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Science 28 September 1990:
Vol. 249. no. 4976, pp. 1561 - 1564
DOI: 10.1126/science.2218497
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Articles

Science, Vol 249, Issue 4976, 1561-1564
Copyright © 1990 by American Association for the Advancement of Science

articles

"Pure" human hematopoietic progenitors: permissive action of basic fibroblast growth factor

M Gabbianelli, M Sargiacomo, E Pelosi, U Testa, G Isacchi, and C Peschle

Department of Hematology and Oncology, Istituto Superiore di Sanita, Rome, Italy.

Methodology has been developed that enables virtually complete purification and abundant recovery of early hematopoietic progenitors from normal human adult peripheral blood. A fraction of the pure progenitors is multipotent (generates mixed colonies) and exhibits self-renewal capacity (gives rise to blast cell colonies). This methodology provides a fundamental tool for basic and clinical studies on hematopoiesis. Optimal in vitro cloning of virtually pure progenitors requires not only the stimulatory effect of interleukin-3, granulocyte-macrophage colony-stimulating factor, and erythropoietin, but also the permissive action of basic fibroblast growth factor. These findings suggest a regulatory role for this growth factor in early hematopoiesis.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)