Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 December 1990:
Vol. 250. no. 4987, pp. 1563 - 1566
DOI: 10.1126/science.2274788
Prev | Table of Contents | Next

Articles

Science, Vol 250, Issue 4987, 1563-1566
Copyright © 1990 by American Association for the Advancement of Science

articles

Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue

DL Scott, Z Otwinowski, MH Gelb, and PB Sigler

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.

The 2.0 angstroms crystal structure of a complex containing bee-venom phospholipase A2 (PLA2) and a phosphonate transition-state analogue was solved by multiple isomorphous replacement. The electron-density map is sufficiently detailed to visualize the proximal sugars of the enzyme's N-linked carbohydrate and a single molecule of the transition-state analogue bound ot its active center. Although bee-venom PLA2 does not belong to the large homologous Class I/II family that encompasses most other well-studied PLA2s, there is segmental sequence similarity and conservation of many functional substructures. Comparison of the bee-venom enzyme with other phospholipase structures provides compelling evidence for a common catalytic mechanism.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Crystal Structure of a Class XIB Phospholipase A2 (PLA2): RICE (ORYZA SATIVA) ISOFORM-2 PLA2 AND AN OCTANOATE COMPLEX.
J. E. Guy, U. Stahl, and Y. Lindqvist (2009)
J. Biol. Chem. 284, 19371-19379
   Abstract »    Full Text »    PDF »
Molecular Determinants for Interfacial Binding and Conformational Change in a Soluble Diacylglycerol Kinase.
A. Jerga, D. J. Miller, S. W. White, and C. O. Rock (2009)
J. Biol. Chem. 284, 7246-7254
   Abstract »    Full Text »    PDF »
A Molecular Mechanism for Lys49-Phospholipase A2 Activity Based on Ligand-induced Conformational Change.
A. L. B. Ambrosio, M. C. Nonato, H. S. S. de Araujo, R. Arni, R. J. Ward, C. L. Ownby, D. H. F. de Souza, and R. C. Garratt (2005)
J. Biol. Chem. 280, 7326-7335
   Abstract »    Full Text »    PDF »
Cellular Arachidonate-releasing Function of Novel Classes of Secretory Phospholipase A2s (Groups III and XII).
M. Murakami, S. Masuda, S. Shimbara, S. Bezzine, M. Lazdunski, G. Lambeau, M. H. Gelb, S. Matsukura, F. Kokubu, M. Adachi, et al. (2003)
J. Biol. Chem. 278, 10657-10667
   Abstract »    Full Text »    PDF »
Modulation of the Activity of Secretory Phospholipase A2 by Antimicrobial Peptides.
H. Zhao and P. K. J. Kinnunen (2003)
Antimicrob. Agents Chemother. 47, 965-971
   Abstract »    Full Text »    PDF »
Crystal Structure of a Complex Formed between a Snake Venom Phospholipase A2 and a Potent Peptide Inhibitor Phe-Leu-Ser-Tyr-Lys at 1.8 A Resolution.
V. Chandra, J. Jasti, P. Kaur, S. Dey, M. Perbandt, A. Srinivasan, Ch. Betzel, and T. P. Singh (2002)
J. Biol. Chem. 277, 41079-41085
   Abstract »    Full Text »    PDF »
Crystal Structure of Human Group X Secreted Phospholipase A2. ELECTROSTATICALLY NEUTRAL INTERFACIAL BINDING SURFACE TARGETS ZWITTERIONIC MEMBRANES.
Y. H. Pan, B.-Z. Yu, A. G. Singer, F. Ghomashchi, G. Lambeau, M. H. Gelb, M. K. Jain, and B. J. Bahnson (2002)
J. Biol. Chem. 277, 29086-29093
   Abstract »    Full Text »    PDF »
A Novel Prokaryotic Phospholipase A2. CHARACTERIZATION, GENE CLONING, AND SOLUTION STRUCTURE.
M. Sugiyama, K. Ohtani, M. Izuhara, T. Koike, K. Suzuki, S. Imamura, and H. Misaki (2002)
J. Biol. Chem. 277, 20051-20058
   Abstract »    Full Text »    PDF »
