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Science 3 August 1990:
Vol. 249. no. 4968, pp. 553 - 556
DOI: 10.1126/science.2166336
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Articles

Science, Vol 249, Issue 4968, 553-556
Copyright © 1990 by American Association for the Advancement of Science

articles

Protein tyrosine phosphatase activity of an essential virulence determinant in Yersinia

KL Guan and JE Dixon

Department of Biochemistry, Purdue University, West Lafayette, IN 47907.

Yersinia is the genus of bacteria that is the causative agent in plague or the black death, and on several occasions this organism has killed a significant portion of the world's population. An essential virulence determinant of Yersinia was shown to be a protein tyrosine phosphatase. The recombinant 50-kilodalton Yersinia phosphatase had a specificity for removal of phosphate from Tyr-containing as opposed to Ser/Thr-containing phosphopeptides and proteins. Site-directed mutagenesis was used to show that the Yersinia phosphatase possesses an essential Cys residue required for catalysis. Amino acids surrounding an essential Cys residue are highly conserved, as are other amino acids in the Yersinia and mammalian protein tyrosine phosphatases, suggesting that they use a common catalytic mechanism.


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P. Chiarugi, P. Cirri, F. Marra, G. Raugei, T. Fiaschi, G. Camici, G. Manao, R. G. Romanelli, and G. Ramponi (1998)
J. Biol. Chem. 273, 6776-6785
   Abstract »    Full Text »    PDF »
Altering the Nucleophile Specificity of a Protein-tyrosine Phosphatase-catalyzed Reaction. PROBING THE FUNCTION OF THE INVARIANT GLUTAMINE RESIDUES.
Y. Zhao, L. Wu, S. J. Noh, K.-L. Guan, and Z.-Y. Zhang (1998)
J. Biol. Chem. 273, 5484-5492
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Differential regulation of FUS3 MAP kinase by tyrosine-specific phosphatases PTP2/PTP3 and dual-specificity phosphatase MSG5 in Saccharomyces cerevisiae..
X L Zhan, R J Deschenes, and K L Guan (1997)
Genes & Dev. 11, 1690-1702
   Abstract »    PDF »
Yersinia enterocolitica Promotes Deactivation of Macrophage Mitogen-activated Protein Kinases Extracellular Signal-regulated Kinase-1/2, p38, and c-Jun NH2-terminal Kinase. CORRELATION WITH ITS INHIBITORY EFFECT ON TUMOR NECROSIS FACTOR-alpha PRODUCTION.
K. Ruckdeschel, J. Machold, A. Roggenkamp, S. Schubert, J. Pierre, R. Zumbihl, J.-P. Liautard, J. Heesemann, and B. Rouot (1997)
J. Biol. Chem. 272, 15920-15927
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Phosphorylation Events Modulate the Ability of Interferon Consensus Sequence Binding Protein to Interact with Interferon Regulatory Factors and to Bind DNA.
R. Sharf, D. Meraro, A. Azriel, A. M. Thornton, K. Ozato, E. F. Petricoin, A. C. Larner, F. Schaper, H. Hauser, and B.-Z. Levi (1997)
J. Biol. Chem. 272, 9785-9792
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Mitogen-activated Protein Kinase Phosphatases Inactivate Stress-activated Protein Kinase Pathways in Vivo.
D. D. Hirsch and P. J.S. Stork (1997)
J. Biol. Chem. 272, 4568-4575
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Protein-Tyrosine Phosphatase 1B Complexes with the Insulin Receptor in Vivo and Is Tyrosine-phosphorylated in the Presence of Insulin.
D. Bandyopadhyay, A. Kusari, K. A. Kenner, F. Liu, J. Chernoff, T. A. Gustafson, and J. Kusari (1997)
J. Biol. Chem. 272, 1639-1645
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Interferon gamma -dependent Induction of Human Intercellular Adhesion Molecule-1 Gene Expression Involves Activation of a Distinct STAT Protein Complex.
S. M. Naik, N. Shibagaki, L.-J. Li, K. L. Quinlan, L. L.L. Paxton, and S. W. Caughman (1997)
J. Biol. Chem. 272, 1283-1290
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p75, a Member of the Heat Shock Protein Family, Undergoes Tyrosine Phosphorylation in Response to Oxidative Stress.
Y. R. Hadari, H. U. Haring, and Y. Zick (1997)
J. Biol. Chem. 272, 657-662
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Cloning and Characterization of Islet Cell Antigen-related Protein-tyrosine Phosphatase (PTP), a Novel Receptor-like PTP and Autoantigen in Insulin-dependent Diabetes.
L. Cui, W.-P. Yu, H. J. DeAizpurua, R. S. Schmidli, and C. J. Pallen (1996)
J. Biol. Chem. 271, 24817-24823
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Identification of a Bacterial Inhibitor of Protein Kinases. MECHANISM AND ROLE IN HOST CELL INVASION.
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J. Biol. Chem. 271, 23431-23437
   Abstract »    Full Text »    PDF »
The X-ray Crystal Structures of Yersinia Tyrosine Phosphatase with Bound Tungstate and Nitrate. MECHANISTIC IMPLICATIONS.
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J. Biol. Chem. 271, 18780-18788
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The Active Site Specificity of the Yersinia Protein-tyrosine Phosphatase.
D. Dunn, L. Chen, D. S. Lawrence, and Z.-Y. Zhang (1996)
J. Biol. Chem. 271, 168-173
   Abstract »    Full Text »    PDF »
Are Protein-tyrosine Phosphatases Specific for Phosphotyrosine?.
Z.-Y. Zhang and Z. Y. Zhang (1995)
J. Biol. Chem. 270, 16052-16055
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Crystal structure of human protein tyrosine phosphatase 1B.
D Barford, A. Flint, and N. Tonks (1994)
Science 263, 1397-1404
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Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
G. Martin, S. Brommonschenkel, J Chunwongse, A Frary, M. Ganal, R Spivey, T Wu, E. Earle, and S. Tanksley (1993)
Science 262, 1432-1436
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Mechanism-based inactivation of prostatic acid phosphatase.
J. Myers and T. Widlanski (1993)
Science 262, 1451-1453
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Cyclin D1 is a nuclear protein required for cell cycle progression in G1..
V Baldin, J Lukas, M J Marcote, M Pagano, and G Draetta (1993)
Genes & Dev. 7, 812-821
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Science 253, 401-406
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Biologists turn on to "off-enzymes".
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Science 251, 744-746
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Identification of Residues in the N-terminal Domain of the Yersinia Tyrosine Phosphatase That Are Critical for Substrate Recognition.
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J. Biol. Chem. 276, 5005-5011
   Abstract »    Full Text »    PDF »
A distinctive role for the Yersinia protein kinase: Actin binding, kinase activation, and cytoskeleton disruption.
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PNAS 97, 9431-9436
   Abstract »    Full Text »    PDF »


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