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Science 7 December 1990:
Vol. 250. no. 4986, pp. 1416 - 1418
DOI: 10.1126/science.2255913
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Articles

Science, Vol 250, Issue 4986, 1416-1418
Copyright © 1990 by American Association for the Advancement of Science

articles

Requirement of ets-2 expression for Xenopus oocyte maturation

ZQ Chen, LA Burdett, AK Seth, JA Lautenberger, and TS Papas

Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702.

A molecular clone of the Xenopus laevis ets-2 gene was isolated from an oocyte complementary DNA library. The amount of messenger RNA (mRNA) in each oocyte or embryo was almost constant during oogenesis and was maintained until the blastula stage of embryonic development, indicating that the observed 3.2-kilobase transcript is a maternal message. The only normal adult tissue in which ets-2 mRNA was detected was the ovary. Injection of antisense oligonucleotides homologous to the ets-2 sequence into oocytes led to degradation of the mRNA and blocked hormone-induced germinal vesicle breakdown. The ets-2 product is thus required for the meiotic maturation of Xenopus oocytes.


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