Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 January 1991:
Vol. 251. no. 4992, pp. 426 - 430
DOI: 10.1126/science.1671176
Prev | Table of Contents | Next

Articles

Science, Vol 251, Issue 4992, 426-430
Copyright © 1991 by American Association for the Advancement of Science

articles

A genetic model for interaction of the homeodomain recognition helix with DNA

SD Hanes and R Brent

Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.

The Bicoid homeodomain protein controls anterior development in the Drosophila embryo by binding to DNA and regulating gene expression. With the use of genetic assays in yeast, the interaction between the Bicoid homeodomain and a series of mutated DNA sites was studied. These experiments defined important features of homeodomain binding sites, identified specific amino acid-base pair contacts, and suggested a model for interaction of the recognition alpha-helices of Bicoid and Antennapedia-class homeodomain proteins with DNA. The model is in general agreement with results of crystallographic and magnetic resonance studies, but differs in important details. It is likely that genetic studies of protein-DNA interaction will continue to complement conventional structural approaches.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In vitro analysis of DNA-protein interactions by proximity ligation.
S. M. Gustafsdottir, J. Schlingemann, A. Rada-Iglesias, E. Schallmeiner, M. Kamali-Moghaddam, C. Wadelius, and U. Landegren (2007)
PNAS 104, 3067-3072
   Abstract »    Full Text »    PDF »
Studying statistical properties of regulatory DNA sequences, and their use in predicting regulatory regions in the eukaryotic genomes.
I. Abnizova and W. R. Gilks (2006)
Brief Bioinform 7, 48-54
   Abstract »    Full Text »    PDF »
CHX10 Targets a Subset of Photoreceptor Genes.
K. M. Dorval, B. P. Bobechko, H. Fujieda, S. Chen, D. J. Zack, and R. Bremner (2006)
J. Biol. Chem. 281, 744-751
   Abstract »    Full Text »    PDF »
Exo-Taq-Based Detection of DNA-Binding Protein for Homogeneous and Microarray Format.
T. Fukumori, H. Miyachi, and K. Yokoyama (2005)
J. Biochem. 138, 473-478
   Abstract »    Full Text »    PDF »
Bicoid cooperative DNA binding is critical for embryonic patterning in Drosophila.
D. Lebrecht, M. Foehr, E. Smith, F. J. P. Lopes, C. E. Vanario-Alonso, J. Reinitz, D. S. Burz, and S. D. Hanes (2005)
PNAS 102, 13176-13181
   Abstract »    Full Text »    PDF »
Transcriptional Activity of the Paired-like Homeodomain Proteins CHX10 and VSX1.
K. M. Dorval, B. P. Bobechko, K. F. Ahmad, and R. Bremner (2005)
J. Biol. Chem. 280, 10100-10108
   Abstract »    Full Text »    PDF »
A phage display selection of engrailed homeodomain mutants and the importance of residue Q50.
M. D. Simon, K. Sato, G. A. Weiss, and K. M. Shokat (2004)
Nucleic Acids Res. 32, 3623-3631
   Abstract »    Full Text »    PDF »
Otx2 regulates the extent, identity and fate of neuronal progenitor domains in the ventral midbrain.
E. Puelles, A. Annino, F. Tuorto, A. Usiello, D. Acampora, T. Czerny, C. Brodski, S.-L. Ang, W. Wurst, and A. Simeone (2004)
Development 131, 2037-2048
   Abstract »    Full Text »    PDF »
A Short, Highly Active Photoreceptor-Specific Enhancer/Promoter Region Upstream of the Human Rhodopsin Kinase Gene.
J. E. Young, T. Vogt, K. W. Gross, and S. C. Khani (2003)
Invest. Ophthalmol. Vis. Sci. 44, 4076-4085
   Abstract »    Full Text »    PDF »
Sea urchin goosecoid function links fate specification along the animal-vegetal and oral-aboral embryonic axes.
L. M. Angerer, D. W. Oleksyn, A. M. Levine, X. Li, W. H. Klein, and R. C. Angerer (2001)
Development 128, 4393-4404
   Abstract »    Full Text »    PDF »
Functional Interaction of Jun and Homeodomain Proteins.
L. K. Schaefer, S. Wang, and T. S. Schaefer (2001)
J. Biol. Chem. 276, 43074-43082
   Abstract »    Full Text »    PDF »
Target Selectivity of Bicoid Is Dependent on Nonconsensus Site Recognition and Protein-Protein Interaction.
C. Zhao, V. Dave, F. Yang, T. Scarborough, and J. Ma (2000)
Mol. Cell. Biol. 20, 8112-8123
   Abstract »    Full Text »
Reprogrammable Recognition Codes in Bicoid Homeodomain-DNA Interaction.
V. Dave, C. Zhao, F. Yang, C.-S. Tung, and J. Ma (2000)
Mol. Cell. Biol. 20, 7673-7684
   Abstract »    Full Text »
Neuron-Specific Expression of the Rat Gonadotropin-Releasing Hormone Gene Is Conferred by Interactions of a Defined Promoter Element with the Enhancer in GT1-7 Cells.
