Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 16 November 1990:
Vol. 250. no. 4983, pp. 959 - 966
DOI: 10.1126/science.250.4983.959
Prev | Table of Contents | Next

Articles

The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants

Philip N. Benfey 1 and Nam-Hai Chua 2

1 Laboratory of Plant Molecular Biology, The Rockefeller University, New York, NY 10021
2 Laboratory of Plant Molecular Biology, The Rockefeller University, New York, NY 1002

Appropriate regulation of transcription in higher plants requires specific cis elements in the regulatory regions of genes and their corresponding trans-acting proteins. Analysis of the cauliflower mosaic virus (CaMV) 35S promoter has contributed to the understanding of transcriptional regulatory mechanisms. The intact 35S promoter confers constitutive expression upon heterologous genes in most plants. Dissection into subdomains that are able to confer tissue-specific gene expression has demonstrated that the promoter has a modular organization. When selected subdomains are combined, they confer expression not detected from the isolated subdomains, suggesting that synergistic interactions occur among cis elements. The expression patterns conferred by specific combinations of 35S subdomains differ in tobacco and petunia. This indicates that a combinatorial code of cisregulatory elements may be interpreted differently in different species.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Highly Boron Deficiency-Tolerant Plants Generated by Enhanced Expression of NIP5;1, a Boric Acid Channel.
Y. Kato, K. Miwa, J. Takano, M. Wada, and T. Fujiwara (2009)
Plant Cell Physiol. 50, 58-66
   Abstract »    Full Text »    PDF »
Effects of Transgenic Hybrid Aspen Overexpressing Polyphenol Oxidase on Rhizosphere Diversity.
K. L. Oliver, R. C. Hamelin, and W. E. Hintz (2008)
Appl. Envir. Microbiol. 74, 5340-5348
   Abstract »    Full Text »    PDF »
Transfer of Plastid DNA to the Nucleus Is Elevated during Male Gametogenesis in Tobacco.
A. E. Sheppard, M. A. Ayliffe, L. Blatch, A. Day, S. K. Delaney, N. Khairul-Fahmy, Y. Li, P. Madesis, A. J. Pryor, and J. N. Timmis (2008)
Plant Physiology 148, 328-336
   Abstract »    Full Text »    PDF »
Building Blocks for Plant Gene Assembly.
M. Karimi, A. Bleys, R. Vanderhaeghen, and P. Hilson (2007)
Plant Physiology 145, 1183-1191
   Abstract »    Full Text »    PDF »
MYB98 Positively Regulates a Battery of Synergid-Expressed Genes Encoding Filiform Apparatus Localized Proteins.
J. A. Punwani, D. S. Rabiger, and G. N. Drews (2007)
PLANT CELL 19, 2557-2568
   Abstract »    Full Text »    PDF »
Epigenetic Inactivation of Chalcone Synthase-A Transgene Transcription in Petunia Leads to a Reversion of the Post-Transcriptional Gene Silencing Phenotype.
A. Kanazawa, M. O'Dell, and R. P. Hellens (2007)
Plant Cell Physiol. 48, 638-647
   Abstract »    Full Text »    PDF »
Functional Analyses of Two Tomato APETALA3 Genes Demonstrate Diversification in Their Roles in Regulating Floral Development.
G. de Martino, I. Pan, E. Emmanuel, A. Levy, and V. F. Irish (2006)
PLANT CELL 18, 1833-1845
   Abstract »    Full Text »    PDF »
A variety of synergistic and antagonistic interactions mediated by cis-acting DNA motifs regulate gene expression in plant cells and modulate stability of the transcription complex formed on a basal promoter.
S. V. Sawant, K. Kiran, R. Mehrotra, C. P. Chaturvedi, S. A. Ansari, P. Singh, N. Lodhi, and R. Tuli (2005)
J. Exp. Bot. 56, 2345-2353
   Abstract »    Full Text »    PDF »
Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco.
N. Shirasawa-Seo, Y. Sano, S. Nakamura, T. Murakami, S. Seo, Y. Ohashi, Y. Hashimoto, and T. Matsumoto (2005)
J. Gen. Virol. 86, 1851-1860
   Abstract »    Full Text »    PDF »
Cell-to-Cell, but Not Long-Distance, Spread of RNA Silencing That Is Induced in Individual Epidermal Cells.
E. V. Ryabov, R. van Wezel, J. Walsh, and Y. Hong (2004)
J. Virol. 78, 3149-3154
   Abstract »    Full Text »    PDF »
Activation of Anthocyanin Biosynthesis in Gerbera hybrida (Asteraceae) Suggests Conserved Protein-Protein and Protein-Promoter Interactions between the Anciently Diverged Monocots and Eudicots.
P. Elomaa, A. Uimari, M. Mehto, V. A. Albert, R. A.E. Laitinen, and T. H. Teeri (2003)
Plant Physiology 133, 1831-1842
   Abstract »    Full Text »
Two Discrete cis Elements Control the Abaxial Side-Specific Expression of the FILAMENTOUS FLOWER Gene in Arabidopsis.
K. Watanabe and K. Okada (2003)
PLANT CELL 15, 2592-2602
   Abstract »    Full Text »    PDF »