The Crystal Structure of Prokaryotic Phospholipase A2.
Y. Matoba, Y. Katsube, and M. Sugiyama (2002)
J. Biol. Chem. 277, 20059-20069
   Abstract »    Full Text »    PDF »
A Peptide Derived from Bee Venom-Secreted Phospholipase A2 Inhibits Replication of T-Cell Tropic HIV-1 Strains via Interaction with the CXCR4 Chemokine Receptor.
D. Fenard, G. Lambeau, T. Maurin, J.-C. Lefebvre, and A. Doglio (2001)
Mol. Pharmacol. 60, 341-347
   Abstract »    Full Text »    PDF »
Novel Human Secreted Phospholipase A2 with Homology to the Group III Bee Venom Enzyme.
E. Valentin, F. Ghomashchi, M. H. Gelb, M. Lazdunski, and G. Lambeau (2000)
J. Biol. Chem. 275, 7492-7496
   Abstract »    Full Text »    PDF »
Functional Characteristics of a Phospholipase A2 Inhibitor from Notechis ater Serum.
P. G. Hains, K.-L. Sung, A. Tseng, and K. W. Broady (2000)
J. Biol. Chem. 275, 983-991
   Abstract »    Full Text »    PDF »
Action of Human Group IIa Secreted Phospholipase A2 on Cell Membranes. VESICLE BUT NOT HEPARINOID BINDING DETERMINES RATE OF FATTY ACID RELEASE BY EXOGENOUSLY ADDED ENZYME.
R. S. Koduri, S. F. Baker, Y. Snitko, S. K. Han, W. Cho, D. C. Wilton, and M. H. Gelb (1998)
J. Biol. Chem. 273, 32142-32153
   Abstract »    Full Text »    PDF »
Docking Phospholipase A2 on Membranes Using Electrostatic Potential-Modulated Spin Relaxation Magnetic Resonance.
Y. Lin, R. Nielsen, D. Murray, W. L. Hubbell, C. Mailer, B. H. Robinson, and M. H. Gelb (1998)
Science 279, 1925-1929
   Abstract »    Full Text »    PDF »
A Cysteine-rich Domain Defined by a Novel Exon in a Slo Variant in Rat Adrenal Chromaffin Cells and PC12 Cells.
M. Saito, C. Nelson, L. Salkoff, and C. J. Lingle (1997)
J. Biol. Chem. 272, 11710-11717
   Abstract »    Full Text »    PDF »
Localization of Structural Elements of Bee Venom Phospholipase A2 Involved in N-type Receptor Binding and Neurotoxicity.
J.-P. Nicolas, Y. Lin, G. Lambeau, F. Ghomashchi, M. Lazdunski, and M. H. Gelb (1997)
J. Biol. Chem. 272, 7173-7181
   Abstract »    Full Text »    PDF »
Structural Aspects of Interfacial Adsorption. A CRYSTALLOGRAPHIC AND SITE-DIRECTED MUTAGENESIS STUDY OF THE PHOSPHOLIPASE A2 FROM THE VENOM OF AGKISTRODON PISCIVORUS PISCIVORUS.
S. K. Han, E. T. Yoon, D. L. Scott, P. B. Sigler, and W. Cho (1997)
J. Biol. Chem. 272, 3573-3582
   Abstract »    Full Text »    PDF »
Structural Elements of Secretory Phospholipases A(2) Involved in the Binding to M-type Receptors.
Gér. Lambeau, P. Ancian, J.-P. Nicolas, S. H. W. Beiboer, D. Moinier, H. Verheij, and M. Lazdunski (1995)
J. Biol. Chem. 270, 5534-5540
   Abstract »    Full Text »    PDF »
A Structure-Function Study of Bovine Pancreatic Phospholipase A(2) Using Polymerized Mixed Liposomes.
R. Dua, S.-K. Wu, and W. Cho (1995)
J. Biol. Chem. 270, 263-268
   Abstract »    Full Text »    PDF »
Structures of free and inhibited human secretory phospholipase A2 from inflammatory exudate.
D. Scott, S. White, J. Browning, J. Rosa, M. Gelb, and P. Sigler (1991)
Science 254, 1007-1010
   Abstract »    PDF »
Interfacial catalysis: the mechanism of phospholipase A2.
D. Scott, S. White, Z Otwinowski, W Yuan, M. Gelb, and P. Sigler (1990)
Science 250, 1541-1546
   Abstract »    PDF »
Crystal structure of cobra-venom phospholipase A2 in a complex with a transition-state analogue.
S. White, D. Scott, Z Otwinowski, M. Gelb, and P. Sigler (1990)
Science 250, 1560-1563
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)