Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 2 November 1990:
Vol. 250. no. 4981, pp. 651 - 657
DOI: 10.1126/science.2146742
Prev | Table of Contents | Next

Articles

Science, Vol 250, Issue 4981, 651-657
Copyright © 1990 by American Association for the Advancement of Science

articles

Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

PM Kane, CT Yamashiro, DF Wolczyk, N Neff, M Goebl, and TH Stevens

Institute of Molecular Biology, University of Oregon, Eugene 97403.

The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H(+)-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5' and 3' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Posttranslational Protein Modification in Archaea.
J. Eichler and M. W. W. Adams (2005)
Microbiol. Mol. Biol. Rev. 69, 393-425
   Abstract »    Full Text »    PDF »
Involvement of the Nonhomologous Region of Subunit A of the Yeast V-ATPase in Coupling and in Vivo Dissociation.
E. Shao and M. Forgac (2004)
J. Biol. Chem. 279, 48663-48670
   Abstract »    Full Text »    PDF »
Evolution of divergent DNA recognition specificities in VDE homing endonucleases from two yeast species.
K. L. Posey, V. Koufopanou, A. Burt, and F. S. Gimble (2004)
Nucleic Acids Res. 32, 3947-3956
   Abstract »    Full Text »    PDF »
Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction.
A. Romanelli, A. Shekhtman, D. Cowburn, and T. W. Muir (2004)
PNAS 101, 6397-6402
   Abstract »    Full Text »    PDF »
Crystal Structure of a Mini-intein Reveals a Conserved Catalytic Module Involved in Side Chain Cyclization of Asparagine during Protein Splicing.
Y. Ding, M.-Q. Xu, I. Ghosh, X. Chen, S. Ferrandon, G. Lesage, and Z. Rao (2003)
J. Biol. Chem. 278, 39133-39142
   Abstract »    Full Text »    PDF »
Mutational Analysis of the Non-homologous Region of Subunit A of the Yeast V-ATPase.
E. Shao, T. Nishi, S. Kawasaki-Nishi, and M. Forgac (2003)
J. Biol. Chem. 278, 12985-12991
   Abstract »    Full Text »    PDF »
Karyopherin-Mediated Nuclear Import of the Homing Endonuclease VMA1-Derived Endonuclease Is Required for Self-Propagation of the Coding Region.
Y. Nagai, S. Nogami, F. Kumagai-Sano, and Y. Ohya (2003)
Mol. Cell. Biol. 23, 1726-1736
   Abstract »    Full Text »    PDF »
The First Putative Transmembrane Segment of Subunit c" (Vma16p) of the Yeast V-ATPase Is Not Necessary for Function.
T. Nishi, S. Kawasaki-Nishi, and M. Forgac (2003)
J. Biol. Chem. 278, 5821-5827
   Abstract »    Full Text »    PDF »
Investigating the endonuclease activity of four Pyrococcus abyssi inteins.
I. Saves, C. Morlot, L. Thion, J.-L. Rolland, J. Dietrich, and J.-M. Masson (2002)
Nucleic Acids Res. 30, 4158-4165
   Abstract »    Full Text »    PDF »
The Thy Pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases.
I. Saves, H. Eleaume, J. Dietrich, and J.-M. Masson (2000)
Nucleic Acids Res. 28, 4391-4396
   Abstract »    Full Text »    PDF »
In Vivo Splicing and Functional Characterization of Mycobacterium leprae RecA.
K. Frischkorn, B. Springer, E. C. Böttger, E. O. Davis, M. J. Colston, and P. Sander (2000)
J. Bacteriol. 182, 3590-3592
   Abstract »    Full Text »
