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Science 8 February 1991:
Vol. 251. no. 4994, pp. 668 - 671
DOI: 10.1126/science.1704150
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Articles

Science, Vol 251, Issue 4994, 668-671
Copyright © 1991 by American Association for the Advancement of Science

articles

Inability of malaria vaccine to induce antibodies to a protective epitope within its sequence

Y Charoenvit, WE Collins, TR Jones, P Millet, L Yuan, GH Campbell, RL Beaudoin, Broderson JR, and SL Hoffman

Infectious Diseases Department, Naval Medical Research Institute, Bethesda, MD 20889.

Saimiri monkeys immunized with a recombinant protein containing 20 copies of the nine amino acid repeat of the Plasmodium vivax circumsporozoite (CS) protein developed high concentrations of antibodies to the repeat sequence and to sporozoites, but were not protected against challenge. After intravenous injection of an immunoglobulin G3 monoclonal antibody (NVS3) against irradiated P. vivax sporozoites, four of six monkeys were protected against sporozoite-induced malaria, and the remaining two animals took significantly longer to become parasitemic. Epitope mapping demonstrated that NVS3 recognizes only four (AGDR) of the nine amino acids within the repeat region of the P. vivax CS protein. The monkeys immunized with (DRAADGQPAG)20 did not produce antibodies to the protective epitope AGDR. Thus, determination of the fine specificity of protective immune responses may be critical to the construction of successful subunit vaccines.


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