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Science 12 October 1990:
Vol. 250. no. 4978, pp. 245 - 250
DOI: 10.1126/science.2218528

Articles

Science, Vol 250, Issue 4978, 245-250
Copyright © 1990 by American Association for the Advancement of Science


articles

Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes

DR Cox, M Burmeister, ER Price, S Kim, and RM Myers

Department of Psychiatry, University of California, San Francisco 94143.

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.


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D. M. Church, J. Yang, M. Bocian, R. Shiang, and J. J. Wasmuth (1997)
Genome Res. 7, 787-801
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Molecular Cloning, Mapping to Human Chromosome 1q21-q23, and Cell Binding Characteristics of Spalpha , a New Member of the Scavenger Receptor Cysteine-rich (SRCR) Family of Proteins.
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G G Bouffard, L M Iyer, J R Idol, V V Braden, A F Cunningham, L A Weintraub, R M Mohr-Tidwell, D C Peluso, R S Fulton, M P Leckie, et al. (1997)
Genome Res. 7, 59-64
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Statistical methods for gene map construction by fluorescence in situ hybridization..
S W Guo and W L Flejter (1996)
Genome Res. 6, 1133-1148
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Hybrid selection as a method of increasing mapping power for radiation hybrids..
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An STS-Based Map of the Human Genome.
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Human repeat-mediated integration of selectable markers into somatic cell hybrids..
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Genome Res. 5, 444-452
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A radiation hybrid map of 40 loci for the distal long arm of human chromosome 8..
T B Lewis, L Nelson, K Ward, and R J Leach (1995)
Genome Res. 5, 334-341
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A Time to Sequence.
M. V. Olson (1995)
Science 270, 394
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The Genexpress Index: a resource for gene discovery and the genic map of the human genome..
R Houlgatte, R Mariage-Samson, S Duprat, A Tessier, S Bentolila, B Lamy, and C Auffray (1995)
Genome Res. 5, 272-304
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Statistical methods for polyploid radiation hybrid mapping..
K Lange, M Boehnke, D R Cox, and K L Lunetta (1995)
Genome Res. 5, 136-150
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Experimental design and error detection for polyploid radiation hybrid mapping..
K L Lunetta, M Boehnke, K Lange, and D R Cox (1995)
Genome Res. 5, 151-163
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Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation..
R G Del Mastro, L Wang, A D Simmons, T D Gallardo, G A Clines, J A Ashley, C J Hilliard, J J Wasmuth, J D McPherson, and M Lovett (1995)
Genome Res. 5, 185-194
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Regional assignment of 68 new human gene transcripts on chromosome 11..
M F Rosier, I Reguigne-Arnould, P Couillin, M D Devignes, and C Auffray (1995)
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Assessing mapping progress in the Human Genome Project.
D. Cox, E. Green, E. Lander, D Cohen, and R. Myers (1994)
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Criterion for the Completeness of Large-scale Physical Maps of DNA.
M.V. Olson and P. Green (1993)
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Genome analysis and the human X chromosome.
J. Mandel, A. Monaco, D. Nelson, D Schlessinger, and H Willard (1992)
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Sequence-tagged site (STS) content mapping of human chromosomes: theoretical considerations and early experiences..
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Molecular Cloning and Expression of Two Distinct Human Chondroitin 4-O-Sulfotransferases That Belong to the HNK-1 Sulfotransferase Gene Family.
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Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.
R.-M. Hu, Z.-G. Han, H.-D. Song, Y.-D. Peng, Q.-H. Huang, S.-X. Ren, Y.-J. Gu, C.-H. Huang, Y.-B. Li, C.-L. Jiang, et al. (2000)
PNAS 97, 9543-9548
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