S. B. Nelson, M. A. Lawson, C. G. Kelley, and P. L. Mellon (2000)
Mol. Endocrinol. 14, 1509-1522
   Abstract »    Full Text »
Site-specific Heterodimerization by Paired Class Homeodomain Proteins Mediates Selective Transcriptional Responses.
S. C. Tucker and R. Wisdom (1999)
J. Biol. Chem. 274, 32325-32332
   Abstract »    Full Text »    PDF »
The Mating-Type Proteins of Fission Yeast Induce Meiosis by Directly Activating mei3 Transcription.
W. J. van Heeckeren, D. R. Dorris, and K. Struhl (1998)
Mol. Cell. Biol. 18, 7317-7326
   Abstract »    Full Text »
Targeting gene expression to the head: the Drosophila orthodenticle gene is a direct target of the Bicoid morphogen.
Q Gao and R Finkelstein (1998)
Development 125, 4185-4193
   Abstract »    PDF »
The Xenopus Brachyury promoter is activated by FGF and low concentrations ofactivinandsuppressed by high concentrationsof activin and by paired-type homeodomain proteins.
B. V. Latinkic, M. Umbhauer, K. A. Neal, W. Lerchner, J. C. Smith, and V. Cunliffe (1997)
Genes & Dev. 11, 3265-3276
   Abstract »    Full Text »    PDF »
Genetic characterization of the role of the two HOX proteins, Proboscipedia and Sex Combs Reduced, in determination of adult antennal, tarsal, maxillary palp and proboscis identities in Drosophila melanogaster.
A Percival-Smith, J Weber, E Gilfoyle, and P Wilson (1997)
Development 124, 5049-5062
   Abstract »    PDF »
Ptx1, a bicoid-related homeo box transcription factor involved in transcription of the pro-opiomelanocortin gene..
T Lamonerie, J J Tremblay, C Lanctot, M Therrien, Y Gauthier, and J Drouin (1996)
Genes & Dev. 10, 1284-1295
   Abstract »    PDF »
In vivo requirement for the paired domain and homeodomain of the paired segmentation gene product.
C Bertuccioli, L Fasano, S Jun, S Wang, G Sheng, and C Desplan (1996)
Development 122, 2673-2685
   Abstract »    PDF »
Both the paired domain and homeodomain are required for in vivo function of Drosophila Paired.
P Miskiewicz, D Morrissey, Y Lan, L Raj, S Kessler, M Fujioka, T Goto, and M Weir (1996)
Development 122, 2709-2718
   Abstract »    PDF »
Altering cell fates in sea urchin embryos by overexpressing SpOtx, an orthodenticle-related protein.
C. Mao, A. Wikramanayake, L Gan, C. Chuang, R. Summers, and W. Klein (1996)
Development 122, 1489-1498
   Abstract »    PDF »
Crystal Structure of the MATa1/MATalpha2 Homeodomain Heterodimer Bound to DNA.
T. Li, M. R. Stark, A. D. Johnson, and C. Wolberger (1995)
Science 270, 262-269
   Abstract »    PDF »
Transcription Factor GATA-1 Regulates Human HOXB2 Gene Expression in Erythroid Cells.
I. Vieille-Grosjean and P. Huber (1995)
J. Biol. Chem. 270, 4544-4550
   Abstract »    Full Text »    PDF »
Ingrowth by photoreceptor axons induces transcription of a retrotransposon in the developing Drosophila brain.
B. Mozer and S Benzer (1994)
Development 120, 1049-1058
   Abstract »    PDF »
Cooperative dimerization of paired class homeo domains on DNA..
D Wilson, G Sheng, T Lecuit, N Dostatni, and C Desplan (1993)
Genes & Dev. 7, 2120-2134
   Abstract »    PDF »
Structure and DNA Binding of the Yeast MAT{alpha}2 Homeodomain.
C. Wolberger (1993)
Cold Spring Harb Symp Quant Biol 58, 159-166
   Abstract »    PDF »
Molecular genetics of aristaless, a prd-type homeo box gene involved in the morphogenesis of proximal and distal pattern elements in a subset of appendages in Drosophila..
K Schneitz, P Spielmann, and M Noll (1993)
Genes & Dev. 7, 114-129
   Abstract »    PDF »
Human and Drosophila Homeodomain Proteins That Enhance the DNA-Binding Activity of Serum Response Factor.
D. A. Grueneberg, S. Natesan, C. Alexandre, and M. Z. Gilman (1992)
Science 257, 1089-1095
   Abstract »    PDF »
Mapping functional specificity in the Dfd and Ubx homeo domains..
L Lin and W McGinnis (1992)
Genes & Dev. 6, 1071-1081
   Abstract »    PDF »
Identification of a Dominant Negative Homeodomain Mutation in Rieger Syndrome.
I. Saadi, E. V. Semina, B. A. Amendt, D. J. Harris, K. P. Murphy, J. C. Murray, and A. F. Russo (2001)
J. Biol. Chem. 276, 23034-23041
   Abstract »    Full Text »    PDF »
Altering the DNA-binding Specificity of the Yeast Matalpha 2 Homeodomain Protein.
J. R. Mathias, H. Zhong, Y. Jin, and A. K. Vershon (2001)
J. Biol. Chem. 276, 32696-32703
   Abstract »    Full Text »    PDF »
Exploring the DNA-binding specificities of zinc fingers with DNA microarrays.
M. L. Bulyk, X. Huang, Y. Choo, and G. M. Church (2001)
PNAS 98, 7158-7163
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)