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Science 23 November 1990: Vol. 250. no. 4984, pp. 1132 - 1135 DOI: 10.1126/science.1701275
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Articles
Science, Vol 250, Issue 4984, 1132-1135
Copyright © 1990 by American Association for the Advancement of Science
Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells
G Walz,
A Aruffo,
W Kolanus,
M Bevilacqua,
and
B Seed
Department of Genetics, Harvard Medical School, Boston, MA 02114.
Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-Lex) determinant, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-Lex or by proteins bearing sialyl-Lex, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-Lex.
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