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Science 12 October 1990:
Vol. 250. no. 4978, pp. 271 - 273
DOI: 10.1126/science.2218529
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Articles

Science, Vol 250, Issue 4978, 271-273
Copyright © 1990 by American Association for the Advancement of Science

articles

Cleaving yeast and Escherichia coli genomes at a single site

M Koob and W Szybalski

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.

The 15-megabase pair Saccharomyces cerevisiae and the 4.7-megabase pair Escherichia coli genomes were completely cleaved at a single predetermined site by means of the Achilles' heel cleavage (AC) procedure. The symmetric lac operator (lacOs) was introduced into the circular Escherichia coli genome and into one of the 16 yeast chromosomes. Intact chromosomes from the resulting strains were prepared in agarose microbeads and methylated with Hha I (5'-GCGC) methyltransferase (M.Hha I) in the presence of lac repressor (LacI). All Hae II sites (5'-[sequence: see text]) with the exception of the one in lacOs, which was protected by LacI, were modified and thus no longer recognized by Hae II. After inactivation of M.Hha I and LacI, Hae II was used to completely cleave the chromosomes specifically at the inserted lacOs. These experiments demonstrate the feasibility of using the AC approach to efficiently extend the specificity of naturally occurring restriction enzymes and create new tools for the mapping and precise molecular dissection of multimegabase genomes.


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