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Science 30 November 1990:
Vol. 250. no. 4985, pp. 1278 - 1281
DOI: 10.1126/science.2244211
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Articles

Science, Vol 250, Issue 4985, 1278-1281
Copyright © 1990 by American Association for the Advancement of Science

articles

A borna virus cDNA encoding a protein recognized by antibodies in humans with behavioral diseases

S VandeWoude, JA Richt, MC Zink, R Rott, O Narayan, and JE Clements

Colorado State University, Lab Animal Resources, Fort Collins 80532.

Borna disease virus (BDV) causes a rare neurological disease in horses and sheep. The virus has not been classified because neither an infectious particle nor a specific nucleic acid had been identified. To identify the genome of BDV, a subtractive complementary DNA expression library was constructed with polyadenylate-selected RNA from a BDV-infected MDCK cell line. A clone (B8) was isolated that specifically hybridized to RNA isolated from BDV-infected brain tissue and BDV-infected cell lines. This clone hybridized to four BDV-specific positive strand RNAs (10.5, 3.6, 2.1, and 0.85 kilobases) and one negative strand RNA (10.5 kilobases) in BDV-infected rat brain. Nucleotide sequence analysis of the clone suggested that it represented a full-length messenger RNA which contained several open reading frames. In vitro transcription and translation of the clone resulted in the synthesis of the 14- and 24-kilodalton BDV-specific proteins. The 24-kilodalton protein, when translated in vitro from the clone, was recognized by antibodies in the sera of patients (three of seven) with behavioral disorders. This BDV-specific clone will provide the means to isolate the other BDV-specific nucleic acids and to identify the virus responsible for Borna disease. In addition, the significance of BDV or a BDV-related virus as a human pathogen can now be more directly examined.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Evidence of Borna disease virus genome detection in French domestic animals and in foxes (Vulpes vulpes).
G. Dauphin, V. Legay, C. Sailleau, S. Smondack, S. Hammoumi, and S. Zientara (2001)
J. Gen. Virol. 82, 2199-2204
   Abstract »    Full Text »    PDF »
Borna Disease Virus and Human Disease.
K. M. Carbone (2001)
Clin. Microbiol. Rev. 14, 513-527
   Abstract »    Full Text »    PDF »
Immunological and PCR Analyses for Borna Disease Virus in Psychiatric Patients and Blood Donors in Japan.
K. Fukuda, K. Takahashi, Y. Iwata, N. Mori, K. Gonda, T. Ogawa, K. Osonoe, M. Sato, S.-i. Ogata, T. Horimoto, et al. (2001)
J. Clin. Microbiol. 39, 419-429
   Abstract »    Full Text »
Isolation and Characterization of a New Subtype of Borna Disease Virus.
N. Nowotny, J. Kolodziejek, C. O. Jehle, A. Suchy, P. Staeheli, and M. Schwemmle (2000)
J. Virol. 74, 5655-5658
   Abstract »    Full Text »
Detection and Sequence Analysis of Borna Disease Virus p24 RNA from Peripheral Blood Mononuclear Cells of Patients with Mood Disorders or Schizophrenia and of Blood Donors.
Y. Iwata, K. Takahashi, X. Peng, K. Fukuda, K. Ohno, T. Ogawa, K. Gonda, N. Mori, S.-i. Niwa, and S. Shigeta (1998)
J. Virol. 72, 10044-10049
   Abstract »    Full Text »    PDF »
Borna Disease Virus-Induced Neurological Disorder in Mice: Infection of Neonates Results in Immunopathology.
W. Hallensleben, M. Schwemmle, J. Hausmann, L. Stitz, B. Volk, A. Pagenstecher, and P. Staeheli (1998)
J. Virol. 72, 4379-4386
   Abstract »    Full Text »    PDF »


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