Structural Insights into the Protein Splicing Mechanism of PI-SceI.
B. W. Poland, M.-Q. Xu, and F. A. Quiocho (2000)
J. Biol. Chem. 275, 16408-16413
   Abstract »    Full Text »    PDF »
Intronic GIY-YIG endonuclease gene in the mitochondrial genome of Podospora curvicolla: evidence for mobility.
C. Saguez, G. Lecellier, and F. Koll (2000)
Nucleic Acids Res. 28, 1299-1306
   Abstract »    Full Text »    PDF »
Inteins of Thermococcus fumicolans DNA Polymerase Are Endonucleases with Distinct Enzymatic Behaviors.
I. Saves, V. Ozanne, J. Dietrich, and J.-M. Masson (2000)
J. Biol. Chem. 275, 2335-2341
   Abstract »    Full Text »    PDF »
Probing the Structure of the PI-SceI-DNA Complex by Affinity Cleavage and Affinity Photocross-linking.
D. Hu, M. Crist, X. Duan, F. A. Quiocho, and F. S. Gimble (2000)
J. Biol. Chem. 275, 2705-2712
   Abstract »    Full Text »    PDF »
Cysteine Scanning Mutagenesis of the Noncatalytic Nucleotide Binding Site of the Yeast V-ATPase.
E. Vasilyeva, Q. Liu, K. J. MacLeod, J. D. Baleja, and M. Forgac (2000)
J. Biol. Chem. 275, 255-260
   Abstract »    Full Text »    PDF »
Composition and assembly of the yeast vacuolar H(+)-ATPase complex.
L. Graham, B Powell, and T. Stevens (2000)
J. Exp. Biol. 203, 61-70
   Abstract »
Photoaffinity Labeling of Wild-type and Mutant Forms of the Yeast V-ATPase A Subunit by 2-Azido-[32P]ADP.
K. J. MacLeod, E. Vasilyeva, K. Merdek, P. D. Vogel, and M. Forgac (1999)
J. Biol. Chem. 274, 32869-32874
   Abstract »    Full Text »    PDF »
Early Steps in Assembly of the Yeast Vacuolar H+-ATPase.
P. M. Kane, M. Tarsio, and J. Liu (1999)
J. Biol. Chem. 274, 17275-17283
   Abstract »    Full Text »    PDF »
Vacuolar and Plasma Membrane Proton-Adenosinetriphosphatases.
N. Nelson and W. R. Harvey (1999)
Physiol Rev 79, 361-385
   Abstract »    Full Text »    PDF »
Posttranscriptional Control of Gene Expression in Yeast.
J. E. G. McCarthy (1998)
Microbiol. Mol. Biol. Rev. 62, 1492-1553
   Abstract »    Full Text »    PDF »
Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.
H. Wu, Z. Hu, and X.-Q. Liu (1998)
PNAS 95, 9226-9231
   Abstract »    Full Text »    PDF »
Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein.
S. Chong, K. S. Williams, C. Wotkowicz, and M.-Q. Xu (1998)
J. Biol. Chem. 273, 10567-10577
   Abstract »    Full Text »    PDF »
Lightning strikes twice: Intron-intein coincidence.
V. Derbyshire and M. Belfort (1998)
PNAS 95, 1356-1357
   Full Text »    PDF »
Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta.
V. Lazarevic, B. Soldo, A. Dusterhoft, H. Hilbert, C. Mauel, and D. Karamata (1998)
PNAS 95, 1692-1697
   Abstract »    Full Text »    PDF »
Mutational Analysis of the Nucleotide Binding Sites of the Yeast Vacuolar Proton-translocating ATPase.
K. J. MacLeod, E. Vasilyeva, J. D. Baleja, and M. Forgac (1998)
J. Biol. Chem. 273, 150-156
   Abstract »    Full Text »    PDF »
A DnaB intein in Rhodothermus marinus: Indication of recent intein homing across remotely related organisms.
X.-Q. Liu and Z. Hu (1997)
PNAS 94, 7851-7856
   Abstract »    Full Text »    PDF »
