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Science 17 August 1990:
Vol. 249. no. 4970, pp. 783 - 786
DOI: 10.1126/science.1697102
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Articles

Science, Vol 249, Issue 4970, 783-786
Copyright © 1990 by American Association for the Advancement of Science

articles

External guide sequences for an RNA enzyme

AC Forster and S Altman

Department of Biology, Yale University, New Haven, CT 06520.

Ribonuclease P (RNase P) from Escherichia coli or its catalytic RNA subunit can efficiently cleave small RNA substrates that lack the conserved features of natural substrates of RNase P if an additional small RNA is also present. This additional RNA must contain a sequence complementary to the substrate [external guide sequence (EGS)] and a 3'-proximal CCA sequence to ensure cleavage. The aminoacyl acceptor stem and some additional 5'- and 3'-terminal sequences of a precursor transfer RNA are sufficient to allow efficient cleavage by RNAase P, and the 2'-hydroxyl group at the cleavage site is not absolutely necessary for cleavage. In principle, any RNA could be targeted by a custom-designed EGS RNA for specific cleavage by RNase P in vitro or in vivo.


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