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Science 17 August 1990:
Vol. 249. no. 4970, pp. 799 - 802
DOI: 10.1126/science.2389145
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Articles

Science, Vol 249, Issue 4970, 799-802
Copyright © 1990 by American Association for the Advancement of Science

articles

Target-dependent structural changes accompanying long-term synaptic facilitation in Aplysia neurons

DL Glanzman, ER Kandel, and S Schacher

Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, NY 10032.

The mechanisms underlying structural changes that accompany learning and memory have been difficult to investigate in the intact nervous system. In order to make these changes more accessible for experimental analysis, dissociated cell culture and low-light-level video microscopy were used to examine Aplysia sensory neurons in the presence or absence of their target cells. Repeated applications of serotonin, a facilitating transmitter important in behavioral dishabituation and sensitization, produced growth of the sensory neurons that paralleled the long-term enhancement of synaptic strength. This growth required the presence of the postsynaptic motor neuron. Thus, both the structural changes and the synaptic facilitation of Aplysia sensorimotor synapses accompanying long-term behavioral sensitization can be produced in vitro by applying a single facilitating transmitter repeatedly. These structural changes depend on an interaction of the presynaptic neuron with an appropriate postsynaptic target.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Long-term regulation of neuronal high-affinity glutamate and glutamine uptake in Aplysia.
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