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Science 26 October 1990:
Vol. 250. no. 4980, pp. 543 - 546
DOI: 10.1126/science.2237404
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Articles

Science, Vol 250, Issue 4980, 543-546
Copyright © 1990 by American Association for the Advancement of Science

articles

Tetraplex formation of a guanine-containing nonameric DNA fragment

RZ Jin, KJ Breslauer, RA Jones, and BL Gaffney

Department of Chemistry, Rutgers, State University of New Jersey, Piscataway 08855.

A combination of spectroscopic and calorimetric techniques has been used to characterize the structures formed by a family of short, guanine-containing DNA single strands of the form d[GGTTXTTGG], X = A, C, G, T. In 1 molar NaCl at low temperatures, these molecules do not behave like single strands, but rather exhibit properties consistent with tetraplex formation. The standard state enthalpies, entropies, and free energies for formation of each tetraplex have been measured, as have preliminary nuclear magnetic resonance (NMR) spectra. In 1 molar KCl, the melting behavior of the structure or structures is more complex than in 1 molar NaCl. This observation may be related to the recently proposed "sodium-potassium switch."


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Nucleic acid specificity of a vertebrate telomere-binding protein: evidence for G-G base pair recognition at the core-binding site..
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