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Science 14 December 1990:
Vol. 250. no. 4987, pp. 1573 - 1576
DOI: 10.1126/science.1703321
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Articles

Science, Vol 250, Issue 4987, 1573-1576
Copyright © 1990 by American Association for the Advancement of Science

articles

Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase

KL Gould, S Moreno, NK Tonks, and P Nurse

Department of Biochemistry, University of Oxford, United Kingdom.

The onset of M phase requires the activation of the pp34 protein kinase in all eukaryotes thus far examined. In Schizosaccharomyces pombe, pp34 is phosphorylated on Tyr15, and dephosphorylation of this residue regulates the initiation of mitosis. In this study, it is shown that dephosphorylation of Tyr15 triggered activation of the pp34-cyclin complex from fission yeast, that a human protein-tyrosine phosphatase can catalyze this event both in vitro and in vivo, and that activation of fission yeast pp34 does not require threonine dephosphorylation. The complementary DNA that encoded the tyrosine phosphatase replaced the mitotic activator p80cdc25, closely associating the cdc25(+)-activating pathway with tyrosine dephosphorylation of pp34.


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Cloning and Characterization of a Saccharomyces cerevisiae Gene Encoding the Low Molecular Weight Protein-tyrosine Phosphatase.
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Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation.
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A mouse cdc25 homolog is differentially and developmentally expressed..
A Kakizuka, B Sebastian, U Borgmeyer, I Hermans-Borgmeyer, J Bolado, T Hunter, M F Hoekstra, and R M Evans (1992)
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Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes.
E. Fischer, H Charbonneau, and N. Tonks (1991)
Science 253, 401-406
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Science. ISSN 0036-8075 (print), 1095-9203 (online)