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Science 16 November 1990:
Vol. 250. no. 4983, pp. 988 - 991
DOI: 10.1126/science.1978413
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Articles

Science, Vol 250, Issue 4983, 988-991
Copyright © 1990 by American Association for the Advancement of Science

articles

Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

R DeGasperi, LJ Thomas, E Sugiyama, HM Chang, PJ Beck, P Orlean, C Albright, G Waneck, JF Sambrook, CD Warren, and al. et

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.

Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vitro Phosphorylation by cAMP-dependent Protein Kinase Up-regulates Recombinant Saccharomyces cerevisiae Mannosylphosphodolichol Synthase.
D. K. Banerjee, E. A. Carrasquillo, P. Hughey, J. S. Schutzbach, J. A. Martinez, and K. Baksi (2005)
J. Biol. Chem. 280, 4174-4181
   Abstract »    Full Text »    PDF »
A Homologue of Saccharomyces cerevisiae Dpm1p Is Not Sufficient for Synthesis of Dolichol-Phosphate-Mannose in Mammalian Cells.
S. Tomita, N. Inoue, Y. Maeda, K. Ohishi, J. Takeda, and T. Kinoshita (1998)
J. Biol. Chem. 273, 9249-9254
   Abstract »    Full Text »    PDF »
Protein C-Mannosylation Is Enzyme-catalysed and Uses Dolichyl-Phosphate-Mannose as a Precursor.
M.-A. Doucey, D. Hess, R. Cacan, and J. Hofsteenge (1998)
Mol. Biol. Cell 9, 291-300
   Abstract »    Full Text »
Human and Saccharomyces cerevisiae dolichol phosphate mannose synthases represent two classes of the enzyme, but both function in Schizosaccharomyces pombe.
P. A. Colussi, C. H. Taron, J. C. Mack, and P. Orlean (1997)
PNAS 94, 7873-7878
   Abstract »    Full Text »    PDF »
Expression Cloning of PIG-L, a Candidate N-Acetylglucosaminyl-phosphatidylinositol Deacetylase.
N. Nakamura, N. Inoue, R. Watanabe, M. Takahashi, J. Takeda, V. L. Stevens, and T. Kinoshita (1997)
J. Biol. Chem. 272, 15834-15840
   Abstract »    Full Text »    PDF »


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