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Science 15 February 1991:
Vol. 251. no. 4995, pp. 807 - 810
DOI: 10.1126/science.1990443
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Articles

Science, Vol 251, Issue 4995, 807-810
Copyright © 1991 by American Association for the Advancement of Science

articles

Translational potentiation of messenger RNA with secondary structure in Xenopus

LN Fu, RQ Ye, LW Browder, and RN Johnston

Department of Biological Sciences, University of Calgary, Alberta, Canada.

Differential translation of messenger RNA (mRNA) with stable secondary structure in the 5' untranslated leader may contribute to the dramatic changes in protein synthetic patterns that occur during oogenesis and early development. Plasmids that contained the bacterial gene chloramphenicol acetyltransferase and which encoded mRNA with (hpCAT) or without (CAT) a stable hairpin secondary structure in the 5' noncoding region were transcribed in vitro, and the resulting mRNAs were injected into Xenopus oocytes, eggs, and early embryos. During early oogenesis, hpCAT mRNA was translated at less than 3 percent of the efficiency of CAT mRNA. The relative translational potential of hpCAT reached 100 percent in the newly fertilized egg and returned to approximately 3 percent after the midblastula transition.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Acute Box cis-Element in Human Heavy Ferritin mRNA 5'-Untranslated Region Is a Unique Translation Enhancer That Binds Poly(C)-binding Proteins.
A. M. Thomson, C. M. Cahill, H.-H. Cho, K. D. Kassachau, M. R. Epis, K. R. Bridges, P. J. Leedman, and J. T. Rogers (2005)
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Translational Regulation of Human Neuronal Nitric-oxide Synthase by an Alternatively Spliced 5'-Untranslated Region Leader Exon.
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J. Biol. Chem. 278, 636-644
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Kinetic Analysis of Phospholipase C[IMAGE] Isoforms Using Phospholipid-Detergent Mixed Micelles.
S. R. James, A. Paterson, T. K. Harden, and C. P. Downes (1995)
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The Translational Repression Mediated by the Platelet-derived Growth Factor 2/c- sis mRNA Leader Is Relieved during Megakaryocytic Differentiation.
J. Bernstein, I. Shefler, and O. Elroy-Stein (1995)
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Induction of mesoderm in Xenopus laevis embryos by translation initiation factor 4E.
P. Klein and D. Melton (1994)
Science 265, 803-806
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