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Science 17 August 1990:
Vol. 249. no. 4970, pp. 781 - 783
DOI: 10.1126/science.2389144
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Articles

Science, Vol 249, Issue 4970, 781-783
Copyright © 1990 by American Association for the Advancement of Science

articles

Antibody-catalyzed porphyrin metallation

AG Cochran and PG Schultz

Department of Chemistry, University of California, Berkeley 94720.

An antibody elicited to a distorted N-methyl porphyrin catalyzed metal ion chelation by the planar porphyrin. At fixed Zn2+ and Cu2+ concentrations, the antibody-catalyzed reaction showed saturation kinetics with respect to the substrate mesoporphyrin IX (2) and was inhibited by the hapten, N-methylmesoporphyrin IX (1). The turnover number of 80 hour-1 for antibody-catalyzed metallation of 2 with Zn2+ compares with an estimated value of 800 hour-1 for ferrochelatase. The antibody also catalyzed the insertion of Co2+ and Mn2+ into 2, but it did not catalyze the metallation of protoporphyrin IX (3) or deuteroporphyrin IX (4). The antibody has high affinity for several metalloporphyrins, suggesting an approach to developing antibody-heme catalysts for redox or electron transfer reactions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Metal Ion Substrate Inhibition of Ferrochelatase.
G. A. Hunter, M. P. Sampson, and G. C. Ferreira (2008)
J. Biol. Chem. 283, 23685-23691
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A Metalloantibody That Irreversibly Binds a Protein Antigen.
K. Trisler, L. L. Looger, V. Sharma, M. Baker, D. E. Benson, S. Trauger, P. G. Schultz, and V. V. Smider (2007)
J. Biol. Chem. 282, 26344-26353
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Substrate interactions with human ferrochelatase.
A. Medlock, L. Swartz, T. A. Dailey, H. A. Dailey, and W. N. Lanzilotta (2007)
PNAS 104, 1789-1793
   Abstract »    Full Text »    PDF »
Structural evidence for substrate strain in antibody catalysis.
J. Yin, S. E. Andryski, A. E. Beuscher IV, R. C. Stevens, and P. G. Schultz (2003)
PNAS 100, 856-861
   Abstract »    Full Text »    PDF »
From molecular diversity to catalysis: lessons from the immune system.
P. Schultz and R. Lerner (1995)
Science 269, 1835-1842
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At the crossroads of chemistry and immunology: catalytic antibodies.
R. Lerner, S. Benkovic, and P. Schultz (1991)
Science 252, 659-667
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