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Science 2 November 1990:
Vol. 250. no. 4981, pp. 651 - 657
DOI: 10.1126/science.2146742
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Articles

Science, Vol 250, Issue 4981, 651-657
Copyright © 1990 by American Association for the Advancement of Science

articles

Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

PM Kane, CT Yamashiro, DF Wolczyk, N Neff, M Goebl, and TH Stevens

Institute of Molecular Biology, University of Oregon, Eugene 97403.

The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H(+)-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5' and 3' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)