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Science 1 February 1991:
Vol. 251. no. 4993, pp. 568 - 570
DOI: 10.1126/science.1703661
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Articles

Science, Vol 251, Issue 4993, 568-570
Copyright © 1991 by American Association for the Advancement of Science

articles

ACh receptor-rich membrane domains organized in fibroblasts by recombinant 43-kildalton protein

WD Phillips, C Kopta, P Blount, PD Gardner, JH Steinbach, and JP Merlie

Department of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.

Neurotransmitter receptors are generally clustered in the postsynaptic membrane. The mechanism of clustering was analyzed with fibroblast cell lines that were stably transfected with the four subunits for fetal (alpha, beta, gamma, delta) or adult (alpha, beta, epsilon, delta) type mouse muscle nicotinic acetylcholine receptors (AChRs). Immunofluorescent staining indicated that AChRs were dispersed on the surface of these cells. When transiently transfected with an expression construct encoding a 43-kilodalton protein that is normally concentrated under the postsynaptic membrane, AChRs expressed in these cells became aggregated in large cell-surface clusters, colocalized with the 43-kilodalton protein. This suggests that 43-kilodalton protein can induce AChR clustering and that cluster induction involves direct contact between AChR and 43-kilodalton protein.


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