Identification of Three Core Regions Essential for Protein Splicing of the Yeast Vma1 Protozyme. A RANDOM MUTAGENESIS STUDY OF THE ENTIRE VMA1-DERIVED ENDONUCLEASE SEQUENCE.
M. Kawasaki, S. Nogami, Y. Satow, Y. Ohya, and Y. Anraku (1997)
J. Biol. Chem. 272, 15668-15674
   Abstract »    Full Text »    PDF »
A Yeast ATP-binding Cassette-type Protein Mediating ATP-dependent Bile Acid Transport.
D. F. Ortiz, M. V. St. Pierre, A. Abdulmessih, and I. M. Arias (1997)
J. Biol. Chem. 272, 15358-15365
   Abstract »    Full Text »    PDF »
Site-directed Mutagenesis of the Yeast V-ATPase A Subunit.
Q. Liu, X.-H. Leng, P. R. Newman, E. Vasilyeva, P. M. Kane, and M. Forgac (1997)
J. Biol. Chem. 272, 11750-11756
   Abstract »    Full Text »    PDF »
Identification of an Unusual Intein in Chloroplast ClpP Protease of Chlamydomonas eugametos.
S. Wang and X.-Q. Liu (1997)
J. Biol. Chem. 272, 11869-11873
   Abstract »    Full Text »    PDF »
Subunit G of the Vacuolar Proton Pump. MOLECULAR CHARACTERIZATION AND FUNCTIONAL EXPRESSION.
B. P. Crider, P. Andersen, A. E. White, Z. Zhou, X. Li, J. P. Mattsson, L. Lundberg, D. J. Keeling, X.-S. Xie, D. K. Stone, et al. (1997)
J. Biol. Chem. 272, 10721-10728
   Abstract »    Full Text »    PDF »
Reconstitution of ATPase Activity from Individual Subunits of the Clathrin-coated Vesicle Proton Pump. THE REQUIREMENT AND EFFECT OF THREE SMALL SUBUNITS.
X.-S. Xie and X. S. Xie (1996)
J. Biol. Chem. 271, 30980-30985
   Abstract »    Full Text »    PDF »
Site-directed Mutagenesis of the 100-kDa Subunit (Vph1p) of the Yeast Vacuolar (H+)-ATPase.
X.-H. Leng, M. F. Manolson, Q. Liu, and M. Forgac (1996)
J. Biol. Chem. 271, 22487-22493
   Abstract »    Full Text »    PDF »
Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein. THE STEPS IN THE SPLICING PATHWAY, SIDE REACTIONS LEADING TO PROTEIN CLEAVAGE, AND ESTABLISHMENT OF AN IN VITRO SPLICING SYSTEM.
S. Chong, Y. Shao, H. Paulus, J. Benner, F. B. Perler, and M.-Q. Xu (1996)
J. Biol. Chem. 271, 22159-22168
   Abstract »    Full Text »    PDF »
Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase.
J. J. Tomashek, J. L. Sonnenburg, J. M. Artimovich, and D. J. Klionsky (1996)
J. Biol. Chem. 271, 10397-10404
   Abstract »    Full Text »    PDF »
Substitutions in Conserved Dodecapeptide Motifs That Uncouple the DNA Binding and DNA Cleavage Activities of PI-SceI Endonuclease.
F. S. Gimble and B. W. Stephens (1995)
J. Biol. Chem. 270, 5849-5856
   Abstract »    Full Text »    PDF »
Molecular biology of prion diseases.
S. Prusiner (1991)
Science 252, 1515-1522
   Abstract »    PDF »
Subunit D (Vma8p) of the Yeast Vacuolar H+-ATPase Plays a Role in Coupling of Proton Transport and ATP Hydrolysis.
T. Xu and M. Forgac (2000)
J. Biol. Chem. 275, 22075-22081
   Abstract »    Full Text »    PDF »
Regulation of Yeast Ectoapyrase Ynd1p Activity by Activator Subunit Vma13p of Vacuolar H+-ATPase.
X. Zhong, R. Malhotra, and G. Guidotti (2000)
J. Biol. Chem. 275, 35592-35599
   Abstract »    Full Text »    PDF »
Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein*.
I. Ghosh, L. Sun, and M.-Q. Xu (2001)
J. Biol. Chem. 276, 24051-24058
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)