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Science 9 November 1990:
Vol. 250. no. 4982, pp. 797 - 800
DOI: 10.1126/science.2237429
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Articles

Science, Vol 250, Issue 4982, 797-800
Copyright © 1990 by American Association for the Advancement of Science

articles

Methylation of an immediate-early inducible gene as a mechanism for B cell tolerance induction

VL Seyfert, SB McMahon, WD Glenn, AJ Yellen, VP Sukhatme, XM Cao, and JG Monroe

Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.

Stage-specific gene regulation is important in determining cell function during development. Immature B cells expressing membrane-bound immunoglobulin M (mIgM) are sensitive to antigen-induced tolerance, whereas mature B cells are activated by antigen. Previous studies have established an association between Egr-1 gene induction and antigen receptor (mIgM)-mediated activation of mature B cells. Here it is shown that the immature B cell line WEHI-231 and tolerance-sensitive bone marrow-derived B cells do not express Egr-1. It is further shown that lack of inducible expression in these cells is due to specific methylation of the Egr-1 gene. Thus, covalent inactivation of an activation-associated gene may explain tolerance sensitivity at specific stages of B cell development.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distinct Signal Thresholds for the Unique Antigen Receptor–linked Gene Expression Programs in Mature and Immature B Cells.
R. J. Benschop, D. Melamed, D. Nemazee, and J. C. Cambier (1999)
J. Exp. Med. 190, 749-756
   Abstract »    Full Text »    PDF »
The Transcription Factor Early Growth Response 1 (Egr-1) Advances Differentiation of Pre-B and Immature B Cells.
A. Dinkel, K. Warnatz, B. Ledermann, A. Rolink, P. F. Zipfel, K. Burki, and H. Eibel (1998)
J. Exp. Med. 188, 2215-2224
   Abstract »    Full Text »    PDF »
Neonatal Murine B Lymphocytes Respond to Polysaccharide Antigens in the Presence of IL-1 and IL-6.
R. L. Chelvarajan, N. L. Gilbert, and S. Bondada (1998)
J. Immunol. 161, 3315-3324
   Abstract »    Full Text »    PDF »
Phospholipase C beta  and Membrane Action of Calcitriol and Estradiol.
V. Le Mellay, B. Grosse, and M. Lieberherr (1997)
J. Biol. Chem. 272, 11902-11907
   Abstract »    Full Text »    PDF »
Induction of the Early Growth Response (Egr) Family of Transcription Factors during Thymic Selection.
H. Shao, D. H. Kono, L.-Y. Chen, E. M. Rubin, and J. Kaye (1997)
J. Exp. Med. 185, 731-744
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)