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Science 9 November 1990:
Vol. 250. no. 4982, pp. 805 - 808
DOI: 10.1126/science.2237430
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Articles

Science, Vol 250, Issue 4982, 805-808
Copyright © 1990 by American Association for the Advancement of Science

articles

cdc2 gene expression at the G1 to S transition in human T lymphocytes

Y Furukawa, H Piwnica-Worms, TJ Ernst, Y Kanakura, and JD Griffin

Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.

The product of the cdc2 gene, designated p34cdc2, is a serine-threonine protein kinase that controls entry of eukaryotic cells into mitosis. Freshly isolated human T lymphocytes (G0 phase) were found to have very low amounts of p34cdc2 and cdc2 messenger RNA. Expression of cdc2 increased 18 to 24 hours after exposure of T cells to phytohemagglutinin, coincident with the G1 to S transition. Antisense oligodeoxynucleotides could reduce the increase in cdc2 expression and inhibited DNA synthesis, but had no effect on several early and mid-G1 events, including blastogenesis and expression of interleukin-2 receptors, transferrin receptors, c-myb, and c-myc. Induction of cdc2 required prior induction of c-myb and c-myc. These results suggest that cdc2 induction is part of an orderly sequence of events that occurs at the G1 to S transition in T cells.


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