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Science 12 October 1990: Vol. 250. no. 4978, pp. 245 - 250 DOI: 10.1126/science.2218528
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Articles
Science, Vol 250, Issue 4978, 245-250
Copyright © 1990 by American Association for the Advancement of Science
Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes
DR Cox,
M Burmeister,
ER Price,
S Kim,
and
RM Myers
Department of Psychiatry, University of California, San Francisco 94143.
Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.
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