Strategies for Development of Functionally Equivalent Promoters with Minimum Sequence Homology for Transgene Expression in Plants: cis-Elements in a Novel DNA Context versus Domain Swapping.
S. Bhullar, S. Chakravarthy, S. Advani, S. Datta, D. Pental, and P. K. Burma (2003)
Plant Physiology 132, 988-998
   Abstract »    Full Text »    PDF »
Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages.
R. S. Lamb and V. F. Irish (2003)
PNAS 100, 6558-6563
   Abstract »    Full Text »    PDF »
Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter.
S. H. Yang, H. Yu, and C. J. Goh (2002)
J. Exp. Bot. 53, 1899-1907
   Abstract »    Full Text »    PDF »
Overexpression of C4-cycle enzymes in transgenic C3 plants: a biotechnological approach to improve C3-photosynthesis.
R. E. Hausler, H.-J. Hirsch, F. Kreuzaler, and C. Peterhansel (2002)
J. Exp. Bot. 53, 591-607
   Abstract »    Full Text »    PDF »
Lipoxygenase H1 Gene Silencing Reveals a Specific Role in Supplying Fatty Acid Hydroperoxides for Aliphatic Aldehyde Production.
J. Leon, J. Royo, G. Vancanneyt, C. Sanz, H. Silkowski, G. Griffiths, and J. J. Sanchez-Serrano (2002)
J. Biol. Chem. 277, 416-423
   Abstract »    Full Text »
Tomato SP-Interacting Proteins Define a Conserved Signaling System That Regulates Shoot Architecture and Flowering.
L. Pnueli, T. Gutfinger, D. Hareven, O. Ben-Naim, N. Ron, N. Adir, and E. Lifschitz (2001)
PLANT CELL 13, 2687-2702
   Abstract »    Full Text »    PDF »
Networking Senescence-Regulating Pathways by Using Arabidopsis Enhancer Trap Lines.
Y. He, W. Tang, J. D. Swain, A. L. Green, T. P. Jack, and S. Gan (2001)
Plant Physiology 126, 707-716
   Abstract »    Full Text »    PDF »
Separable Whorl-Specific Expression and Negative Regulation by Enhancer Elements within the AGAMOUS Second Intron.
M. K. Deyholos and L. E. Sieburth (2000)
PLANT CELL 12, 1799-1810
   Abstract »    Full Text »
Negative Regulation of the SHATTERPROOF Genes by FRUITFULL During Arabidopsis Fruit Development.
C. Ferrándiz, S. J. Liljegren, and M. F. Yanofsky (2000)
Science 289, 436-438
   Abstract »    Full Text »
Transcriptional Activation of the Rice Tungro Bacilliform Virus Gene Is Critically Dependent on an Activator Element Located Immediately Upstream of the TATA Box.
X. He, T. Hohn, and J. Futterer (2000)
J. Biol. Chem. 275, 11799-11808
   Abstract »    Full Text »    PDF »
Activation of a Floral Homeotic Gene in Arabidopsis.
M. A. Busch, K. Bomblies, and D. Weigel (1999)
Science 285, 585-587
   Abstract »    Full Text »
Members of the YABBY gene family specify abaxial cell fate in Arabidopsis.
K. Siegfried, Y Eshed, S. Baum, D Otsuga, G. Drews, and J. Bowman (1999)
Development 126, 4117-4128
   Abstract »    PDF »
The Heat-Shock Element Is a Functional Component of the Arabidopsis APX1 Gene Promoter.
S. Storozhenko, P. De Pauw, M. Van Montagu, D. Inzé, and S. Kushnir (1998)
Plant Physiology 118, 1005-1014
   Abstract »    Full Text »
The SELF-PRUNING gene of tomato regulates vegetative to reproductive switching of sympodial meristems and is the ortholog of CEN and TFL1.
L Pnueli, L Carmel-Goren, D Hareven, T Gutfinger, J Alvarez, M Ganal, D Zamir, and E Lifschitz (1998)
Development 125, 1979-1989
   Abstract »    PDF »
The CArG boxes in the promoter of the Arabidopsis floral organ identity gene APETALA3 mediate diverse regulatory effects.
J. Tilly, D. Allen, and T Jack (1998)
Development 125, 1647-1657
   Abstract »    PDF »
Involvement of Maize Dof Zinc Finger Proteins in Tissue-Specific and Light-Regulated Gene Expression.
S. Yanagisawa and J. Sheen (1998)
PLANT CELL 10, 75-90
   Abstract »    Full Text »
Generation and assembly of secretory antibodies in plants.
J. Ma, A Hiatt, M Hein, N. Vine, F Wang, P Stabila, C van Dolleweerd, K Mostov, and T Lehner (1995)
Science 268, 716-719
   Abstract »    PDF »
Functional analysis of petunia floral homeotic MADS box gene pMADS1..
A R van der Krol, A Brunelle, S Tsuchimoto, and N H Chua (1993)
Genes & Dev. 7, 1214-1228
   Abstract »    PDF »
Root development in Arabidopsis: four mutants with dramatically altered root morphogenesis.
P. Benfey, P. Linstead, K Roberts, J. Schiefelbein, M. Hauser, and R. Aeschbacher (1993)
Development 119, 57-70
   Abstract »    PDF »
Polyhydroxybutyrate, a Biodegradable Thermoplastic, Produced in Transgenic Plants.
Y. Poirier, D. E. Dennis, K. Klomparens, and C. Somerville (1992)
Science 256, 520-523
   Abstract »    PDF »
Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements..
D Twell, J Yamaguchi, R A Wing, J Ushiba, and S McCormick (1991)
Genes & Dev. 5, 496-507
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)