Abstract »    Full Text »
Alternative Splicing in the Pore-Forming Region of shaker Potassium Channels.
M. Kim, D. J. Baro, C. C. Lanning, M. Doshi, J. Farnham, H. S. Moskowitz, J. H. Peck, B. M. Olivera, and R. M. Harris-Warrick (1997)
J. Neurosci. 17, 8213-8224
   Abstract »    Full Text »    PDF »
Mapping the Kidney Potassium Channel ROMK1. GLYCOSYLATION OF THE PORE SIGNATURE SEQUENCE AND THE COOH TERMINUS.
R. A. Schwalbe, L. Bianchi, and A. M. Brown (1997)
J. Biol. Chem. 272, 25217-25223
   Abstract »    Full Text »    PDF »
Selectivity Changes during Activation of Mutant Shaker Potassium Channels.
J. Zheng and F. J. Sigworth (1997)
J. Gen. Physiol. 110, 101-117
   Abstract »    Full Text »    PDF »
Control of Rectification and Gating of Cloned KATP Channels by the Kir6.2 Subunit.
S.-L. Shyng, T. Ferrigni, and C.G. Nichols (1997)
J. Gen. Physiol. 110, 141-153
   Abstract »    Full Text »    PDF »
P-loop Flexibility in Na+ Channel Pores Revealed by Single- and Double-cysteine Replacements.
R. G. Tsushima, R. A. Li, and P. H. Backx (1997)
J. Gen. Physiol. 110, 59-72
   Abstract »    Full Text »    PDF »
Role of Transmembrane Segment S5 on Gating of Voltage-dependent K+ Channels.
C.-C. Shieh, K. G. Klemic, and G. E. Kirsch (1997)
J. Gen. Physiol. 109, 767-778
   Abstract »    Full Text »    PDF »
Scanning mutagenesis of the putative transmembrane segments of Kir2.1, an inward rectifier potassium channel.
A. Collins, H.-h. Chuang, Y. N. Jan, and L. Y. Jan (1997)
PNAS 94, 5456-5460
   Abstract »    Full Text »    PDF »
Intrinsic Voltage Dependence and Ca2+ Regulation of mslo Large Conductance Ca-activated K+ Channels.
J. Cui, D.H. Cox, and R.W. Aldrich (1997)
J. Gen. Physiol. 109, 647-673
   Abstract »    Full Text »    PDF »
A Closer Picture of the K Channel Gate from Ion Trapping Experiments.
C. M. Armstrong (1997)
J. Gen. Physiol. 109, 523-524
   Full Text »    PDF »
Altered Ionic Selectivity of the Sodium Channel Revealed by Cysteine Mutations within the Pore.
R. G. Tsushima, R. A. Li, and P. H. Backx (1997)
J. Gen. Physiol. 109, 463-475
   Abstract »    Full Text »    PDF »
Two functionally distinct subsites for the binding of internal blockers to the pore of voltage-activated K+ channels.
T. Baukrowitz and G. Yellen (1996)
PNAS 93, 13357-13361
   Abstract »    Full Text »    PDF »
Novel Sites of N-Glycosylation in ROMK1 Reveal the Putative Pore-forming Segment H5 as Extracellular.
R. A. Schwalbe, Z. Wang, L. Bianchi, and A. M. Brown (1996)
J. Biol. Chem. 271, 24201-24206
   Abstract »    Full Text »    PDF »
Class III Antiarrhythmic Effects of Zatebradine: Time-, State-, Use-, and Voltage-Dependent Block of hKv1.5 Channels.
C. Valenzuela, E. Delpon, L. Franqueza, P. Gay, O. Perez, J. Tamargo, and D. J. Snyders (1996)
Circulation 94, 562-570
   Abstract »    Full Text »
Molecular Analysis of a Binding Site for Quinidine in a Human Cardiac Delayed Rectifier K+ Channel : Role of S6 in Antiarrhythmic Drug Binding.
S. W. Yeola, T. C. Rich, V. N. Uebele, M. M. Tamkun, and D. J. Snyders (1996)
Circ. Res. 78, 1105-1114
   Abstract »    Full Text »
Inward Rectification and Implications for Cardiac Excitability.
C.G. Nichols, E.N. Makhina, W.L. Pearson, Q. Sha, and A.N. Lopatin (1996)
Circ. Res. 78, 1-7
   Abstract »    Full Text »
Determinants of Antiarrhythmic Drug Action : Electrostatic and Hydrophobic Components of Block of the Human Cardiac hKv1.5 Channel.
D. J. Snyders and S. W. Yeola (1995)
Circ. Res. 77, 575-583
   Abstract »    Full Text »
Alcohols Inhibit a Cloned Potassium Channel at a Discrete Saturable Site.
M. Covarrubias, T. B. Vyas, L. Escobar, and A. Wei (1995)
J. Biol. Chem. 270, 19408-19416
   Abstract »    Full Text »    PDF »
Silver as a probe of pore-forming residues in a potassium channel.
Q Lu and C Miller (1995)
Science 268, 304-307
   Abstract »    PDF »
Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor.
P Hidalgo and R MacKinnon (1995)
Science 268, 307-310
   Abstract »    PDF »
A Topological Analysis of Goldfish Kainate Receptors Predicts Three Transmembrane Segments.
Z. G. Wo and R. E. Oswald (1995)
J. Biol. Chem. 270, 2000-2009
   Abstract »    Full Text »    PDF »
Folding pattern diversity of integral membrane proteins.
S. Cowan and J. Rosenbusch (1994)
Science 264, 914-916
   PDF »
Specification of pore properties by the carboxyl terminus of inwardly rectifying K+ channels.
M Taglialatela, B. Wible, R Caporaso, and A. Brown (1994)
Science 264, 844-847
   Abstract »    PDF »
Functional stoichiometry of Shaker potassium channel inactivation.
R MacKinnon, R. Aldrich, and A. Lee (1993)
Science 262, 757-759
   Abstract »    PDF »
A mechanism for ion selectivity in potassium channels: computational studies of cation-pi interactions.
R. Kumpf and D. Dougherty (1993)
Science 261, 1708-1710
   Abstract »    PDF »
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
D. Schachtman, J. Schroeder, W. Lucas, J. Anderson, and R. Gaber (1992)
Science 258, 1654-1658
   Abstract »    PDF »
A functional connection between the pores of distantly related ion channels as revealed by mutant K+ channels.
L Heginbotham, T Abramson, and R MacKinnon (1992)
Science 258, 1152-1155
   Abstract »    PDF »
Acetylcholine receptor channel structure probed in cysteine-substitution mutants.
M. Akabas, D. Stauffer, M Xu, and A Karlin (1992)
Science 258, 307-310
   Abstract »    PDF »
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
M Li, Y. Jan, and L. Jan (1992)
Science 257, 1225-1230
   Abstract »    PDF »
Molecular localization of an ion-binding site within the pore of mammalian sodium channels.
P. Backx, D. Yue, J. Lawrence, E Marban, and G. Tomaselli (1992)
Science 257, 248-251
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)