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Science 16 November 1990:
Vol. 250. no. 4983, pp. 982 - 985
DOI: 10.1126/science.2237442
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Articles

Science, Vol 250, Issue 4983, 982-985
Copyright © 1990 by American Association for the Advancement of Science

articles

A cytoplasmic protein inhibits the GTPase activity of H-Ras in a phospholipid-dependent manner

MH Tsai, CL Yu, and DW Stacey

Department of Molecular Biology, Cleveland Clinic Foundation 44195.

A cytoplasmic protein has been identified that inhibits the guanosine triphosphatase (GTPase) activity of bacterially synthesized, cellular H-Ras protein. This GTPase inhibiting protein is able to counteract the activity of GTPase activating protein (GAP), which has been postulated to function as a negative regulator of Ras activity. The potential biological importance of the GTPase inhibiting protein is further supported by its interaction with lipids. Phospholipids produced in cells as a consequence of mitogenic stimulation increase the activity of the GTPase inhibiting protein, as well as inhibit the activity of GAP. The interaction of such lipids with each of these two regulatory proteins would, therefore, tend to increase the biological activity of Ras and stimulate cell proliferation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nuclear Transportation of Diacylglycerol Kinase {gamma} and Its Possible Function in the Nucleus.
T. Matsubara, Y. Shirai, K. Miyasaka, T. Murakami, Y. Yamaguchi, T. Ueyama, M. Kai, F. Sakane, H. Kanoh, T. Hashimoto, et al. (2006)
J. Biol. Chem. 281, 6152-6164
   Abstract »    Full Text »    PDF »
Dual Role of Reactive Oxygen Species in Vascular Growth.
K. K. Griendling and D. G. Harrison (1999)
Circ. Res. 85, 562-563
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Regulation of Phosphatidic Acid Phosphohydrolase by Epidermal Growth Factor. REDUCED ASSOCIATION WITH THE EGF RECEPTOR FOLLOWED BY INCREASED ASSOCIATION WITH PROTEIN KINASE Cepsilon.
Y. Jiang, Z. Lu, Q. Zang, and D. A. Foster (1996)
J. Biol. Chem. 271, 29529-29532
   Abstract »    Full Text »    PDF »
Molecular Cloning of a Novel Diacylglycerol Kinase Isozyme with a Pleckstrin Homology Domain and a C-terminal Tail Similar to Those of the EPH Family of Protein-tyrosine Kinases.
F. Sakane, S.-i. Imai, M. Kai, I. Wada, and H. Kanoh (1996)
J. Biol. Chem. 271, 8394-8401
   Abstract »    Full Text »    PDF »
Cytoskeleton--plasma membrane interactions.
E. Luna and A. Hitt (1992)
Science 258, 955-964
   Abstract »    PDF »
A GDP dissociation inhibitor that serves as a GTPase inhibitor for the Ras-like protein CDC42Hs.
M. Hart, Y Maru, D Leonard, O. Witte, T Evans, and R. Cerione (1992)
Science 258, 812-815
   Abstract »    PDF »
Regulation of Ras-GAP and the neurofibromatosis-1 gene product by eicosanoids.
J. Han, F McCormick, and I. Macara (1991)
Science 252, 576-579
   Abstract »    PDF »
Subtype-specific Translocation of Diacylglycerol Kinase alpha and gamma and Its Correlation with Protein Kinase C.
Y. Shirai, S. Segawa, M. Kuriyama, K. Goto, N. Sakai, and N. Saito (2000)
J. Biol. Chem. 275, 24760-24766
   Abstract »    Full Text »    PDF »


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