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Science 23 November 1990:
Vol. 250. no. 4984, pp. 1132 - 1135
DOI: 10.1126/science.1701275

Articles

Science, Vol 250, Issue 4984, 1132-1135
Copyright © 1990 by American Association for the Advancement of Science


articles

Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells

G Walz, A Aruffo, W Kolanus, M Bevilacqua, and B Seed

Department of Genetics, Harvard Medical School, Boston, MA 02114.

Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-Lex) determinant, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-Lex or by proteins bearing sialyl-Lex, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-Lex.


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Synthesis of GDP-L-fucose by the Human FX Protein.
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alpha1,3-L-Fucosyltransferase Expression in Developing Human Myeloid Cells.
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Structure-Function Analysis of P-selectin-Sialyl Lewis^X Binding Interactions.
B. M. Revelle, D. Scott, T. P. Kogan, J. Zheng, and P. J. Beck (1996)
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Science 258, 964-969
   Abstract »    PDF »
Identification and Characterization of Three Novel beta 1,3-N-Acetylglucosaminyltransferases Structurally Related to the beta 1,3-Galactosyltransferase Family.
N. Shiraishi, A. Natsume, A. Togayachi, T. Endo, T. Akashima, Y. Yamada, N. Imai, S. Nakagawa, S. Koizumi, S. Sekine, et al. (2001)
J. Biol. Chem. 276, 3498-3507
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Science. ISSN 0036-8075 (print), 1095-9203 (online)